Glycotripod Amphiphiles for Solubilization and Stabilization of a Membrane-Protein Superassembly: Importance of Branching in the Hydrophilic Portion

Three‐legged friends: Intrinsic membrane proteins must usually be extracted from the native membrane with the aid of synthetic amphiphiles and then stabilized before detailed structural and functional characterization is possible. We describe new amphiphiles with unusual architectures that were usef...

Full description

Saved in:
Bibliographic Details
Published inChembiochem : a European journal of chemical biology Vol. 9; no. 11; pp. 1706 - 1709
Main Authors Chae, Pil S, Wander, Marc J, Bowling, Aaron P, Laible, Philip D, Gellman, Samuel H
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 21.07.2008
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects
amphiphiles
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Carbohydrates - chemical synthesis
Carbohydrates - chemistry
Carbohydrates - pharmacology
Cell Membrane - chemistry
Cell Membrane - metabolism
detergent design
membrane proteins
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Protein Binding - drug effects
Rhodobacter capsulatus - chemistry
Solubility - drug effects
solubilization
stabilization
Online AccessGet full text

Cover

Abstract Three‐legged friends: Intrinsic membrane proteins must usually be extracted from the native membrane with the aid of synthetic amphiphiles and then stabilized before detailed structural and functional characterization is possible. We describe new amphiphiles with unusual architectures that were useful for extraction and stabilization of protein superassemblies from bacterial membranes. Our results suggest that incorporation of branch points (as shown in figure) in both the hydrophilic and lipophilic portions can lead to favorable amphiphile behavior.
AbstractList Intrinsic membrane proteins must usually be extracted from the native membrane with the aid of synthetic amphiphiles and then stabilized in a soluble form before detailed structural and functional characterization is possible. We describe new amphiphiles with unusual architectures that are useful for extraction and stabilization of photosynthetic protein superassemblies from bacterial membranes. Our results suggest that incorporating branch points in both the hydrophilic and lipophilic portions can lead to favorable amphiphile behavior.
Three‐legged friends: Intrinsic membrane proteins must usually be extracted from the native membrane with the aid of synthetic amphiphiles and then stabilized before detailed structural and functional characterization is possible. We describe new amphiphiles with unusual architectures that were useful for extraction and stabilization of protein superassemblies from bacterial membranes. Our results suggest that incorporation of branch points (as shown in figure) in both the hydrophilic and lipophilic portions can lead to favorable amphiphile behavior.
Author Laible, Philip D.
Wander, Marc J.
Bowling, Aaron P.
Gellman, Samuel H.
Chae, Pil S.
AuthorAffiliation [b] Biosciences Division, Argonne National Laborotory 9700 South Cass Avenue, Argonne, IL 60439
[a] Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706 (USA)
AuthorAffiliation_xml – name: [b] Biosciences Division, Argonne National Laborotory 9700 South Cass Avenue, Argonne, IL 60439
– name: [a] Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706 (USA)
Author_xml – sequence: 1
  fullname: Chae, Pil S
– sequence: 2
  fullname: Wander, Marc J
– sequence: 3
  fullname: Bowling, Aaron P
