The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies - Brooks, J Paul; Edwards, David J; Harwich, Michael D; Rivera, Maria C; Fettweis, Jennifer M; Serrano, Myrna G; Reris, Robert A; Sheth, Nihar U; Huang, Bernice; Girerd, Philippe; Strauss, Jerome F; Jefferson, Kimberly K; et al. (BMC microbiology, 2015)
Keywords: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bias; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Female; Genes, rRNA; High-Throughput Nucleotide Sequencing - standards; Humans; Metagenomics - instrumentation; Metagenomics - methods; Metagenomics - standards; Methodology; Microbial Consortia - genetics; Microbiota - genetics; Models, Biological; Phylogeny; Polymerase Chain Reaction - standards; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - isolation & purification; Specimen Handling - standards; Vagina - microbiology