Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood
Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice. We designed the ni...
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| Published in | EBioMedicine Vol. 43; pp. 114 - 126 |
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| Main Authors | , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Netherlands
Elsevier B.V
01.05.2019
Elsevier |
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| Abstract | Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice.
We designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility.
From plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 1010/ml) and large EV (~560 nm of mean diameter; ~5 × 108/ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endothelial cell markers that were also used in spike-in experiments using EVs from tumor cell lines. In retrospective studies, we detected picomolar concentrations of prostate-specific membrane antigen (PSMA) in fractions of EVs isolated from the plasma of prostate cancer patients, discriminating them from control subjects. Directly from oil-encapsulated EVs for digital PCR, we identified somatic BRAF and KRAS mutations circulating in the plasma of metastatic colorectal cancer (CRC) patients, matching 100% of concordance with tissue diagnostics. Importantly, with higher sensitivity and specificity compared with immuno-isolated EVs, we revealed additional somatic alterations in 7% of wild-type CRC cases that were subsequently validated by further inspections in the matched tissue biopsies.
We propose NBI-combined approaches as simple, fast, and robust strategies to probe the tumor heterogeneity and contribute to the development of EV-based liquid biopsy studies.
Associazione Italiana per la Ricerca sul Cancro (AIRC), Fondazione Cassa di Risparmio Trento e Rovereto (CARITRO), and the Italian Ministero Istruzione, Università e Ricerca (Miur). |
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| AbstractList | Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice.BACKGROUNDExtracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice.We designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility.METHODSWe designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility.From plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 1010/ml) and large EV (~560 nm of mean diameter; ~5 × 108/ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endothelial cell markers that were also used in spike-in experiments using EVs from tumor cell lines. In retrospective studies, we detected picomolar concentrations of prostate-specific membrane antigen (PSMA) in fractions of EVs isolated from the plasma of prostate cancer patients, discriminating them from control subjects. Directly from oil-encapsulated EVs for digital PCR, we identified somatic BRAF and KRAS mutations circulating in the plasma of metastatic colorectal cancer (CRC) patients, matching 100% of concordance with tissue diagnostics. Importantly, with higher sensitivity and specificity compared with immuno-isolated EVs, we revealed additional somatic alterations in 7% of wild-type CRC cases that were subsequently validated by further inspections in the matched tissue biopsies.FINDINGSFrom plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 1010/ml) and large EV (~560 nm of mean diameter; ~5 × 108/ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endothelial cell markers that were also used in spike-in experiments using EVs from tumor cell lines. In retrospective studies, we detected picomolar concentrations of prostate-specific membrane antigen (PSMA) in fractions of EVs isolated from the plasma of prostate cancer patients, discriminating them from control subjects. Directly from oil-encapsulated EVs for digital PCR, we identified somatic BRAF and KRAS mutations circulating in the plasma of metastatic colorectal cancer (CRC) patients, matching 100% of concordance with tissue diagnostics. Importantly, with higher sensitivity and specificity compared with immuno-isolated EVs, we revealed additional somatic alterations in 7% of wild-type CRC cases that were subsequently validated by further inspections in the matched tissue biopsies.We propose NBI-combined approaches as simple, fast, and robust strategies to probe the tumor heterogeneity and contribute to the development of EV-based liquid biopsy studies. FUND: Associazione Italiana per la Ricerca sul Cancro (AIRC), Fondazione Cassa di Risparmio Trento e Rovereto (CARITRO), and the Italian Ministero Istruzione, Università e Ricerca (Miur).INTERPRETATIONWe propose NBI-combined approaches as simple, fast, and robust strategies to probe the tumor heterogeneity and contribute to the development of EV-based liquid biopsy studies. FUND: Associazione Italiana per la Ricerca sul Cancro (AIRC), Fondazione Cassa di Risparmio Trento e Rovereto (CARITRO), and the Italian Ministero Istruzione, Università e Ricerca (Miur). AbstractBackgroundExtracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice. MethodsWe designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility. FindingsFrom plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 10 10/ml) and large EV (~560 nm of mean diameter; ~5 × 10 8/ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endothelial cell markers that were also used in spike-in experiments using EVs from tumor cell lines. In retrospective studies, we detected picomolar concentrations of prostate-specific membrane antigen (PSMA) in fractions of EVs isolated from the plasma of prostate cancer patients, discriminating them from control subjects. Directly from oil-encapsulated EVs for digital PCR, we identified somatic BRAF and KRAS mutations circulating in the plasma of metastatic colorectal cancer (CRC) patients, matching 100% of concordance with tissue diagnostics. Importantly, with higher sensitivity and specificity compared with immuno-isolated EVs, we revealed additional somatic alterations in 7% of wild-type CRC cases that were subsequently validated by further inspections in the matched tissue biopsies. InterpretationWe propose NBI-combined approaches as simple, fast, and robust strategies to probe the tumor heterogeneity and contribute to the development of EV-based liquid biopsy studies. FundAssociazione Italiana per la Ricerca sul Cancro (AIRC), Fondazione Cassa di Risparmio Trento e Rovereto (CARITRO), and the Italian Ministero Istruzione, Università e Ricerca (Miur). Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice. We designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility. From plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 1010/ml) and large EV (~560 nm of mean diameter; ~5 × 108/ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endothelial cell markers that were also used in spike-in experiments using EVs from tumor cell lines. In retrospective studies, we detected picomolar concentrations of prostate-specific membrane antigen (PSMA) in fractions of EVs isolated from the plasma of prostate cancer patients, discriminating them from control subjects. Directly from oil-encapsulated EVs for digital PCR, we identified somatic BRAF and KRAS mutations circulating in the plasma of metastatic colorectal cancer (CRC) patients, matching 100% of concordance with tissue diagnostics. Importantly, with higher sensitivity and specificity compared with immuno-isolated EVs, we revealed additional somatic alterations in 7% of wild-type CRC cases that were subsequently validated by further inspections in the matched tissue biopsies. We propose NBI-combined approaches as simple, fast, and robust strategies to probe the tumor heterogeneity and contribute to the development of EV-based liquid biopsy studies. Associazione Italiana per la Ricerca sul Cancro (AIRC), Fondazione Cassa di Risparmio Trento e Rovereto (CARITRO), and the Italian Ministero Istruzione, Università e Ricerca (Miur). Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice. We designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility. From plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 10 /ml) and large EV (~560 nm of mean diameter; ~5 × 10 /ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endothelial cell markers that were also used in spike-in experiments using EVs from tumor cell lines. In retrospective studies, we detected picomolar concentrations of prostate-specific membrane antigen (PSMA) in fractions of EVs isolated from the plasma of prostate cancer patients, discriminating them from control subjects. Directly from oil-encapsulated EVs for digital PCR, we identified somatic BRAF and KRAS mutations circulating in the plasma of metastatic colorectal cancer (CRC) patients, matching 100% of concordance with tissue diagnostics. Importantly, with higher sensitivity and specificity compared with immuno-isolated EVs, we revealed additional somatic alterations in 7% of wild-type CRC cases that were subsequently validated by further inspections in the matched tissue biopsies. We propose NBI-combined approaches as simple, fast, and robust strategies to probe the tumor heterogeneity and contribute to the development of EV-based liquid biopsy studies. FUND: Associazione Italiana per la Ricerca sul Cancro (AIRC), Fondazione Cassa di Risparmio Trento e Rovereto (CARITRO), and the Italian Ministero Istruzione, Università e Ricerca (Miur). |
| Author | Scarduelli, Giorgina Ulivi, Paola Provenzani, Alessandro Modelska, Angelika Demichelis, Francesca la Marca, Giancarlo Zucal, Chiara Pederzolli, Cecilia D'Agostino, Vito G. Potrich, Cristina Lunelli, Lorenzo Beltran, Himisha Notarangelo, Michela Pavan, Paola Quattrone, Alessandro Pesce, Isabella Pasini, Luigi |
| AuthorAffiliation | e Immunohematology and Cell Factory Unit, Meyer Children's University Hospital, Viale Pieraccini 24, Florence 50139, Italy f Department of Experimental and Clinical Biomedical Sciences, Centro di Eccellenza Denothe, Aou Meyer University of Florence, Viale Pieraccini 6, 50139, Italy h Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA, USA a Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy c Advanced Imaging Core Facility (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy g Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Via Piero Maroncelli 40, Meldola 47014, Italy b Cell Analysis and Separation Core Facility (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy d Fondazione Bruno Kessler (FBK), Laboratory of Biomolecular Sequence and Structure Analysis for Health, Trento, Via Sommarive 14, Trento 38123, Italy |
| AuthorAffiliation_xml | – name: b Cell Analysis and Separation Core Facility (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – name: d Fondazione Bruno Kessler (FBK), Laboratory of Biomolecular Sequence and Structure Analysis for Health, Trento, Via Sommarive 14, Trento 38123, Italy – name: f Department of Experimental and Clinical Biomedical Sciences, Centro di Eccellenza Denothe, Aou Meyer University of Florence, Viale Pieraccini 6, 50139, Italy – name: e Immunohematology and Cell Factory Unit, Meyer Children's University Hospital, Viale Pieraccini 24, Florence 50139, Italy – name: c Advanced Imaging Core Facility (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – name: a Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – name: h Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA, USA – name: g Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Via Piero Maroncelli 40, Meldola 47014, Italy |
| Author_xml | – sequence: 1 givenname: Michela surname: Notarangelo fullname: Notarangelo, Michela organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 2 givenname: Chiara surname: Zucal fullname: Zucal, Chiara organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 3 givenname: Angelika surname: Modelska fullname: Modelska, Angelika organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 4 givenname: Isabella surname: Pesce fullname: Pesce, Isabella organization: Cell Analysis and Separation Core Facility (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 5 givenname: Giorgina surname: Scarduelli fullname: Scarduelli, Giorgina organization: Advanced Imaging Core Facility (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 6 givenname: Cristina surname: Potrich fullname: Potrich, Cristina organization: Fondazione Bruno Kessler (FBK), Laboratory of Biomolecular Sequence and Structure Analysis for Health, Trento, Via Sommarive 14, Trento 38123, Italy – sequence: 7 givenname: Lorenzo surname: Lunelli fullname: Lunelli, Lorenzo organization: Fondazione Bruno Kessler (FBK), Laboratory of Biomolecular Sequence and Structure Analysis for Health, Trento, Via Sommarive 14, Trento 38123, Italy – sequence: 8 givenname: Cecilia surname: Pederzolli fullname: Pederzolli, Cecilia organization: Fondazione Bruno Kessler (FBK), Laboratory of Biomolecular Sequence and Structure Analysis for Health, Trento, Via Sommarive 14, Trento 38123, Italy – sequence: 9 givenname: Paola surname: Pavan fullname: Pavan, Paola organization: Immunohematology and Cell Factory Unit, Meyer Children's University Hospital, Viale Pieraccini 24, Florence 50139, Italy – sequence: 10 givenname: Giancarlo surname: la Marca fullname: la Marca, Giancarlo organization: Department of Experimental and Clinical Biomedical Sciences, Centro di Eccellenza Denothe, Aou Meyer University of Florence, Viale Pieraccini 6, 50139, Italy – sequence: 11 givenname: Luigi surname: Pasini fullname: Pasini, Luigi organization: Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Via Piero Maroncelli 40, Meldola 47014, Italy – sequence: 12 givenname: Paola surname: Ulivi fullname: Ulivi, Paola organization: Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Via Piero Maroncelli 40, Meldola 47014, Italy – sequence: 13 givenname: Himisha surname: Beltran fullname: Beltran, Himisha email: himisha_beltran@dfci.harvard.edu organization: Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA, USA – sequence: 14 givenname: Francesca surname: Demichelis fullname: Demichelis, Francesca organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 15 givenname: Alessandro surname: Provenzani fullname: Provenzani, Alessandro organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 16 givenname: Alessandro surname: Quattrone fullname: Quattrone, Alessandro organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy – sequence: 17 givenname: Vito G. orcidid: 0000-0003-3379-2254 surname: D'Agostino fullname: D'Agostino, Vito G. email: vito.dagostino@unitn.it organization: Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Via Sommarive 9, Trento 38123, Italy |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31047861$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.3402/jev.v4.28533 10.1080/09537104.2016.1212002 10.1146/annurev-cellbio-101512-122326 10.1038/mtna.2013.28 