– sequence: 4
  fullname: Laible, Philip D
– sequence: 5
  fullname: Gellman, Samuel H
BackLink https://www.ncbi.nlm.nih.gov/pubmed/18576450$$D View this record in MEDLINE/PubMed
BookMark eNqFkkuP0zAUhSM0iHnAliV4xS7Fj8SOWSDNVEynYhiKygiJjWU7TmtI4mAnQPgd_GBcpSplhWTJ1vV3zr3y8Xly0rrWJMlTBGcIQvxSK6tnGMICQkT5g-QMZYSnjBJysj9nGLPT5DyELxBCTgl6lJyiImc0y-FZ8ntRj9r13nauBJdNt7Vx1SaAynmwdvWgbG1_yd66Fsi2BOteHlVcBSR4ZxrlZWvSlXe9sS1YD53xMoRYr8dXYNl0zvey1WbHX0VUb227AZHstwbcjKV3u6ZWg1UEo-_j5GEl62Ce7PeL5P76zcf5TXr7frGcX96mOqeUp5wxLQ3FJaQq1zzjBGFpsCIFR0WpiMGmUIqgkiGkK1ZhmGOFec41MShTFblIXk--3aAaU2rT9l7WovO2kX4UTlrx701rt2LjvguSY8YhjgYv9gbefRtM6EVjgzZ1HZ_DDUHQOBKlLIvgbAK1dyF4Ux2aICh2QYpdkOIQZBQ8Ox7tL75PLgJ8An7EuMb_2In51XJ-bJ5OWht68_Oglf6roIywXHy6WwhCrj_A1ds78Tnyzye-kk7IjbdB3K8xRCR-KEQLkpM_LyPJEg
CitedBy_id crossref_primary_10_1021_la904893d
crossref_primary_10_1039_C5AN00240K
crossref_primary_10_1021_jacs_5b13233
crossref_primary_10_1038_aps_2011_187
crossref_primary_10_1039_C2CC36844G
crossref_primary_10_1039_c3mb25584k
crossref_primary_10_1002_bkcs_10416
crossref_primary_10_1039_C6CC06147H
crossref_primary_10_1038_nmeth_1526
crossref_primary_10_1002_asia_201301097
crossref_primary_10_1016_j_bioorg_2015_07_003
crossref_primary_10_1039_c4nj00033a
crossref_primary_10_1002_asia_202200941
crossref_primary_10_1039_C7AN01168G
crossref_primary_10_1016_j_ymeth_2018_02_017
crossref_primary_10_1002_asia_201301303
crossref_primary_10_1002_chem_201301423
crossref_primary_10_1021_acs_bioconjchem_3c00042
crossref_primary_10_1039_C4OB01375A
crossref_primary_10_1002_chem_201600533
crossref_primary_10_1016_j_colsurfa_2015_07_054
crossref_primary_10_1021_ja9085148
crossref_primary_10_1039_C8OB03153C
crossref_primary_10_1039_C7SC03700G
crossref_primary_10_1021_jacs_6b11997
crossref_primary_10_1021_acschembio_0c00316
crossref_primary_10_1002_cbic_201500151
crossref_primary_10_1002_chem_201200069
crossref_primary_10_1039_C4AY03091E
crossref_primary_10_1016_j_bbagen_2018_11_004
crossref_primary_10_1039_C4CC05746E
crossref_primary_10_1146_annurev_biochem_052208_114057
crossref_primary_10_1002_asia_201900224
crossref_primary_10_1002_jsde_12216
crossref_primary_10_1016_j_bbamem_2013_09_011
crossref_primary_10_1039_C8OB00270C
crossref_primary_10_1002_cbic_201200759
crossref_primary_10_1002_chem_201501083
crossref_primary_10_1002_chem_201603338
crossref_primary_10_1002_cbic_201600429
crossref_primary_10_1038_s41598_017_03809_3
crossref_primary_10_1039_C5SC02900G
crossref_primary_10_1039_B915162C
crossref_primary_10_1007_s00726_013_1600_3
crossref_primary_10_1021_acs_biochem_0c00183
crossref_primary_10_1021_acs_cgd_2c00901
Cites_doi 10.1074/jbc.R100031200
10.1016/S0959-440X(97)80080-2
10.1093/emboj/18.3.534
10.1002/(SICI)1521-3773(20000218)39:4<758::AID-ANIE758>3.0.CO;2-V
10.1016/S0959-440X(00)00223-2
10.1007/s00018-006-6252-y
10.1126/science.1088892
10.1016/S1047-8477(03)00045-5
10.1038/nbt776
10.1016/j.ymeth.2007.01.007
10.1110/ps.04712004
10.1126/science.8235592
10.1021/bi026959b
10.1016/j.bbamem.2004.04.011
10.1016/S1047-8477(03)00043-1
10.1016/j.pbiomolbio.2004.07.006
10.1038/sj.emboj.7600092
10.1073/pnas.77.11.6368
10.1110/ps.9.12.2518
10.1016/S1359-0294(01)00107-8
10.1021/j100881a041
10.1002/ange.200604276
10.1023/B:PRES.0000036883.56959.a9
10.1074/jbc.M411334200
10.1002/anie.199211241
10.1002/ange.19921040905
10.1017/S0033583501003754
10.1073/pnas.0607167103
10.1007/BF00018263
10.1016/j.bbapap.2005.04.011
10.1002/(SICI)1521-3757(20000218)112:4<774::AID-ANGE774>3.0.CO;2-W
10.1002/anie.200604276
ContentType Journal Article
