Regulation of membrane type-1 matrix metalloproteinase activity and intracellular localization in clinical thoracic aortic aneurysms

• Published in: The Journal of thoracic and cardiovascular surgery Vol. 153; no. 3; pp. 537 - 546
• Main Authors: Ikonomidis, John S., Nadeau, Elizabeth K., Akerman, Adam W., Stroud, Robert E., Mukherjee, Rupak, Jones, Jeffrey A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2017
• Subjects: Aged; aneurysm; Animals; Aorta, Thoracic - enzymology; Aorta, Thoracic - pathology; Aortic Aneurysm, Thoracic - enzymology; Aortic Aneurysm, Thoracic - pathology; Cardiothoracic Surgery; Cell Line; Disease Models, Animal; Enzyme Activation - physiology; Fibroblasts - metabolism; Fibroblasts - pathology; Humans; Immunoblotting; Intracellular Space - enzymology; Matrix Metalloproteinase 14 - metabolism; Mice; Microscopy, Confocal; Middle Aged; MT1-MMP; protein kinase C; remodeling; thoracic aorta; Vascular Remodeling; PBS; aneurysm; remodeling; PMA; thoracic aorta; PKC; ECM; GFP; MMP; MT1-MMP; TGF; TAA; protein kinase C; phorbol 12-myristate 13-acetate; phosphate-buffered saline; green fluorescent protein; thoracic aortic aneurysm; membrane type-1 matrix metalloproteinase; matrix metalloproteinase; transforming growth factor; extracellular matrix

Membrane type-1 matrix metalloproteinase (MT1-MMP) is elevated during thoracic aortic aneurysm (TAA) development in mouse models, and plays an important role in the activation of matrix metalloproteinase (MMP)-2 and the release of matrix- bound transforming growth factor-β. In this study, we tested...