The Assembly-Activating Protein Promotes Stability and Interactions between AAV’s Viral Proteins to Nucleate Capsid Assembly

• Published in: Cell reports (Cambridge) Vol. 23; no. 6; pp. 1817 - 1830
• Main Authors: Maurer, Anna C., Pacouret, Simon, Cepeda Diaz, Ana Karla, Blake, Jessica, Andres-Mateos, Eva, Vandenberghe, Luk H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.05.2018; Elsevier
• Subjects: AAP; AAV; adeno-associated virus; Amino Acid Motifs; capsid; capsid assembly; Capsid Proteins - chemistry; Capsid Proteins - metabolism; Dependovirus - pathogenicity; Dependovirus - physiology; Dependovirus - ultrastructure; Gain of Function Mutation; gene therapy; HEK293 Cells; Human health and pathology; Humans; Life Sciences; manufacturing; Models, Molecular; Phenotype; Phylogeny; Protein Binding; Protein Multimerization; Protein Stability; Serotyping; structure-function; vector engineering; Virion - pathogenicity; Virion - ultrastructure; Virus Assembly; AAP; AAV; capsid assembly; capsid; manufacturing; structure-function; gene therapy; adeno-associated virus; vector engineering; vector; engineering

The adeno-associated virus (AAV) vector is a preferred delivery platform for in vivo gene therapy. Natural and engineered variations of the AAV capsid affect a plurality of phenotypes relevant to gene therapy, including vector production and host tropism. Fundamental to these aspects is the mechanis...