Copyright Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright_xml – notice: Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
DBID FBQ
BSCLL
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
DOI 10.1002/cbic.200800169
DatabaseName AGRIS
Istex
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList

Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: FBQ
  name: AGRIS
  url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN
  sourceTypes: Publisher
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
Chemistry
EISSN 1439-7633
EndPage 1709
ExternalDocumentID 10_1002_cbic_200800169
18576450
CBIC200800169
ark_67375_WNG_33FQ0PKN_Z
US201300916835
Genre shortCommunication
Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NIH
  funderid: P01 GM75913
– fundername: NIGMS NIH HHS
  grantid: P01 GM75913
– fundername: NIGMS NIH HHS
  grantid: P01 GM075913
GroupedDBID ---
-DZ
-~X
.GJ
05W
0R~
1L6
1OC
29B
31~
33P
3WU
4.4
4ZD
50Y
53G
5GY
5VS
66C
6J9
6P2
77Q
8-0
8-1
8UM
A00
AAESR
AAHHS
AAIHA
AAJUZ
AANLZ
AASGY
AAXRX
AAZKR
ABCUV
ABCVL
ABHUG
ABIJN
ABJNI
ABLJU
ACAHQ
ACBWZ
ACCFJ
ACCZN
ACGFS
ACIWK
ACPOU
ACPRK
ACXBN
ACXQS
ADBBV
ADDAD
ADKYN
ADMGS
ADOZA
ADXAS
ADZMN
ADZOD
AEEZP
AEGXH
AEIGN
AENEX
AEQDE
AEUQT
AEUYR
AFBPY
AFFNX
AFFPM
AFGKR
AFRAH
AFZJQ
AGJLS
AHMBA
AI.
AIURR
AIWBW
AJBDE
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMYDB
ASPBG
AVWKF
AZFZN
AZVAB
BDRZF
BFHJK
BMXJE
BRXPI
CS3
DCZOG
DPXWK
DR2
DRFUL
DRSTM
DU5
EBD
EBS
EJD
EMOBN
F5P
FBQ
FEDTE
G-S
GNP
GODZA
HBH
HHY
HHZ
HVGLF
HZ~
IH2
IX1
JPC
KQQ
LATKE
LAW
LEEKS
LITHE
LOXES
LUTES
LYRES
MEWTI
MXFUL
MXSTM
MY~
NNB
O9-
P2P
P2W
P4E
PQQKQ
R.K
ROL
RWI
RX1
SUPJJ
SV3
V2E
VH1
W99
WBKPD
WH7
WJL
WOHZO
WXSBR
WYJ
XFK
XPP
XSW
XV2
Y6R
YZZ
ZGI
ZZTAW
~KM
~S-
AHBTC
AITYG
BSCLL
HGLYW
OIG
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
5PM
ID FETCH-LOGICAL-c5669-977cae62d06b5c949312ae2b38918db3e2e8bb31d711cf7f2052b2959c3e14bf3
IEDL.DBID DR2
ISSN 1439-4227
IngestDate Tue Sep 17 21:25:29 EDT 2024
Fri Aug 16 09:08:57 EDT 2024
Fri Aug 23 03:05:50 EDT 2024
Sat Sep 28 07:46:47 EDT 2024
Sat Aug 24 00:56:04 EDT 2024
Wed Oct 30 09:53:09 EDT 2024
Wed Dec 27 19:10:51 EST 2023
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 11
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c5669-977cae62d06b5c949312ae2b38918db3e2e8bb31d711cf7f2052b2959c3e14bf3
Notes http://dx.doi.org/10.1002/cbic.200800169
istex:DDAC594FF9C73496AD196A5C208FA21DD3B2D3C8
ArticleID:CBIC200800169
NIH - No. P01 GM75913
ark:/67375/WNG-33FQ0PKN-Z
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://europepmc.org/articles/pmc3527902?pdf=render
PMID 18576450
PQID 69316674
PQPubID 23479
PageCount 4
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_3527902
proquest_miscellaneous_69316674
crossref_primary_10_1002_cbic_200800169
pubmed_primary_18576450
wiley_primary_10_1002_cbic_200800169_CBIC200800169
istex_primary_ark_67375_WNG_33FQ0PKN_Z
fao_agris_US201300916835
PublicationCentury 2000
PublicationDate July 21, 2008
PublicationDateYYYYMMDD 2008-07-21
PublicationDate_xml – month: 07
  year: 2008
  text: July 21, 2008
  day: 21
PublicationDecade 2000
PublicationPlace Weinheim
PublicationPlace_xml – name: Weinheim
– name: Germany
PublicationTitle Chembiochem : a European journal of chemical biology
PublicationTitleAlternate ChemBioChem
PublicationYear 2008
Publisher Wiley-VCH Verlag
WILEY-VCH Verlag
WILEY‐VCH Verlag
Publisher_xml – name: Wiley-VCH Verlag
– name: WILEY-VCH Verlag
– name: WILEY‐VCH Verlag
References For leading references, see: M. Caffrey, J. Struct. Biol. 2003, 142, 108-132.
