Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood

• Published in: EBioMedicine Vol. 43; pp. 114 - 126
• Main Authors: Notarangelo, Michela, Zucal, Chiara, Modelska, Angelika, Pesce, Isabella, Scarduelli, Giorgina, Potrich, Cristina, Lunelli, Lorenzo, Pederzolli, Cecilia, Pavan, Paola, la Marca, Giancarlo, Pasini, Luigi, Ulivi, Paola, Beltran, Himisha, Demichelis, Francesca, Provenzani, Alessandro, Quattrone, Alessandro, D'Agostino, Vito G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2019; Elsevier
• Subjects: Advanced Basic Science; Alpha; Biomarkers; Biomarkers, Tumor - blood; Cancer; Case-Control Studies; Cell Line, Tumor; Droplet PCR; Extracellular vesicles; Extracellular Vesicles - metabolism; Extracellular Vesicles - ultrastructure; Flow Cytometry; Humans; Internal Medicine; Liquid biopsy; Liquid Biopsy - methods; Liquid Biopsy - standards; Neoplasms - blood; Neoplasms - diagnosis; Neoplasms - genetics; Neoplasms - metabolism; Nickel; Polymerase Chain Reaction; Research paper; Sensitivity and Specificity; Ultracentrifugation; Biomarkers; Extracellular vesicles; Alpha; Liquid biopsy; Nickel; Droplet PCR; Cancer

Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice. We designed the ni...