10.1073/pnas.1522297113 10.1016/S1470-2045(01)00560-5 10.1007/s00109-013-1020-6 10.1039/C8LC01193A 10.2144/000114575 10.1073/pnas.90.23.11122 10.1002/cyto.a.22892 10.1080/15384047.2015.1113356 10.1186/s12885-018-5138-3 10.1038/s41598-017-14981-x 10.1021/acsnano.7b07782 10.1080/20013078.2018.1505403 10.1016/j.bbalip.2013.04.011 10.1242/jcs.128868 10.1083/jcb.201211138 10.1038/srep31175 10.1006/scel.1993.1049 10.1038/srep33935 10.1098/rsta.2010.0175 10.1016/j.cell.2016.01.043 10.1038/nmeth.4185 10.1515/hsz-2013-0141 10.3390/membranes4030356 10.1038/s41551-017-0058 10.3892/ijmm.2016.2759 10.1002/0471143030.cb0322s30 10.1016/0005-2736(91)90295-J 10.1111/j.1365-2141.1967.tb08741.x 10.1016/j.ajpath.2012.07.030 10.1038/s41598-018-24163-y 10.1007/s12185-017-2180-x 10.3402/jev.v2i0.20424 10.1038/ncb1725 10.3402/jev.v5.32570 10.3402/jev.v5.31803 10.1080/15384047.2017.1281499 10.1073/pnas.1221899110 10.3402/jev.v4.29260 10.1053/j.gastro.2014.09.038 10.1038/s41598-017-01420-0 10.1016/j.mam.2017.11.012 10.1186/s12967-015-0619-5 10.1016/j.ejps.2016.09.008 10.1038/s41551-016-0021 10.1016/j.talanta.2018.10.016 10.1093/annonc/mdx004 10.1371/journal.pone.0072426 10.3402/jev.v3.24692 10.3402/jev.v5.31242 10.3402/jev.v4.26316 10.1016/j.jtho.2018.05.030 10.3402/jev.v3.25011 10.1016/j.bbagen.2016.07.028 10.1007/978-1-4939-0856-1_11 10.1007/s10571-016-0366-z 10.1016/S1452-3981(23)16554-0 10.1021/acsnano.5b08015 10.1074/jbc.M115.680389 10.1080/09537104.2016.1265924 10.1038/s41416-018-0115-9 10.3402/jev.v4.27066 10.1021/acs.langmuir.6b02493 10.3402/jev.v3.23111 10.1038/nrm.2017.125 10.3402/jev.v5.25355 10.1038/ncomms4591 |
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| References | Ulivi, Scarpi, Passardi, Marisi, Calistri, Zoli (bb0320) 2015; 13 Lee, Hur, Kim, Kim, Choi, Lee (bb0075) 2018 Dec 10; 18 Wan, Cheng, Liu, Hao, Nisic, Zhu (bb0120) 2017 Kosanović, Milutinović, Goč, Mitić, Janković (bb0135) 2017; 63 Kaszuba, Corbett, Watson, Jones (bb0155) 2010; 368 Haraszti, Didiot, Sapp, Leszyk, Shaffer, Rockwell (bb0195) 2016 Jan 24; 5 Colombo, Moita, van Niel, Kowal, Vigneron, Benaroch (bb0210) 2013 Dec 15; 126 Deregibus, Figliolini, D'Antico, Manzini, Pasquino, De Lena (bb0130) 2016 Nov 29; 38 Heath, Grant, De Oliveira, Rowlinson, Osteikoetxea, Dekker (bb0140) 2018 Dec 10; 8 Kahlert, Kalluri (bb0045) 2013; 91 Di Vizio, Morello, Dudley, Schow, Adam, Morley (bb0170) 2012 Nov; 181 El-Deiry, Vijayvergia, Xiu, Scicchitano, Lim, Yee (bb0365) 2015; 16 Raposo, Stoorvogel (bb0005) 2013 Feb 18; 200 Marisi, Scarpi, Passardi, Nanni, Ragazzini, Valgiusti (bb0325) 2017; 7 Gardiner, Di, Sahoo, Théry, Witwer, Wauben (bb0100) 2016 Oct 31; 5 Oliveira-Rodríguez, López-Cobo, Reyburn, Costa-García, López-Martín, Yáñez-Mó (bb0125) 2016; 5 Phipps, Limburg, Baron, Burnett-Hartman, Weisenberger, Laird (bb0355) 2015; 148 Vogel, Coumans, Maltesen, Böing, Bonnington, Broekman (bb0205) 2016 Jan 24; 5 Valkonen, van der Pol, Böing, Yuana, Yliperttula, Nieuwland (bb0250) 2017 Feb 15; 98 Vagida, Arakelyan, Lebedeva, Grivel, Shpektor, Vasilieva (bb0255) 2017 Mar 17; 28 Momen-Heravi, Balaj, Alian, Mantel, Halleck, Trachtenberg (bb0105) 2013 Jan 1; 394 Tatischeff (bb0305) 2016 Yoshioka, Kosaka, Konishi, Ohta, Okamoto, Sonoda (bb0285) 2014; 5 Kim, Nishida, An, Shetty, Bartosh, Prockop (bb0345) 2016 Jan 5; 113 Antonyak, Cerione (bb0010) 2014; 1165 Nomura (bb0235) 2017 Apr 27; 105 Smith, Lee, Rojalin, Carney, Hazari, Knudson (bb0290) 2015; 4 Yoshioka, Konishi, Kosaka, Katsuda, Kato, Ochiya (bb0300) 2013; 2 Wisgrill, Lamm, Hartmann, Preißing, Dragosits, Bee (bb0245) 2016 Jul; 89 D'Agostino, Adami, Provenzani (bb0180) 2013 Jan; 8 Cardi, Mastrangelo, Berd (bb0275) 1989 Dec 1; 49 van Niel, D'Angelo, Raposo (bb0025) 2018; 19 Llorente, Skotland, Sylvänne, Kauhanen, Róg, Orłowski (bb0190) 2013 Jul; 1831 Allenson, Castillo, San Lucas, Scelo, Kim, Bernard (bb0085) 2017; 28 Předota, Machesky, Wesolowski (bb0145) 2016; 32 Abels, Breakefield (bb0230) 2016 Apr 6; 36 Ramakrishnaiah, Thumann, Fofana, Habersetzer, Pan, de Ruiter (bb0335) 2013; 110 Van Deun, Mestdagh, Sormunen, Cocquyt, Vermaelen, Vandesompele (bb0175) 2014 Sep 18; 3 Woodford-Thomas, Thomas (bb0270) 1993 Dec; 4 Belov, Matic, Hallal, Best, Mulligan, Christopherson (bb0280) 2016; 5 MacDonald, MacDonald, Menco, Takeshita, Subbarao, Hu (bb0200) 1991; 1061 Yang, Che, Kurywchak, Tavormina, Gansmo, Correa de Sampaio (bb0080) 2017 Mar 4; 18 Melki, Tessandier, Zufferey, Boilard (bb0260) 2017; 28 Ortega, Piguillem, Messina, Tortella, Rubilar, Jiménez Castillo (bb0070) 2019 Mar 1; 194 Al-Nedawi, Meehan, Micallef, Lhotak, May, Guha (bb0015) 2008; 10 Wei, Batagov, Carter, Krichevsky (bb0225) 2016 Aug 9; 6 Chen, Balaj, Liau, Samuels, Kotsopoulos, Maguire (bb0310) 2013; 2 Salgin, Salgin, Bahadir (bb0150) 2012; 7 Colombo, Raposo, Thery (bb0020) 2014 Oct 11; 30 EV-TRACK Consortium J, Van Deun, Mestdagh, Agostinis, Akay, Anand (bb0095) 2017 Feb 28; 14 Thomsen, Skovlund, Sorbye, Bolstad, Nustad, Glimelius (bb0360) 2018 Nakai, Yoshida, Diez, Miyatake, Nishibu, Imawaka (bb0340) 2016 Sep 23; 6 Tian, Ma, Gong, Su, Zhu, Zhang (bb0330) 2018; 12 Yuana, Böing, Grootemaat, van der Pol, Hau, Cizmar (bb0220) 2015; 4 Nogués, Benito-Martin, Hergueta-Redondo, Peinado (bb0040) 2018; 60 McDonald, Reep, Lapetina, Molina y Vedia (bb0315) 1993 Dec 1; 90 Xu, Liu, Ahmed, Loppnau, Schapira, Min (bb0185) 2015 Oct 9; 290 Rupert, Claudio, Lässer, Bally (bb0165) 2017 Jan; 1861 Srinivas, Kramer, Srivastava (bb0350) 2001; 2 Rolfo, Mack, Scagliotti, Baas, Barlesi, Bivona (bb0370) 2018; 13 Vagner, Spinelli, Minciacchi, Balaj, Zandian, Conley (bb0090) 2018 Dec 7; 7 Fais, O'Driscoll, Borras, Buzas, Camussi, Cappello (bb0060) 2016; 10 Aatonen, Ohman, Nyman, Laitinen, Grönholm, Siljander (bb0265) 2014; 3 Liang, Liu, Fan, Sun, Liu, Lyon (bb0115) 2017 Meister, Tikkanen (bb0215) 2014 Jul 11; 4 Cvjetkovic, Lötvall, Lässer (bb0110) 2014 Jan 24; 3 Wiklander, Nordin, O'Loughlin, Gustafsson, Corso, Mäger (bb0055) 2015; 4 Chen, Xu, Wang, Liu, Yang, Zhu (bb0065) 2019; 19 Vogel, Pal, Jambhrunkar, Patel, Thakur, Reátegui (bb0160) 2017; 7 Wolf (bb0240) 1967; 13 Théry, Amigorena, Raposo, Clayton (bb0030) 2006 Apr Yáñez-Mó, Siljander, Andreu, Zavec, Borràs, Buzas (bb0050) 2015; 4 Jeppesen, Hvam, Primdahl-Bengtson, Boysen, Whitehead, Dyrskj�t (bb0295) 2014; 3 Tkach, Théry (bb0035) 2016; 164 Al-Nedawi (10.1016/j.ebiom.2019.04.039_bb0015) 2008; 10 Ramakrishnaiah (10.1016/j.ebiom.2019.04.039_bb0335) 2013; 110 Ulivi (10.1016/j.ebiom.2019.04.039_bb0320) 2015; 13 Yáñez-Mó (10.1016/j.ebiom.2019.04.039_bb0050) 2015; 4 Di Vizio (10.1016/j.ebiom.2019.04.039_bb0170) 2012; 181 Vogel (10.1016/j.ebiom.2019.04.039_bb0160) 2017; 7 Yang (10.1016/j.ebiom.2019.04.039_bb0080) 2017; 18 Wiklander (10.1016/j.ebiom.2019.04.039_bb0055) 2015; 4 Allenson (10.1016/j.ebiom.2019.04.039_bb0085) 2017; 28 Gardiner (10.1016/j.ebiom.2019.04.039_bb0100) 2016; 5 Yuana (10.1016/j.ebiom.2019.04.039_bb0220) 2015; 4 Nogués (10.1016/j.ebiom.2019.04.039_bb0040) 2018; 60 Chen (10.1016/j.ebiom.2019.04.039_bb0310) 2013; 2 Phipps (10.1016/j.ebiom.2019.04.039_bb0355) 2015; 148 Nomura (10.1016/j.ebiom.2019.04.039_bb0235) 2017; 105 Meister (10.1016/j.ebiom.2019.04.039_bb0215) 2014; 4 McDonald (10.1016/j.ebiom.2019.04.039_bb0315) 1993; 90 Rupert (10.1016/j.ebiom.2019.04.039_bb0165) 2017; 1861 Melki (10.1016/j.ebiom.2019.04.039_bb0260) 2017; 28 Srinivas (10.1016/j.ebiom.2019.04.039_bb0350) 2001; 2 Kahlert (10.1016/j.ebiom.2019.04.039_bb0045) 