Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time

• Published in: Journal of assisted reproduction and genetics Vol. 29; no. 2; pp. 153 - 158
• Main Authors: Mohammadi-Sangcheshmeh, Abdollah, Soleimani, Masoud, Deldar, Hamid, Salehi, Mohammad, Soudi, Sara, Hashemi, Seyed Mahmoud, Schellander, Karl, Hoelker, Michael
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.02.2012; Springer Nature B.V
• Subjects: Animal sciences; Animals; Blastocyst - cytology; blastocysts; Cytoplasm; Dehydrogenases; Embryo Transfer - methods; Embryos; Enzymes; Female; Fertilization; Fertilization in Vitro - methods; Gamete Biology; Glucose; Glucosephosphate dehydrogenase; Glucosephosphate Dehydrogenase - metabolism; Gynecology; Human Genetics; In Vitro Oocyte Maturation Techniques; Medicine; Medicine & Public Health; Morphology; Oocytes; Oocytes - enzymology; Oocytes - growth & development; Ovaries; Predictive Value of Tests; Prognosis; Reproductive Medicine; Reproductive technologies; Sheep - embryology; Zoology; Iran; United States > US; BCB; Ovine; Glucose-6-phosphate dehydrogenase; Developmental competence; Oocyte
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1007/s10815-011-9625-6

Purpose To determine whether G6PDH-activity measured by Brilliant Cresyl Blue known as BCB dye, predicts developmental competence within cohorts of ovine oocytes. Methods Ovine oocytes were exposed to BCB staining and categorized into two groups: BCB+ (blue cytoplasm, low G6PDH-activity) and BCB- (colorless cytoplasm, high G6PDH-activity). After maturation in vitro, oocytes were subjected to fertilization followed by in vitro embryo culture. Results We observed a significant difference in oocyte diameter considering BCB+ and BCB- oocytes. BCB+ and Control groups showed significantly higher maturation rates compared to BCB- group. There were significantly more cleaved embryos in BCB+ and control groups than in BCB- group. Blastocyst rate was significantly higher for BCB+ group compared to control and BCB- groups with control group being significantly higher than BCB- group. Conclusion G6PDH-activity is a strong predictive marker of oocyte competence and may be useful in identifying oocytes with a good prognosis for further develop.