Actinobacteria from Termite Mounds Show Antiviral Activity against Bovine Viral Diarrhea Virus, a Surrogate Model for Hepatitis C Virus

• Published in: Evidence-based complementary and alternative medicine Vol. 2015; no. 2015; pp. 1 - 9
• Main Authors: Fantinatti-Garboggini, Fabiana, Arns, Clarice Weis, Afonso, Rafael Sanches, Uetanabaro, Ana Paula Trovatti, Kohn, Luciana Konecny, Barnabé, Ana Caroline de Souza, Martini, Matheus Cavalheiro, Bastos, Juliana Cristina Santiago, Rodrigues, Rodney Alexandre Ferreira, Padilla, Marina Aiello, Bomfim, Getúlio Freitas
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2015; Hindawi Limited
• Subjects: Actinobacteria; Antibiotics; Bacteria; Bovine viral diarrhea virus; Colleges & universities; Deoxyribonucleic acid; Diarrhea; DNA; Hepatitis; Hepatitis C virus; Isoptera; Metabolites; Natural products; Ribosomal DNA; Streptomyces; Termites
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2015/745754

Extracts from termite-associated bacteria were evaluated for in vitro antiviral activity against bovine viral diarrhea virus (BVDV). Two bacterial strains were identified as active, with percentages of inhibition (IP) equal to 98%. Both strains were subjected to functional analysis via the addition of virus and extract at different time points in cell culture; the results showed that they were effective as posttreatments. Moreover, we performed MTT colorimetric assays to identify the CC50, IC50, and SI values of these strains, and strain CDPA27 was considered the most promising. In parallel, the isolates were identified as Streptomyces through 16S rRNA gene sequencing analysis. Specifically, CDPA27 was identified as S. chartreusis. The CDPA27 extract was fractionated on a C18-E SPE cartridge, and the fractions were reevaluated. A 100% methanol fraction was identified to contain the compound(s) responsible for antiviral activity, which had an SI of 262.41. GC-MS analysis showed that this activity was likely associated with the compound(s) that had a peak retention time of 5 min. Taken together, the results of the present study provide new information for antiviral research using natural sources, demonstrate the antiviral potential of Streptomyces chartreusis compounds isolated from termite mounds against BVDV, and lay the foundation for further studies on the treatment of HCV infection.