C.-L. McGregor, L. Chen, N. C. Pomroy, P. Hwang, S. Go, A. Chakrabartty, G. G. Privé, Nat. Biotechnol. 2003, 21, 171-176
P. J. Loll, J. Struct. Biol. 2003, 142, 144-153
Angew. Chem. Int. Ed. Engl. 1992, 31, 1124-1134.
S. Wang, S. Lin, N. W. Woodbury, J. P. Allen, Photosynth. Res. 1994, 42, 203-215.
Q. Zhang, X. Ma, A. Ward, W.-X. Hong, V.-P. Jaakola, R. C. Stevens, M. G. Finn, G. Chang, Angew. Chem. 2007, 119, 1756-1759
H. Schott, J. Phys. Chem. 1966, 70, 2966-2973.
M. J. Theisen, T. B. Potocky, D. T. McQuade, S. H. Gellman, M. L. Chiu, Biochim. Biophys. Acta Proteins Proteomics 2005, 1751, 213-216.
S. H. White, Protein Sci. 2004, 13, 1948-1949
P. Nollert, Prog. Biophys. Mol. Biol. 2005, 88, 339-357
L. M. Hjelmeland, Proc. Natl. Acad. Sci. USA 1980, 77, 6368-6370.
S. Scheuring, J. Busselez, D. Lévy, J. Biol. Chem. 2005, 280, 1426-1431.
S. M. Yu, D. T. McQuade, M. A. Quinn, C. P. Hackenberger, M. P. Krebs, A. S. Polans, S. H. Gellman, Protein Sci. 2000, 9, 2518-2527
R. M. Garavito, S. Ferguson-Miller, J. Biol. Chem. 2001, 276, 32403-32406
R. J. Cogdell, A. T. Gardiner, A. W. Roszak, C. J. Law, J. Southall, N. W. Isaacs, Photosynth. Res. 2004, 81, 207-214
D. T. McQuade, M. A. Quinn, S. M. Yu, A. S. Polans, M. P. Krebs, S. H. Gellman, Angew. Chem. 2000, 112, 774-777
G. G. Privé, Methods 2007, 41, 388-397.
Angew. Chem. Int. Ed. 2000, 39, 758-761
Angew. Chem. Int. Ed. 2007, 46, 1726-1729
For leading references, see: R. W. Hoffmann, Angew. Chem. 1992, 104, 1147-1157
J. U. Bowie, Curr. Opin. Struct. Biol. 2001, 11, 397-402
K. Lundstrom, Cell. Mol. Life Sci. 2006, 63, 2597-2607
X. Hu, T. Ritz, A. Damjanovic, F. Autenrieth, K. Schulten, Quart. Rev. Biophys. 2002, 35, 1-62
C. E. Schafmeister, L. J. W. Miercke, R. M. Stroud, Science 1993, 262, 734-738
C. Jungas, J. L. Ranck, J. L. Rigaud, P. Joliot, A. Verméglio, EMBO J. 1999, 18, 534-542
A. W. Roszak, T. D. Howard, J. Southall, A. T. Gardiner, C. J. Law, N. W. Isaacs R. J. Cogdell, Science 2003, 302, 1969-1972; the structural arrangement is also inferred from structural studies on the LHI-RC superassembly from other purple nonsulfur bacteria (cryoelectron microscopy and atomic force microscopy)