2013; 91 Thomsen (10.1016/j.ebiom.2019.04.039_bb0360) 2018 Cardi (10.1016/j.ebiom.2019.04.039_bb0275) 1989; 49 Fais (10.1016/j.ebiom.2019.04.039_bb0060) 2016; 10 Woodford-Thomas (10.1016/j.ebiom.2019.04.039_bb0270) 1993; 4 Antonyak (10.1016/j.ebiom.2019.04.039_bb0010) 2014; 1165 D'Agostino (10.1016/j.ebiom.2019.04.039_bb0180) 2013; 8 Deregibus (10.1016/j.ebiom.2019.04.039_bb0130) 2016; 38 Valkonen (10.1016/j.ebiom.2019.04.039_bb0250) 2017; 98 Tkach (10.1016/j.ebiom.2019.04.039_bb0035) 2016; 164 Nakai (10.1016/j.ebiom.2019.04.039_bb0340) 2016; 6 van Niel (10.1016/j.ebiom.2019.04.039_bb0025) 2018; 19 Wan (10.1016/j.ebiom.2019.04.039_bb0120) 2017 Kaszuba (10.1016/j.ebiom.2019.04.039_bb0155) 2010; 368 Rolfo (10.1016/j.ebiom.2019.04.039_bb0370) 2018; 13 Ortega (10.1016/j.ebiom.2019.04.039_bb0070) 2019; 194 Liang (10.1016/j.ebiom.2019.04.039_bb0115) 2017 Salgin (10.1016/j.ebiom.2019.04.039_bb0150) 2012; 7 Raposo (10.1016/j.ebiom.2019.04.039_bb0005) 2013; 200 Marisi (10.1016/j.ebiom.2019.04.039_bb0325) 2017; 7 EV-TRACK Consortium J (10.1016/j.ebiom.2019.04.039_bb0095) 2017; 14 Kosanović (10.1016/j.ebiom.2019.04.039_bb0135) 2017; 63 Llorente (10.1016/j.ebiom.2019.04.039_bb0190) 2013; 1831 Lee (10.1016/j.ebiom.2019.04.039_bb0075) 2018; 18 Wolf (10.1016/j.ebiom.2019.04.039_bb0240) 1967; 13 Tatischeff (10.1016/j.ebiom.2019.04.039_bb0305) 2016 Xu (10.1016/j.ebiom.2019.04.039_bb0185) 2015; 290 Abels (10.1016/j.ebiom.2019.04.039_bb0230) 2016; 36 Jeppesen (10.1016/j.ebiom.2019.04.039_bb0295) 2014; 3 Kim (10.1016/j.ebiom.2019.04.039_bb0345) 2016; 113 Chen (10.1016/j.ebiom.2019.04.039_bb0065) 2019; 19 Momen-Heravi (10.1016/j.ebiom.2019.04.039_bb0105) 2013; 394 Tian (10.1016/j.ebiom.2019.04.039_bb0330) 2018; 12 MacDonald (10.1016/j.ebiom.2019.04.039_bb0200) 1991; 1061 Vagner (10.1016/j.ebiom.2019.04.039_bb0090) 2018; 7 Colombo (10.1016/j.ebiom.2019.04.039_bb0020) 2014; 30 Haraszti (10.1016/j.ebiom.2019.04.039_bb0195) 2016; 5 Vagida (10.1016/j.ebiom.2019.04.039_bb0255) 2017; 28 Yoshioka (10.1016/j.ebiom.2019.04.039_bb0300) 2013; 2 Aatonen (10.1016/j.ebiom.2019.04.039_bb0265) 2014; 3 Smith (10.1016/j.ebiom.2019.04.039_bb0290) 2015; 4 Belov (10.1016/j.ebiom.2019.04.039_bb0280) 2016; 5 Yoshioka (10.1016/j.ebiom.2019.04.039_bb0285) 2014; 5 Wei (10.1016/j.ebiom.2019.04.039_bb0225) 2016; 6 Oliveira-Rodríguez (10.1016/j.ebiom.2019.04.039_bb0125) 2016; 5 Colombo (10.1016/j.ebiom.2019.04.039_bb0210) 2013; 126 Cvjetkovic (10.1016/j.ebiom.2019.04.039_bb0110) 2014; 3 Předota (10.1016/j.ebiom.2019.04.039_bb0145) 2016; 32 Wisgrill (10.1016/j.ebiom.2019.04.039_bb0245) 2016; 89 Théry (10.1016/j.ebiom.2019.04.039_bb0030) 2006 El-Deiry (10.1016/j.ebiom.2019.04.039_bb0365) 2015; 16 Van Deun (10.1016/j.ebiom.2019.04.039_bb0175) 2014; 3 Vogel (10.1016/j.ebiom.2019.04.039_bb0205) 2016; 5 Heath (10.1016/j.ebiom.2019.04.039_bb0140) 2018; 8 31101598 - EBioMedicine. 2019 Jun;44:14-15. doi: 10.1016/j.ebiom.2019.05.021 |
| References_xml | – volume: 164 start-page: 1226 year: 2016 end-page: 1232 ident: bb0035 article-title: Communication by extracellular vesicles: where we are and where we need to go publication-title: Cell – volume: 194 start-page: 243 year: 2019 Mar 1 end-page: 252 ident: bb0070 article-title: EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform publication-title: Talanta – volume: 19 start-page: 213 year: 2018 end-page: 228 ident: bb0025 article-title: Shedding light on the cell biology of extracellular vesicles publication-title: Nat Rev Mol Cell Biol – volume: 4 start-page: 29260 year: 2015 ident: bb0220 article-title: Handling and storage of human body fluids for analysis of extracellular vesicles publication-title: J Extracell Vesicles – volume: 3 start-page: 25011 year: 2014 ident: bb0295 article-title: Comparative analysis of discrete exosome fractions obtained by differential centrifugation publication-title: J Extracell Vesicles – volume: 4 start-page: 27066 year: 2015 ident: bb0050 article-title: Biological properties of extracellular vesicles and their physiological functions publication-title: J Extracell Vesicles – volume: 105 start-page: 392 year: 2017 Apr 27 end-page: 405 ident: bb0235 article-title: Extracellular vesicles and blood diseases publication-title: Int J Hematol – volume: 89 start-page: 663 year: 2016 Jul end-page: 672 ident: bb0245 article-title: Peripheral blood microvesicles secretion is influenced by storage time, temperature, and anticoagulants publication-title: Cytom Part A – volume: 148 year: 2015 ident: bb0355 article-title: Association between molecular subtypes of colorectal cancer and patient survival publication-title: Gastroenterology – volume: 6 start-page: 33935 year: 2016 Sep 23 ident: bb0340 article-title: A novel affinity-based method for the isolation of highly purified extracellular vesicles publication-title: Sci Rep – volume: 6 start-page: 31175 year: 2016 Aug 9 ident: bb0225 article-title: Fetal bovine serum RNA interferes with the cell culture derived extracellular RNA publication-title: Sci Rep – volume: 14 start-page: 228 year: 2017 Feb 28 end-page: 232 ident: bb0095 article-title: EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research publication-title: Nat Methods – volume: 28 start-page: 741 year: 2017 end-page: 747 ident: bb0085 article-title: High prevalence of mutant KRAS in circulating exosome-derived DNA from early-stage pancreatic cancer patients publication-title: Ann Oncol – volume: 181 start-page: 1573 year: 2012 Nov end-page: 1584 ident: bb0170 article-title: Large oncosomes in human prostate cancer tissues and in the circulation of mice with metastatic disease publication-title: Am J Pathol – volume: 60 start-page: 15 year: 2018 end-page: 26 ident: bb0040 article-title: The influence of tumour-derived extracellular vesicles on local and distal metastatic dissemination publication-title: Mol Aspects Med – year: 2017 ident: bb0120 article-title: Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes publication-title: Nat Biomed Eng – volume: 63 start-page: 65 year: 2017 end-page: 71 ident: bb0135 article-title: Ion-exchange chromatography purification of extracellular vesicles publication-title: Biotechniques – volume: 8 start-page: 5730 year: 2018 Dec 10 ident: bb0140 article-title: Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography publication-title: Sci Rep – volume: 16 start-page: 1726 year: 2015 end-page: 1737 ident: bb0365 article-title: Molecular profiling of 6,892 colorectal cancer samples suggests different possible treatment options specific to metastatic sites publication-title: Cancer Biol Ther – year: 2006 Apr ident: bb0030 article-title: Isolation and characterization of exosomes from cell culture supernatants and biological fluids publication-title: Curr Protoc Cell Biol – volume: 1861 start-page: 3164 year: 2017 Jan end-page: 3179 ident: bb0165 article-title: Methods for the physical characterization and quantification of extracellular vesicles in biological samples publication-title: Biochim Biophys Acta – volume: 4 start-page: 28533 year: 2015 ident: bb0290 article-title: Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content publication-title: J Extracell Vesicles – volume: 7 start-page: 17479 year: 2017 ident: bb0160 article-title: High-resolution single particle zeta potential characterisation of biological nanoparticles using tunable resistive pulse sensing publication-title: Sci Rep – volume: 2 year: 2013 