Optimal sequencing depth design for whole genome re-sequencing in pigs

• Published in: BMC bioinformatics Vol. 20; no. 1; pp. 556 - 12
• Main Authors: Jiang, Yifan, Jiang, Yao, Wang, Sheng, Zhang, Qin, Ding, Xiangdong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.11.2019; BioMed Central; BMC
• Subjects: Algorithms; Analysis; Animals; Base Sequence; Budgets; DNA sequencing; Genetics, Population; Genome coverage; Genomes; Genomics; High-Throughput Nucleotide Sequencing - methods; Pig; Polymorphism, Single Nucleotide - genetics; Principal Component Analysis; Reproducibility of Results; Sequencing depth; Swine; Swine - genetics; Whole Genome Sequencing - methods; Whole-genome sequencing; Iran; Whole-genome sequencing; Sequencing depth; Genome coverage; Pig

As whole-genome sequencing is becoming a routine technique, it is important to identify a cost-effective depth of sequencing for such studies. However, the relationship between sequencing depth and biological results from the aspects of whole-genome coverage, variant discovery power and the quality...