C. A. Siebert, P. Qian, D. Fotiadis, A. Engel, C. N. Hunter, P. A. Bullough, EMBO J. 2004, 23, 690-700
A. M. Seddon, P. Curnow, P. J. Booth, Biochim. Biophys. Acta Biomembr. 2004, 1666, 105-117
C. Ostermeier, H. Michel, Curr. Opin. Struct. Biol. 1997, 7, 697-701.
M. C. Wiener, Curr. Opin. Colloid Interface Sci. 2001, 6, 412-419.
P. D. Laible, C. Kirmaier, C. S. Udawatte, S. J. Hofman, D. Holten, D. K. Hanson, Biochemistry 2003, 42, 1718-1730
X. Zhao, Y. Nagai, P. J. Reeves, P. Kiley, H. G. Khorana, S. Zhang, Proc. Natl. Acad. Sci. USA 2006, 103, 17707-17712
2004; 81
2000; 9
2002; 35
2004; 23
1966; 70
1993; 262
1992 1992; 104 31
2005; 88
2004; 1666
2005; 1751
1997; 7
1994; 42
2001; 276
2005; 280
2006; 63
2001; 6
1999; 18
1980; 77
2004; 13
2007; 41
2001; 11
2007 2007; 119 46
2003; 302
2000 2000; 112 39
2003; 42
2003; 21
2003; 142
2006; 103
17013556 - Cell Mol Life Sci. 2006 Nov;63(22):2597-607
11432878 - J Biol Chem. 2001 Aug 31;276(35):32403-6
12578387 - Biochemistry. 2003 Feb 18;42(6):1718-30
17098868 - Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17707-12
24306562 - Photosynth Res. 1994 Dec;42(3):203-15
15963773 - Biochim Biophys Acta. 2005 Aug 10;1751(2):213-6
12718924 - J Struct Biol. 2003 Apr;142(1):108-32
6935651 - Proc Natl Acad Sci U S A. 1980 Nov;77(11):6368-70
9345629 - Curr Opin Struct Biol. 1997 Oct;7(5):697-701
Chembiochem. 2013 Apr 15;14(6):665
12718926 - J Struct Biol. 2003 Apr;142(1):144-53
11495729 - Curr Opin Struct Biol. 2001 Aug;11(4):397-402
15215534 - Protein Sci. 2004 Jul;13(7):1948-9
12524549 - Nat Biotechnol. 2003 Feb;21(2):171-6
8235592 - Science. 1993 Oct 29;262(5134):734-8
17367711 - Methods. 2007 Apr;41(4):388-97
15519311 - Biochim Biophys Acta. 2004 Nov 3;1666(1-2):105-17
14671305 - Science. 2003 Dec 12;302(5652):1969-72
17691085 - Angew Chem Int Ed Engl. 2007;46(37):7023-5
11206073 - Protein Sci. 2000 Dec;9(12):2518-27
11997980 - Q Rev Biophys. 2002 Feb;35(1):1-62
16034527 - Photosynth Res. 2004;81(3):207-14
15522874 - J Biol Chem. 2005 Jan 14;280(2):1426-31
15652249 - Prog Biophys Mol Biol. 2005 Jul;88(3):339-57
10760860 - Angew Chem Int Ed Engl. 2000 Feb;39(4):758-761
14765115 - EMBO J. 2004 Feb 25;23(4):690-700
9927413 - EMBO J. 1999 Feb 1;18(3):534-42
e_1_2_4_20_2
e_1_2_4_22_2
e_1_2_4_21_2
e_1_2_4_24_2
e_1_2_4_22_3
e_1_2_4_23_2
e_1_2_4_26_2
e_1_2_4_25_2
e_1_2_4_28_2
e_1_2_4_26_3
e_1_2_4_27_2
e_1_2_4_29_2
e_1_2_4_2_2
e_1_2_4_1_2
e_1_2_4_4_2
e_1_2_4_3_2
e_1_2_4_6_2
e_1_2_4_5_2
e_1_2_4_8_2
e_1_2_4_7_2
e_1_2_4_9_2
e_1_2_4_31_2
e_1_2_4_30_2
e_1_2_4_10_2
e_1_2_4_33_2
e_1_2_4_11_2
e_1_2_4_32_2
e_1_2_4_12_2
e_1_2_4_35_2
e_1_2_4_13_2
e_1_2_4_34_2
e_1_2_4_14_2
e_1_2_4_37_2
e_1_2_4_15_2
e_1_2_4_36_2
e_1_2_4_16_3
e_1_2_4_17_2
e_1_2_4_16_2
e_1_2_4_38_2
e_1_2_4_19_2
e_1_2_4_18_2
References_xml – volume: 112 39
  start-page: 774 758
  year: 2000 2000
  end-page: 777 761
  publication-title: Angew. Chem. Angew. Chem. Int. Ed.
– volume: 276
  start-page: 32403
  year: 2001
  end-page: 32406
  publication-title: J. Biol. Chem.