ident: bb0310 article-title: Beaming and droplet digital pcr analysis of mutant idh1 mrna in glioma patient serum and cerebrospinal fluid extracellular vesicles publication-title: Mol Ther Nucleic Acids – volume: 1165 start-page: 147 year: 2014 end-page: 173 ident: bb0010 article-title: Microvesicles as mediators of intercellular communication in cancer publication-title: Methods Mol Biol – volume: 30 start-page: 255 year: 2014 Oct 11 end-page: 289 ident: bb0020 article-title: Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles publication-title: Annu Rev Cell Dev Biol – volume: 290 start-page: 24902 year: 2015 Oct 9 end-page: 24913 ident: bb0185 article-title: Structural basis for the discriminative recognition of publication-title: J Biol Chem – year: 2016 ident: bb0305 article-title: Innovative approach of prostate cancer by the way of tumour cell-derived extracellular vesicles publication-title: J Extracell Vesicles – volume: 126 start-page: 5553 year: 2013 Dec 15 end-page: 5565 ident: bb0210 article-title: Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles publication-title: J Cell Sci – volume: 4 start-page: 356 year: 2014 Jul 11 end-page: 371 ident: bb0215 article-title: Endocytic trafficking of membrane-bound cargo: a flotillin point of view publication-title: Membranes (Basel) – volume: 7 start-page: 1293 year: 2017 ident: bb0325 article-title: Circulating VEGF and eNOS variations as predictors of outcome in metastatic colorectal cancer patients receiving bevacizumab publication-title: Sci Rep – volume: 3 year: 2014 Sep 18 ident: bb0175 article-title: The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling publication-title: J Extracell Vesicles – volume: 90 start-page: 11122 year: 1993 Dec 1 end-page: 11126 ident: bb0315 article-title: Glyceraldehyde-3-phosphate dehydrogenase is required for the transport of nitric oxide in platelets publication-title: Proc Natl Acad Sci U S A – volume: 2 start-page: 698 year: 2001 end-page: 704 ident: bb0350 article-title: Trends in biomarker research for cancer detection publication-title: Lancet Oncol – volume: 7 start-page: 1505403 year: 2018 Dec 7 ident: bb0090 article-title: Large extracellular vesicles carry most of the tumour DNA circulating in prostate cancer patient plasma publication-title: J Extracell Vesicles – volume: 18 start-page: 1236 year: 2018 Dec 10 ident: bb0075 article-title: Liquid biopsy using the supernatant of a pleural effusion for EGFR genotyping in pulmonary adenocarcinoma patients: a comparison between cell-free DNA and extracellular vesicle-derived DNA publication-title: BMC Cancer – volume: 28 start-page: 214 year: 2017 end-page: 221 ident: bb0260 article-title: Platelet microvesicles in health and disease publication-title: Platelets – year: 2017 ident: bb0115 article-title: Nanoplasmonic quantification of tumour-derived extracellular vesicles in plasma microsamples for diagnosis and treatment monitoring publication-title: Nat Biomed Eng – volume: 49 start-page: 6562 year: 1989 Dec 1 end-page: 6565 ident: bb0275 article-title: Depletion of T-cells with the CD4+CD45R+ phenotype in lymphocytes that infiltrate subcutaneous metastases of human melanoma publication-title: Cancer Res – volume: 10 start-page: 3886 year: 2016 end-page: 3899 ident: bb0060 article-title: Evidence-based clinical use of nanoscale extracellular vesicles in nanomedicine publication-title: ACS Nano – volume: 18 start-page: 158 year: 2017 Mar 4 end-page: 165 ident: bb0080 article-title: Detection of mutant KRAS and TP53 DNA in circulating exosomes from healthy individuals and patients with pancreatic cancer publication-title: Cancer Biol Ther – volume: 5 start-page: 25355 year: 2016 ident: bb0280 article-title: Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples publication-title: J Extracell Vesicles – volume: 19 start-page: 432 year: 2019 end-page: 443 ident: bb0065 article-title: Rapid and efficient isolation and detection of extracellular vesicles from plasma for lung cancer diagnosis publication-title: Lab Chip – volume: 12 start-page: 671 year: 2018 end-page: 680 ident: bb0330 article-title: Protein profiling and sizing of extracellular vesicles from colorectal cancer patients via flow cytometry publication-title: ACS Nano – volume: 5 start-page: 3591 year: 2014 ident: bb0285 article-title: Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen publication-title: Nat Commun – volume: 13 start-page: 1248 year: 2018 end-page: 1268 ident: bb0370 article-title: IASLC statement paper: liquid biopsy for advanced non-small cell lung cancer (NSCLC) publication-title: J Thorac Oncol – volume: 5 year: 2016 Oct 31 ident: bb0100 article-title: Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey publication-title: J Extracell Vesicles – volume: 7 start-page: 12404 year: 2012 end-page: 12414 ident: bb0150 article-title: Zeta potentials and isoelectric points of biomolecules: the effects of ion types and ionic strengths publication-title: Int J Electrochem Sci – volume: 3 year: 2014 ident: bb0265 article-title: Isolation and characterization of platelet-derived extracellular vesicles publication-title: J Extracell Vesicles – volume: 110 start-page: 13109 year: 2013 end-page: 13113 ident: bb0335 article-title: Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells publication-title: Proc Natl Acad Sci – volume: 200 start-page: 373 year: 2013 Feb 18 end-page: 383 ident: bb0005 article-title: Extracellular vesicles: exosomes, microvesicles, and friends publication-title: J Cell Biol – volume: 8 start-page: e72426 year: 2013 Jan ident: bb0180 article-title: A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation publication-title: PLoS One – volume: 91 start-page: 431 year: 2013 end-page: 437 ident: bb0045 article-title: Exosomes in tumor microenvironment influence cancer progression and metastasis publication-title: J Mol Med – volume: 5 start-page: 31803 year: 2016 ident: bb0125 article-title: Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids publication-title: J Extracell Vesicles – volume: 13 start-page: 269 year: 1967 end-page: 288 ident: bb0240 article-title: The nature and significance of platelet products in human plasma publication-title: Br J Haematol – volume: 10 start-page: 619 year: 2008 end-page: 624 ident: bb0015 article-title: Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells publication-title: Nat Cell Biol – volume: 368 start-page: 4439 year: 2010 end-page: 4451 ident: bb0155 article-title: High-concentration zeta potential measurements using light-scattering techniques publication-title: Philos Trans R Soc A Math Phys Eng Sci – volume: 36 start-page: 301 year: 2016 Apr 6 end-page: 312 ident: bb0230 article-title: Introduction to extracellular vesicles: biogenesis, RNA cargo selection, content, release, and uptake publication-title: Cell Mol Neurobiol – volume: 13 start-page: 258 year: 2015 ident: bb0320 article-title: eNOS polymorphisms as predictors of efficacy of bevacizumab-based chemotherapy in metastatic colorectal cancer: data from a randomized clinical trial publication-title: J Transl Med – volume: 2 year: 2013 ident: bb0300 article-title: Comparative marker analysis of extracellular vesicles in different human cancer types publication-title: J Extracell Vesicles – volume: 38 start-page: 1359 year: 2016 Nov 29 end-page: 1366 ident: bb0130 article-title: Charge-based precipitation of extracellular vesicles