Purification and molecular cloning of xylanases from the wood-feeding termite, Coptotermes formosanus Shiraki

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 3; pp. 710 - 718
• Main Authors: Arakawa, G.(Ryukyu Univ., Nishihara, Okinawa (Japan)), Watanabe, H, Yamasaki, H, Maekawa, H, Tokuda, G
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 23.03.2009; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Amino Acid Sequence; Animals; APPAREIL DIGESTIF; Base Sequence; Biological and medical sciences; Chromatography; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; Cloning, Molecular; Coptotermes formosanus; DEGRADACION; DEGRADATION; DIGESTIVE SYSTEM; DNA, Complementary - genetics; ENZIMAS; ENZYME; ENZYMES; Fundamental and applied biological sciences. Psychology; Gastrointestinal Tract - enzymology; GHF11; ISOPTERA; Isoptera - enzymology; Isoptera - genetics; Isoptera - physiology; MASTIGOPHORA; MOLECULAR CLONING; Molecular Sequence Data; Nucleic Acid Amplification Techniques; PCR; protist; PURIFICACION; PURIFICATION; SIMBIONTICO; SISTEMA DIGESTIVO; SYMBIONTS; Symbiosis; SYMBIOTE; termite; XILANOS; xylan; xylanase; XYLANE; XYLANS; Xylosidases - chemistry; Xylosidases - genetics; Xylosidases - isolation & purification; Xylosidases - metabolism; Purification; Wood; Enzyme; Insecta; Coptotermes formosanus; Isoptera; Xylan; Endo-1,4-β-xylanase; GHF11; xylanase; Glycosylases; Termite; Arthropoda; Glycosidases; protist; Hydrolases; Rhinotermitidae; Xylan endo-1,3-β-xylosidase; Invertebrata; Molecular cloning

Coptotermes formosanus is one of the most destructive termites in the southern part of Japan as well as in the United States. Hemicellulose is a noncellulosic polysaccharide found in plant cell walls, and xylan is the major constituent of hemicellulose. Since hemicellulose prevents access of cellulo...