– volume: 13
  start-page: 1948
  year: 2004
  end-page: 1949
  publication-title: Protein Sci.
– volume: 262
  start-page: 734
  year: 1993
  end-page: 738
  publication-title: Science
– volume: 18
  start-page: 534
  year: 1999
  end-page: 542
  publication-title: EMBO J.
– volume: 119 46
  start-page: 1756 1726
  year: 2007 2007
  end-page: 1759 1729
  publication-title: Angew. Chem. Angew. Chem. Int. Ed.
– volume: 142
  start-page: 108
  year: 2003
  end-page: 132
  publication-title: J. Struct. Biol.
– volume: 1666
  start-page: 105
  year: 2004
  end-page: 117
  publication-title: Biochim. Biophys. Acta Biomembr.
– volume: 35
  start-page: 1
  year: 2002
  end-page: 62
  publication-title: Quart. Rev. Biophys.
– volume: 302
  start-page: 1969
  year: 2003
  end-page: 1972
  publication-title: Science
– volume: 23
  start-page: 690
  year: 2004
  end-page: 700
  publication-title: EMBO J.
– volume: 88
  start-page: 339
  year: 2005
  end-page: 357
  publication-title: Prog. Biophys. Mol. Biol.
– volume: 63
  start-page: 2597
  year: 2006
  end-page: 2607
  publication-title: Cell. Mol. Life Sci.
– volume: 6
  start-page: 412
  year: 2001
  end-page: 419
  publication-title: Curr. Opin. Colloid Interface Sci.
– volume: 280
  start-page: 1426
  year: 2005
  end-page: 1431
  publication-title: J. Biol. Chem.
– volume: 104 31
  start-page: 1147 1124
  year: 1992 1992
  end-page: 1157 1134
  publication-title: Angew. Chem. Angew. Chem. Int. Ed. Engl.
– volume: 77
  start-page: 6368
  year: 1980
  end-page: 6370
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 7
  start-page: 697
  year: 1997
  end-page: 701
  publication-title: Curr. Opin. Struct. Biol.
– volume: 11
  start-page: 397
  year: 2001
  end-page: 402
  publication-title: Curr. Opin. Struct. Biol.
– volume: 81
  start-page: 207
  year: 2004
  end-page: 214
  publication-title: Photosynth. Res.
– volume: 42
  start-page: 203
  year: 1994
  end-page: 215
  publication-title: Photosynth. Res.
– volume: 41
  start-page: 388
  year: 2007
  end-page: 397
  publication-title: Methods
– volume: 103
  start-page: 17707
  year: 2006
  end-page: 17712
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 42
  start-page: 1718
  year: 2003
  end-page: 1730
  publication-title: Biochemistry
– volume: 21
  start-page: 171
  year: 2003
  end-page: 176
  publication-title: Nat. Biotechnol.
– volume: 142
  start-page: 144
  year: 2003
  end-page: 153
  publication-title: J. Struct. Biol.
– volume: 1751
  start-page: 213
  year: 2005
  end-page: 216
  publication-title: Biochim. Biophys. Acta Proteins Proteomics
– volume: 70
  start-page: 2966
  year: 1966
  end-page: 2973
  publication-title: J. Phys. Chem.
– volume: 9
  start-page: 2518
  year: 2000
  end-page: 2527
  publication-title: Protein Sci.