publication-title: Int J Mol Med – volume: 4 year: 2015 ident: bb0055 article-title: Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting publication-title: J Extracell Vesicles – volume: 113 start-page: 170 year: 2016 Jan 5 end-page: 175 ident: bb0345 article-title: Chromatographically isolated CD63+CD81+ extracellular vesicles from mesenchymal stromal cells rescue cognitive impairments after TBI publication-title: Proc Natl Acad Sci U S A – volume: 5 start-page: 31242 year: 2016 Jan 24 ident: bb0205 article-title: A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing publication-title: J Extracell Vesicles – volume: 32 start-page: 10189 year: 2016 end-page: 10198 ident: bb0145 article-title: Molecular origins of the zeta potential publication-title: Langmuir – volume: 1061 start-page: 297 year: 1991 end-page: 303 ident: bb0200 article-title: Small-volume extrusion apparatus for preparation of large, unilamellar vesicles publication-title: Biochim Biophys Acta – volume: 28 start-page: 165 year: 2017 Mar 17 end-page: 173 ident: bb0255 article-title: Flow analysis of individual blood extracellular vesicles in acute coronary syndrome publication-title: Platelets – volume: 3 start-page: 23111 year: 2014 Jan 24 ident: bb0110 article-title: The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles publication-title: J Extracell Vesicles – volume: 4 start-page: 409 year: 1993 Dec end-page: 418 ident: bb0270 article-title: The leukocyte common antigen, CD45 and other protein tyrosine phosphatases in hematopoietic cells publication-title: Semin Cell Biol – volume: 5 start-page: 32570 year: 2016 Jan 24 ident: bb0195 article-title: High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources publication-title: J Extracell Vesicles – year: 2018 ident: bb0360 article-title: Prognostic role of carcinoembryonic antigen and carbohydrate antigen 19-9 in metastatic colorectal cancer : a BRAF -mutant subset with high CA 19-9 level and poor outcome publication-title: Br J Cancer – volume: 1831 start-page: 1302 year: 2013 Jul end-page: 1309 ident: bb0190 article-title: Molecular lipidomics of exosomes released by PC-3 prostate cancer cells publication-title: Biochim Biophys Acta – volume: 394 start-page: 1253 year: 2013 Jan 1 end-page: 1262 ident: bb0105 article-title: Current methods for the isolation of extracellular vesicles publication-title: Biol Chem – volume: 98 start-page: 4 year: 2017 Feb 15 end-page: 16 ident: bb0250 article-title: Biological reference materials for extracellular vesicle studies publication-title: Eur J Pharm Sci – volume: 4 start-page: 28533 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0290 article-title: Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content publication-title: J Extracell Vesicles doi: 10.3402/jev.v4.28533 – volume: 28 start-page: 165 issue: 2 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0255 article-title: Flow analysis of individual blood extracellular vesicles in acute coronary syndrome publication-title: Platelets doi: 10.1080/09537104.2016.1212002 – volume: 30 start-page: 255 issue: 1 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0020 article-title: Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles publication-title: Annu Rev Cell Dev Biol doi: 10.1146/annurev-cellbio-101512-122326 – volume: 2 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0310 article-title: Beaming and droplet digital pcr analysis of mutant idh1 mrna in glioma patient serum and cerebrospinal fluid extracellular vesicles publication-title: Mol Ther Nucleic Acids doi: 10.1038/mtna.2013.28 – volume: 113 start-page: 170 issue: 1 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0345 article-title: Chromatographically isolated CD63+CD81+ extracellular vesicles from mesenchymal stromal cells rescue cognitive impairments after TBI publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.1522297113 – volume: 2 start-page: 698 issue: 11 year: 2001 ident: 10.1016/j.ebiom.2019.04.039_bb0350 article-title: Trends in biomarker research for cancer detection publication-title: Lancet Oncol doi: 10.1016/S1470-2045(01)00560-5 – volume: 91 start-page: 431 issue: 4 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0045 article-title: Exosomes in tumor microenvironment influence cancer progression and metastasis publication-title: J Mol Med doi: 10.1007/s00109-013-1020-6 – volume: 19 start-page: 432 issue: 3 year: 2019 ident: 10.1016/j.ebiom.2019.04.039_bb0065 article-title: Rapid and efficient isolation and detection of extracellular vesicles from plasma for lung cancer diagnosis publication-title: Lab Chip doi: 10.1039/C8LC01193A – volume: 63 start-page: 65 issue: 2 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0135 article-title: Ion-exchange chromatography purification of extracellular vesicles publication-title: Biotechniques doi: 10.2144/000114575 – volume: 90 start-page: 11122 issue: 23 year: 1993 ident: 10.1016/j.ebiom.2019.04.039_bb0315 article-title: Glyceraldehyde-3-phosphate dehydrogenase is required for the transport of nitric oxide in platelets publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.90.23.11122 – volume: 89 start-page: 663 issue: 7 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0245 article-title: Peripheral blood microvesicles secretion is influenced by storage time, temperature, and anticoagulants publication-title: Cytom Part A doi: 10.1002/cyto.a.22892 – volume: 16 start-page: 1726 issue: 12 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0365 article-title: Molecular profiling of 6,892 colorectal cancer samples suggests different possible treatment options specific to metastatic sites publication-title: Cancer Biol Ther doi: 10.1080/15384047.2015.1113356 – volume: 18 start-page: 1236 issue: 1 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0075 article-title: Liquid biopsy using the supernatant of a pleural effusion for EGFR genotyping in pulmonary adenocarcinoma patients: a comparison between cell-free DNA and extracellular vesicle-derived DNA publication-title: BMC Cancer doi: 10.1186/s12885-018-5138-3 – volume: 7 start-page: 17479 issue: 1 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0160 article-title: High-resolution single particle zeta potential characterisation of biological nanoparticles using tunable resistive pulse sensing publication-title: Sci Rep doi: 10.1038/s41598-017-14981-x – volume: 3 issue: 0 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0175 article-title: The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling publication-title: J Extracell Vesicles – volume: 12 start-page: 671 issue: 1 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0330 article-title: Protein profiling and sizing of extracellular vesicles from colorectal cancer patients via flow cytometry publication-title: ACS Nano doi: 10.1021/acsnano.7b07782 – volume: 7 start-page: 1505403 issue: 1 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0090 article-title: Large extracellular vesicles carry most of the tumour DNA circulating in prostate cancer patient plasma publication-title: J Extracell Vesicles doi: 10.1080/20013078.2018.1505403 – volume: 1831 start-page: 1302 issue: 7 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0190 article-title: Molecular lipidomics of exosomes released by PC-3 prostate cancer cells publication-title: Biochim Biophys Acta doi: 10.1016/j.bbalip.2013.04.011 – year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0305 article-title: Innovative approach of prostate cancer by the way of tumour cell-derived extracellular vesicles publication-title: J Extracell Vesicles – volume: 126 start-page: 5553 issue: 24 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0210 article-title: Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles publication-title: J Cell Sci doi: 10.1242/jcs.128868 – volume: 200 start-page: 373 issue: 4 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0005 article-title: Extracellular vesicles: exosomes, microvesicles, and friends publication-title: J Cell Biol doi: 10.1083/jcb.201211138 – volume: 6 start-page: 31175 issue: 1 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0225 article-title: Fetal bovine serum RNA interferes with the cell culture derived extracellular RNA publication-title: Sci Rep doi: 10.1038/srep31175 – volume: 4 start-page: 409 issue: 6 year: 1993 ident: 10.1016/j.ebiom.2019.04.039_bb0270 article-title: The leukocyte common antigen, CD45 and other protein tyrosine phosphatases in hematopoietic cells publication-title: Semin Cell Biol doi: 10.1006/scel.1993.1049 – volume: 6 start-page: 33935 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0340 article-title: A novel affinity-based method for the isolation of highly purified extracellular vesicles publication-title: Sci Rep doi: 10.1038/srep33935 – volume: 368 start-page: 4439 issue: 1927 year: 2010 ident: 10.1016/j.ebiom.2019.04.039_bb0155 article-title: High-concentration zeta potential measurements using light-scattering techniques publication-title: Philos Trans R Soc A Math Phys Eng Sci doi: 10.1098/rsta.2010.0175 – volume: 164 start-page: 1226 issue: 6 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0035 article-title: Communication by extracellular vesicles: where we are and where we need to go publication-title: Cell doi: 10.1016/j.cell.2016.01.043 – volume: 14 start-page: 228 issue: 3 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0095 article-title: EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research publication-title: Nat Methods doi: 10.1038/nmeth.4185 – volume: 394 start-page: 1253 issue: 10 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0105 article-title: Current methods for the isolation of extracellular vesicles publication-title: Biol Chem doi: 10.1515/hsz-2013-0141 – volume: 4 start-page: 356 issue: 3 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0215 article-title: Endocytic trafficking of membrane-bound cargo: a flotillin point of view publication-title: Membranes (Basel) doi: 10.3390/membranes4030356 – year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0120 article-title: Rapid magnetic isolation of extracellular vesicles via lipid-based nanoprobes publication-title: Nat Biomed Eng doi: 10.1038/s41551-017-0058 – volume: 38 start-page: 1359 issue: 5 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0130 article-title: Charge-based precipitation of extracellular vesicles publication-title: Int J Mol Med doi: 10.3892/ijmm.2016.2759 – year: 2006 ident: 10.1016/j.ebiom.2019.04.039_bb0030 article-title: Isolation and characterization of exosomes from cell culture supernatants and biological fluids publication-title: Curr Protoc Cell Biol doi: 10.1002/0471143030.cb0322s30 – volume: 1061 start-page: 297 issue: 2 year: 1991 ident: 10.1016/j.ebiom.2019.04.039_bb0200 article-title: Small-volume extrusion apparatus for preparation of large, unilamellar vesicles publication-title: Biochim Biophys Acta doi: 10.1016/0005-2736(91)90295-J – volume: 13 start-page: 269 issue: 3 year: 1967 ident: 10.1016/j.ebiom.2019.04.039_bb0240 article-title: The nature and significance of platelet products in human plasma publication-title: Br J Haematol doi: 10.1111/j.1365-2141.1967.tb08741.x – volume: 181 start-page: 1573 issue: 5 year: 2012 ident: 10.1016/j.ebiom.2019.04.039_bb0170 article-title: Large oncosomes in human prostate cancer tissues and in the circulation of mice with metastatic disease publication-title: Am J Pathol doi: 10.1016/j.ajpath.2012.07.030 – volume: 8 start-page: 5730 issue: 1 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0140 article-title: Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography publication-title: Sci Rep doi: 10.1038/s41598-018-24163-y – volume: 105 start-page: 392 issue: 4 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0235 article-title: Extracellular vesicles and blood diseases publication-title: Int J Hematol doi: 10.1007/s12185-017-2180-x – volume: 2 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0300 article-title: Comparative marker analysis of extracellular vesicles in different human cancer types publication-title: J Extracell Vesicles doi: 10.3402/jev.v2i0.20424 – volume: 10 start-page: 619 issue: 5 year: 2008 ident: 10.1016/j.ebiom.2019.04.039_bb0015 article-title: Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells publication-title: Nat Cell Biol doi: 10.1038/ncb1725 – volume: 5 start-page: 32570 issue: 1 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0195 article-title: High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources publication-title: J Extracell Vesicles doi: 10.3402/jev.v5.32570 – volume: 5 start-page: 31803 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0125 article-title: Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids publication-title: J Extracell Vesicles doi: 10.3402/jev.v5.31803 – volume: 18 start-page: 158 issue: 3 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0080 article-title: Detection of mutant KRAS and TP53 DNA in circulating exosomes from healthy individuals and patients with pancreatic cancer publication-title: Cancer Biol Ther doi: 10.1080/15384047.2017.1281499 – volume: 110 start-page: 13109 issue: 32 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0335 article-title: Exosome-mediated transmission of hepatitis C virus between human hepatoma Huh7.5 cells publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.1221899110 – volume: 4 start-page: 29260 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0220 article-title: Handling and storage of human body fluids for analysis of extracellular vesicles publication-title: J Extracell Vesicles doi: 10.3402/jev.v4.29260 – volume: 148 issue: 1 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0355 article-title: Association between molecular subtypes of colorectal cancer and patient survival publication-title: Gastroenterology doi: 10.1053/j.gastro.2014.09.038 – volume: 7 start-page: 1293 issue: 1 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0325 article-title: Circulating VEGF and eNOS variations as predictors of outcome in metastatic colorectal cancer patients receiving bevacizumab publication-title: Sci Rep doi: 10.1038/s41598-017-01420-0 – volume: 60 start-page: 15 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0040 article-title: The influence of tumour-derived extracellular vesicles on local and distal metastatic dissemination publication-title: Mol Aspects Med doi: 10.1016/j.mam.2017.11.012 – volume: 13 start-page: 258 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0320 article-title: eNOS polymorphisms as predictors of efficacy of bevacizumab-based chemotherapy in metastatic colorectal cancer: data from a randomized clinical trial publication-title: J Transl Med doi: 10.1186/s12967-015-0619-5 – volume: 98 start-page: 