Generation of Induced Pluripotent Stem Cells from Human Amniotic Fluid Cells by Reprogramming with Two Factors in Feeder-free Conditions

• Published in: Journal of Reproduction and Development Vol. 59; no. 1; pp. 72 - 77
• Main Authors: LI, Qing, FAN, Yong, SUN, Xiaofang, YU, Yanhong
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 2013; The Society for Reproduction and Development
• Subjects: Amniotic Fluid - cytology; Cell Culture Techniques; Cell Differentiation; Cells, Cultured; Cellular Reprogramming; Feeder Cells - cytology; Feeder-free; Gene Expression Regulation, Developmental; HEK293 Cells; Human amniotic fluid cells; Humans; Induced pluripotent stem cells; Induced Pluripotent Stem Cells - cytology; Karyotyping; Kruppel-Like Transcription Factors - metabolism; Mutagenesis; Neural Stem Cells - cytology; Octamer Transcription Factor-3 - metabolism; Oligonucleotide Array Sequence Analysis; Original; Transcription Factors - metabolism

The ectopic expression of transcription factors for reprogramming human somatic cells to a pluripotent state represents a valuable resource for the development of in vitro-based models for human disease and has great potential in regenerative therapies. However, the majority of studies have used ski...