– ident: e_1_2_4_13_2
  doi: 10.1074/jbc.R100031200
– ident: e_1_2_4_25_2
  doi: 10.1016/S0959-440X(97)80080-2
– ident: e_1_2_4_33_2
– ident: e_1_2_4_35_2
  doi: 10.1093/emboj/18.3.534
– ident: e_1_2_4_22_3
  doi: 10.1002/(SICI)1521-3773(20000218)39:4<758::AID-ANIE758>3.0.CO;2-V
– ident: e_1_2_4_7_2
  doi: 10.1016/S0959-440X(00)00223-2
– ident: e_1_2_4_10_2
  doi: 10.1007/s00018-006-6252-y
– ident: e_1_2_4_34_2
  doi: 10.1126/science.1088892
– ident: e_1_2_4_5_2
– ident: e_1_2_4_3_2
  doi: 10.1016/S1047-8477(03)00045-5
– ident: e_1_2_4_18_2
  doi: 10.1038/nbt776
– ident: e_1_2_4_21_2
– ident: e_1_2_4_11_2
  doi: 10.1016/j.ymeth.2007.01.007
– ident: e_1_2_4_38_2
– ident: e_1_2_4_4_2
  doi: 10.1110/ps.04712004
– ident: e_1_2_4_6_2
– ident: e_1_2_4_19_2
  doi: 10.1126/science.8235592
– ident: e_1_2_4_2_2
– ident: e_1_2_4_30_2
  doi: 10.1021/bi026959b
– ident: e_1_2_4_8_2
  doi: 10.1016/j.bbamem.2004.04.011
– ident: e_1_2_4_1_2
  doi: 10.1016/S1047-8477(03)00043-1
– ident: e_1_2_4_27_2
– ident: e_1_2_4_9_2
  doi: 10.1016/j.pbiomolbio.2004.07.006
– ident: e_1_2_4_36_2
  doi: 10.1038/sj.emboj.7600092
– ident: e_1_2_4_12_2
– ident: e_1_2_4_20_2
  doi: 10.1073/pnas.77.11.6368
– ident: e_1_2_4_23_2
  doi: 10.1110/ps.9.12.2518
– ident: e_1_2_4_14_2
  doi: 10.1016/S1359-0294(01)00107-8
– ident: e_1_2_4_32_2
  doi: 10.1021/j100881a041
– ident: e_1_2_4_16_2
  doi: 10.1002/ange.200604276
– ident: e_1_2_4_29_2
  doi: 10.1023/B:PRES.0000036883.56959.a9
– ident: e_1_2_4_37_2
  doi: 10.1074/jbc.M411334200
– ident: e_1_2_4_15_2
– ident: e_1_2_4_26_3
  doi: 10.1002/anie.199211241
– ident: e_1_2_4_26_2
  doi: 10.1002/ange.19921040905
– ident: e_1_2_4_28_2
  doi: 10.1017/S0033583501003754
– ident: e_1_2_4_17_2
  doi: 10.1073/pnas.0607167103
– ident: e_1_2_4_31_2
  doi: 10.1007/BF00018263
– ident: e_1_2_4_24_2
  doi: 10.1016/j.bbapap.2005.04.011
– ident: e_1_2_4_22_2
  doi: 10.1002/(SICI)1521-3757(20000218)112:4<774::AID-ANGE774>3.0.CO;2-W
– ident: e_1_2_4_16_3
  doi: 10.1002/anie.200604276
SSID ssj0009631
Score 2.169457
Snippet Three‐legged friends: Intrinsic membrane proteins must usually be extracted from the native membrane with the aid of synthetic amphiphiles and then stabilized...
Intrinsic membrane proteins must usually be extracted from the native membrane with the aid of synthetic amphiphiles and then stabilized in a soluble form...
SourceID pubmedcentral
proquest
crossref
pubmed
wiley
istex
fao
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 1706
SubjectTerms amphiphiles
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Carbohydrates - chemical synthesis
Carbohydrates - chemistry
Carbohydrates - pharmacology
Cell Membrane - chemistry
Cell Membrane - metabolism
detergent design
membrane proteins
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Protein Binding - drug effects
Rhodobacter capsulatus - chemistry