4 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0250 article-title: Biological reference materials for extracellular vesicle studies publication-title: Eur J Pharm Sci doi: 10.1016/j.ejps.2016.09.008 – year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0115 article-title: Nanoplasmonic quantification of tumour-derived extracellular vesicles in plasma microsamples for diagnosis and treatment monitoring publication-title: Nat Biomed Eng doi: 10.1038/s41551-016-0021 – volume: 194 start-page: 243 year: 2019 ident: 10.1016/j.ebiom.2019.04.039_bb0070 article-title: EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform publication-title: Talanta doi: 10.1016/j.talanta.2018.10.016 – volume: 28 start-page: 741 issue: 4 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0085 article-title: High prevalence of mutant KRAS in circulating exosome-derived DNA from early-stage pancreatic cancer patients publication-title: Ann Oncol doi: 10.1093/annonc/mdx004 – volume: 8 start-page: e72426 issue: 8 year: 2013 ident: 10.1016/j.ebiom.2019.04.039_bb0180 article-title: A novel high throughput biochemical assay to evaluate the HuR protein-RNA complex formation publication-title: PLoS One doi: 10.1371/journal.pone.0072426 – volume: 3 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0265 article-title: Isolation and characterization of platelet-derived extracellular vesicles publication-title: J Extracell Vesicles doi: 10.3402/jev.v3.24692 – volume: 5 start-page: 31242 issue: 1 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0205 article-title: A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing publication-title: J Extracell Vesicles doi: 10.3402/jev.v5.31242 – volume: 4 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0055 article-title: Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting publication-title: J Extracell Vesicles doi: 10.3402/jev.v4.26316 – volume: 49 start-page: 6562 issue: 23 year: 1989 ident: 10.1016/j.ebiom.2019.04.039_bb0275 article-title: Depletion of T-cells with the CD4+CD45R+ phenotype in lymphocytes that infiltrate subcutaneous metastases of human melanoma publication-title: Cancer Res – volume: 13 start-page: 1248 issue: 9 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0370 article-title: IASLC statement paper: liquid biopsy for advanced non-small cell lung cancer (NSCLC) publication-title: J Thorac Oncol doi: 10.1016/j.jtho.2018.05.030 – volume: 3 start-page: 25011 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0295 article-title: Comparative analysis of discrete exosome fractions obtained by differential centrifugation publication-title: J Extracell Vesicles doi: 10.3402/jev.v3.25011 – volume: 1861 start-page: 3164 issue: 1 Pt A year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0165 article-title: Methods for the physical characterization and quantification of extracellular vesicles in biological samples publication-title: Biochim Biophys Acta doi: 10.1016/j.bbagen.2016.07.028 – volume: 1165 start-page: 147 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0010 article-title: Microvesicles as mediators of intercellular communication in cancer publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-0856-1_11 – volume: 36 start-page: 301 issue: 3 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0230 article-title: Introduction to extracellular vesicles: biogenesis, RNA cargo selection, content, release, and uptake publication-title: Cell Mol Neurobiol doi: 10.1007/s10571-016-0366-z – volume: 7 start-page: 12404 issue: 12 year: 2012 ident: 10.1016/j.ebiom.2019.04.039_bb0150 article-title: Zeta potentials and isoelectric points of biomolecules: the effects of ion types and ionic strengths publication-title: Int J Electrochem Sci doi: 10.1016/S1452-3981(23)16554-0 – volume: 10 start-page: 3886 issue: 4 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0060 article-title: Evidence-based clinical use of nanoscale extracellular vesicles in nanomedicine publication-title: ACS Nano doi: 10.1021/acsnano.5b08015 – volume: 290 start-page: 24902 issue: 41 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0185 article-title: Structural basis for the discriminative recognition of N 6 -methyladenosine RNA by the human YT521-B homology domain family of proteins publication-title: J Biol Chem doi: 10.1074/jbc.M115.680389 – volume: 28 start-page: 214 issue: 3 year: 2017 ident: 10.1016/j.ebiom.2019.04.039_bb0260 article-title: Platelet microvesicles in health and disease publication-title: Platelets doi: 10.1080/09537104.2016.1265924 – year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0360 article-title: Prognostic role of carcinoembryonic antigen and carbohydrate antigen 19-9 in metastatic colorectal cancer : a BRAF -mutant subset with high CA 19-9 level and poor outcome publication-title: Br J Cancer doi: 10.1038/s41416-018-0115-9 – volume: 4 start-page: 27066 year: 2015 ident: 10.1016/j.ebiom.2019.04.039_bb0050 article-title: Biological properties of extracellular vesicles and their physiological functions publication-title: J Extracell Vesicles doi: 10.3402/jev.v4.27066 – volume: 32 start-page: 10189 issue: 40 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0145 article-title: Molecular origins of the zeta potential publication-title: Langmuir doi: 10.1021/acs.langmuir.6b02493 – volume: 5 issue: 0 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0100 article-title: Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey publication-title: J Extracell Vesicles – volume: 3 start-page: 23111 issue: 1 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0110 article-title: The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles publication-title: J Extracell Vesicles doi: 10.3402/jev.v3.23111 – volume: 19 start-page: 213 issue: 4 year: 2018 ident: 10.1016/j.ebiom.2019.04.039_bb0025 article-title: Shedding light on the cell biology of extracellular vesicles publication-title: Nat Rev Mol Cell Biol doi: 10.1038/nrm.2017.125 – volume: 5 start-page: 25355 year: 2016 ident: 10.1016/j.ebiom.2019.04.039_bb0280 article-title: Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples publication-title: J Extracell Vesicles doi: 10.3402/jev.v5.25355 – volume: 5 start-page: 3591 year: 2014 ident: 10.1016/j.ebiom.2019.04.039_bb0285 article-title: Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen publication-title: Nat Commun doi: 10.1038/ncomms4591 – reference: 31101598 - EBioMedicine. 2019 Jun;44:14-15. doi: 10.1016/j.ebiom.2019.05.021 |
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| Snippet | Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and... AbstractBackgroundExtracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient... |
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| SubjectTerms | Advanced Basic Science Alpha Biomarkers Biomarkers, Tumor - blood Cancer Case-Control Studies Cell Line, Tumor Droplet PCR Extracellular vesicles Extracellular Vesicles - metabolism Extracellular Vesicles - ultrastructure Flow Cytometry Humans Internal Medicine Liquid biopsy Liquid Biopsy - methods Liquid Biopsy - standards Neoplasms - blood Neoplasms - diagnosis Neoplasms - genetics Neoplasms - metabolism Nickel Polymerase Chain Reaction Research paper Sensitivity and Specificity Ultracentrifugation |
| Title | Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood |
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