基于在位光还原银胶法的表面增强拉曼散射技术检测核酸碱基
在环状有机物存在的条件下,Ag＋经激光诱导可发生光还原反应,形成Ag原子聚集的银胶体。本研究通过拉曼散射光谱、紫外-可见光谱和扫描电镜证明,核酸碱基和Ag＋混合溶液经过激光诱导可以生成银胶体颗粒。随着银胶体的生成,核酸碱基的拉曼光谱信号因为表面增强拉曼散射效应而得到明显增强。实验表明,本方法不但可以获得高信噪比的拉曼光谱谱图,而且获得的胞嘧啶、尿嘧啶、胸腺嘧啶、腺嘌呤和鸟嘌呤的增强拉曼光谱与Lee-Meisel银胶和银膜表面两种体系下获得的表面增强拉曼光谱具有相似性。同时,腺嘌呤和鸟嘌呤又出现了一些因Ag＋作用而产生的新位移谱带,但是仍然可以作为指征碱基类别的特征峰归属。利用Ag＋光还原的方法...
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| Published in | 分析化学 Vol. 39; no. 8; pp. 1159 - 1165 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
吉林大学分子酶学工程教育部重点实验室,长春,130012%吉林大学生命科学学院,长春,130012%吉林省长春市疾病预防控制中心,长春,130033%长春医学高等专科学校,长春,130013
2011
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2011.01159 |
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| Abstract | 在环状有机物存在的条件下,Ag＋经激光诱导可发生光还原反应,形成Ag原子聚集的银胶体。本研究通过拉曼散射光谱、紫外-可见光谱和扫描电镜证明,核酸碱基和Ag＋混合溶液经过激光诱导可以生成银胶体颗粒。随着银胶体的生成,核酸碱基的拉曼光谱信号因为表面增强拉曼散射效应而得到明显增强。实验表明,本方法不但可以获得高信噪比的拉曼光谱谱图,而且获得的胞嘧啶、尿嘧啶、胸腺嘧啶、腺嘌呤和鸟嘌呤的增强拉曼光谱与Lee-Meisel银胶和银膜表面两种体系下获得的表面增强拉曼光谱具有相似性。同时,腺嘌呤和鸟嘌呤又出现了一些因Ag＋作用而产生的新位移谱带,但是仍然可以作为指征碱基类别的特征峰归属。利用Ag＋光还原的方法在位获得银溶胶增强拉曼光谱信号,具有银胶制备操作简单、峰位特征等优点,在核酸碱基的低浓度在位检测方面具有应用前景。 |
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| AbstractList | O657.37; 在环状有机物存在的条件下,Ag+经激光诱导可发生光还原反应,形成Ag原子聚集的银胶体.本研究通过拉曼散射光谱、紫外-可见光谱和扫描电镜证明,核酸碱基和Ag+混合溶液经过激光诱导可以生成银胶体颗粒.随着银胶体的生成,核酸碱基的拉曼光谱信号因为表面增强拉曼散射效应而得到明显增强.实验表明,本方法不但可以获得高信噪比的拉曼光谱谱图,而且获得的胞嘧啶、尿嘧啶、胸腺嘧啶、腺嘌呤和鸟嘌呤的增强拉曼光谱与Lee-Meisel银胶和银膜表面两种体系下获得的表面增强拉曼光谱具有相似性.同时,腺嚓吟和鸟嘌呤又出现了一些因Ag+作用而产生的新位移谱带,但是仍然可以作为指征碱基类别的特征峰归属.利用Ag+光还原的方法在位获得银溶胶增强拉曼光谱信号,具有银胶制备操作简单、峰位特征等优点,在核酸碱基的低浓度在位检测方面具有应用前景. 在环状有机物存在的条件下,Ag＋经激光诱导可发生光还原反应,形成Ag原子聚集的银胶体。本研究通过拉曼散射光谱、紫外-可见光谱和扫描电镜证明,核酸碱基和Ag＋混合溶液经过激光诱导可以生成银胶体颗粒。随着银胶体的生成,核酸碱基的拉曼光谱信号因为表面增强拉曼散射效应而得到明显增强。实验表明,本方法不但可以获得高信噪比的拉曼光谱谱图,而且获得的胞嘧啶、尿嘧啶、胸腺嘧啶、腺嘌呤和鸟嘌呤的增强拉曼光谱与Lee-Meisel银胶和银膜表面两种体系下获得的表面增强拉曼光谱具有相似性。同时,腺嘌呤和鸟嘌呤又出现了一些因Ag＋作用而产生的新位移谱带,但是仍然可以作为指征碱基类别的特征峰归属。利用Ag＋光还原的方法在位获得银溶胶增强拉曼光谱信号,具有银胶制备操作简单、峰位特征等优点,在核酸碱基的低浓度在位检测方面具有应用前景。 |
| Author | 吴雷 李海超 赵海峰 孙宇 许浩然 吕明 杨春花 李文钊 李正强 |
| AuthorAffiliation | 吉林大学分子酶学工程教育部重点实验室,长春130012 吉林大学生命科学学院,长春130012 吉林省长春市疾病预防控制中心,长春130033 长春医学高等专科学校,长春130013 |
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| Author_FL | ZHAO Hai-Feng XU Hao-Ran YANG Chun-Hua LU Ming SUN Yu LI Wen-Zhao LI Zheng-Qiang WU Lei LI Hai-Chao |
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| DocumentTitleAlternate | Detection of Nucleic Acid Bases by Technique Based on in-situ Surface Enhanced Raman Scattering Photo-reduced Silver Colloids |
| DocumentTitle_FL | Detection of Nucleic Acid Bases by Surface Enhanced Raman Scattering Technique Based on in-situ Photo-reduced Silver Colloids |
| EndPage | 1165 |
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| Keywords | 碱基检测 光还原反应 银胶体 表面增强拉曼散射 |
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| Notes | WU Lei,LI Hai-Chao,ZHAO Hai-Feng,SUN Yu,XU Hao-Ran,LU Ming, YANG Chun-Hua,LI Wen-Zhao,LI Zheng-Qiang 1（Key Laboratory for Molecular Enzymology and Engineering,Ministry of Education, Jilin University,Changchun 130012） 2（College of life Science,Jilin Univisity,Changchun 130012） 3（Changchun City Illness Defend Control Center,Changchun 130033） 4（Changchun Medical College,Changchun 130013） Photo-reduction reaction;Silver colloidal;Surface enhanced Raman scattering;Detection of nucleic acids bases 22-1125/O6 The formation of colloids induced by laser irradiation was proved by the means of Raman scattering,UV-Vis spectrum and transmission electron microscopy（TEM）.The Raman signal of nucleic acid bases was slightly enlarged due to the surface-enhanced Raman scattering activity brought by the aggregation of silver atoms.Furthermore,the experiment results showed that the Raman spectra with high signal to noise ratio and with good repeatability could be obtained by this method and the Raman spectra of nucleic acid bases ob |
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| Publisher | 吉林大学分子酶学工程教育部重点实验室,长春,130012%吉林大学生命科学学院,长春,130012%吉林省长春市疾病预防控制中心,长春,130033%长春医学高等专科学校,长春,130013 |
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| SubjectTerms | 光还原反应 碱基检测 表面增强拉曼散射 银胶体 |
| Title | 基于在位光还原银胶法的表面增强拉曼散射技术检测核酸碱基 |
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