Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles - Wang, Ming; Zuris, John A.; Meng, Fantao; Rees, Holly; Sun, Shuo; Deng, Pu; Han, Yong; Gao, Xue; Pouli, Dimitra; Wu, Qi; Georgakoudi, Irene; Liu, David R.; et al. (Proceedings of the National Academy of Sciences - PNAS, 2016)
Keywords: Animals; Bacterial Proteins - administration & dosage; Bacterial Proteins - genetics; Ceramides - chemistry; Cholesterol - chemistry; CRISPR-Cas Systems; Drug Carriers; Endocytosis; Endonucleases - administration & dosage; Endonucleases - genetics; Endosomes - metabolism; Gene Knockout Techniques; Genes, Reporter; Genes, Synthetic; Genetic Engineering - methods; Genomes; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; HeLa Cells; Humans; Hypothalamus - metabolism; Integrases - administration & dosage; Integrases - genetics; Lipids; Lipids - administration & dosage; Lipids - chemical synthesis; Lipids - chemistry; Luminescent Proteins - biosynthesis; Luminescent Proteins - genetics; Mice; Molecular Structure; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Nanoparticles - metabolism; Nanoparticles - toxicity; Phosphatidylethanolamines - chemistry; Physical Sciences; Proteins; Recombinant Proteins - biosynthesis; Recombination, Genetic; Ribonucleic acid; RNA; RNA - genetics; Static Electricity; Structure-Activity Relationship; Thalamus - metabolism; genome editing; lipid nanoparticle; CRISPR/Cas9; protein delivery; Cre recombinase