Deficiency and absence of endogenous isoprene in adults, disqualified its putative origin

• Published in: Heliyon Vol. 7; no. 1; p. e05922
• Main Authors: Sukul, Pritam, Richter, Anna, Schubert, Jochen K., Miekisch, Wolfram
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2021; Elsevier
• Subjects: biomarkers; biosynthesis; Breath isoprene; breathing; Breathomics; childbirth; cholesterol; Cholesterol biosynthesis; Disease biomarker; gene expression; hemodynamics; humans; isoprene; mass spectrometry; menstrual cycle; Metabolic disorders; metabolites; metabolomics; Mevalonate pathway; phenotype; pregnancy; PTR-MS; Volatile metabolomics; Disease biomarker; Cholesterol biosynthesis; Mevalonate pathway; Breath isoprene; PTR-MS; Volatile metabolomics; Breathomics; Metabolic disorders
• DOI: 10.1016/j.heliyon.2021.e05922
• ISSN: 2405-8440

Isoprene (C5H8) is a clinically important breath metabolite. Although, hundreds of studies have reported differential expressions in isoprene exhalation as breath biomarker for diverse diseases, the substance couldn't enter to clinical practice as diagnostic marker. Moreover, many experimental/basic observations upon breath isoprene remained unrelated to the corresponding pathophysiological effects on its putative metabolic origin (i.e. mevalonate pathway). Here, we investigated the fundamental reason that hindered the rational interpretation and translation of this marker from basic to clinical science. Via high-resolution mass-spectrometry based breathomics in 1026 human subjects, we discovered adults with significant deficiency (order of magnitude lower than the normal) and complete absence of breath isoprene. We prospectively applied real-time breathomics, quantitative gene expression analysis of the mevalonate pathway enzymes, lipid-profiling and hemodynamic monitoring on those isoprene deficient subjects and controls. Additionally, the subject with absence of isoprene was followed up throughout different phases of her womanhood. In contrast to convention, we witnessed that adults can live healthy without exhaling isoprene or with significant deficiency. This rare phenotype represents a recessive inheritance. Despite physio-metabolic changes during menstrual cycle (that is known to profoundly affect isoprene exhalation) and profoundly increased plasma cholesterol during pregnancy and after childbirth, isoprene remained absent. All genes of mevalonate pathway enzymes were normally expressed in all participants, without any down-regulation or compensatory up-regulation. Absence/deficiency of isoprene despite normal lipid profiles and no mevalonate pathway malfunction disqualifies the long-believed metabolic origin of isoprene from cholesterol biosynthesis. Thus, clinical translation of breath isoprene expressions should not be generally attributed to corresponding pathophysiological effects onto mevalonate/cholesterol pathway. Our finding has refined and optimized the clinical interpretation of isoprene as biomarker in volatile metabolomics and breathomics. Future studies will address the correct metabolic origin of isoprene to imply this important marker to routine practice. Breath isoprene, Disease biomarker, Cholesterol biosynthesis, Mevalonate pathway, PTR-MS, Metabolic disorders, Volatile metabolomics, Breathomics.