Solubility - drug effects
solubilization
stabilization
Title Glycotripod Amphiphiles for Solubilization and Stabilization of a Membrane-Protein Superassembly: Importance of Branching in the Hydrophilic Portion
URI https://api.istex.fr/ark:/67375/WNG-33FQ0PKN-Z/fulltext.pdf
https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcbic.200800169
https://www.ncbi.nlm.nih.gov/pubmed/18576450
https://search.proquest.com/docview/69316674
https://pubmed.ncbi.nlm.nih.gov/PMC3527902
Volume 9
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3LbtQwFLWgG9jwaIGGpxeorNImthMn7KYD0ymIUaFUVGws23HKqDNJNQ-JYcUnsOID-RLudTovQEICKZtJ4oztHDvn2sfHhDwtcq3hbZpQp0xAgCKgSeUsDV1WyMgxy6z1Atle2j0Rr06T05VV_I0_xGLADVuG76-xgWsz3luahlrT9xaEGbIWXMEXc4marhfvlv5RgC4fcQmc7mRMzl0bI7a3nnztq3S11DVwVazmz38inr_rJ1d5rf8wdW4SPS9So0c5351OzK798ovb4_-U-Ra5cclaaauB2W1yxVWbZKtVQcQ-nNEd6nWkfoB-k1xrz_eQ2yLfDwYzW0-gY6oL2gLg9OEYuDEFqkxxRA6luc1CUKqrggL1XTlTl1TTN24IOa7cj6_fjtBTol_R4-mFGwHphyuD2XN6OPQxBKAXU-zjTiE4rEbhTmC3tDsrRjX-bd9S1M3Ck--Qk87L9-1ueLkTRGiBbuYhkFSrXcqKKDWJzUXOY6YdMzjJmhWGO-YyY3hcyDi2pSxZlDDD8iS33MXClPwu2ajqym0TypO8EIKXQNe5yJwEZOYZrqflLLLWRQF5NkeCumgMP1Rj7cwUVr9aVH9AtgEoSp9Bb6xOjhnOAQP7SoHTBmTHo2fxBD06RwWdTNSH3oHivPM2OnrdUx8D8mQOLwWvBidroEbr6VilUMY0lSIg9xqwLXOTQYwoEsipXIPh4gZ0DF-_UvU_eedwYNsyj1hAmEfZXwqo2vuH7cWv-_-S6AG53ghsZMjih2RjMpq6R8DiJuaxb6k_ASSlQFE
link.rule.ids 230,315,783,787,888,1378,27938,27939,46308,46732
linkProvider Wiley-Blackwell
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwFLbYeBgvXDZg4TY_oPGULbFzM29doWvZVg22iokXK3YcqNYmU9dKlCd-Ak_8QH4J5zhLugISEkh5aRKntvMd5zvHx58JeZ6JNIW3qdw0YgE4KAGYlGCRa5Is9gzTTGubINuPuoPgzVlYZxPiWphKH6IJuKFl2PEaDRwD0rsL1VCthlaDMEHaIlbITbB5jpsYvHq3UJACfFmfK8AJT8biWrfRY7vL5Ze-Syt5WgJbxY7-_Cfq-XsG5XVmaz9NnTtE1Y2qMlLOd2ZTtaO__KL3-F-tvktuXxFX2qqQdo_cMMU62WgV4LSP53Sb2lRSG6NfJ2vtehu5DfJ9fzTX5RTGpjKjLcDOEI6RuaTAlikG5TA7t1oLStMio8B-r50pc5rSIzOGKhfmx9dvxygrMSzoyezCTID3w5XR_CXtja0bAQDGEnu4WQhG1ijcCQSXdufZpMS_HWqKqbPw5Ptk0Hl92u66V5tBuBoYp3CBp-rURCzzIhVqEQjus9QwhfOsSaa4YSZRivtZ7Ps6j3PmhUwxEQrNjR-onD8gq0VZmE1CeSiyIOA5MHYeJCYGcIoEl9Ry5mltPIe8qKEgLyrND1mpOzOJ3S-b7nfIJiBFph9hQJaDE4bTwEDAIqC1Dtm28GmekE7OMYkuDuX7_r7kvPPWOz7oyw8O2arxJeHV4HwN9Gg5u5QRtDGK4sAhDyu0LWqTgJsYhFDTeAmHzQ0oGr58pRh-suLhQLhj4TGHMAuzvzRQtvd67ebXo38ptEXWuqdHh_Kw1z94TG5V-Taxy_wnZHU6mZmnQOqm6pk125__zURr
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwGLXYkIAXLhuwcJsf0HjKltjOxbx1HV3LoCqMiokXy3YcqNYmVddKlCd-Ak_8QH4Jn52lF0BCAikvTeLUdo6d89nHxwg9zbiU8DaVL2PCIEBh0KQ4iX2TZklgiCZaO4FsN2732cuz6GxlFX_lD7EYcLMtw_XXtoGPs_xgaRqq1cBZEKaWtfANdJXFNLCirqO3SwMpgJcLuZid7yQkqW0bA3Kwnn7ts7SRyxLIqq3nz39inr8LKFeJrfsytW4hWZepEqSc78-mal9_-cXu8X8KfRvdvKStuFHh7A66YoottN0oIGQfzfEedkJSN0K_ha43603kttH34-Fcl1PomcoMNwA5AziG5gIDV8Z2SM5qc6uVoFgWGQbuu3KmzLHEr80IclyYH1-_9aypxKDAp7OxmQDrhyvD-XPcGbkgAuBrUxzarULsuBqGO4He4vY8m5T2bwcaW-EsPPku6rdevGu2_cutIHwNfJP7wFK1NDHJglhFmjNOQyINUXaWNc0UNcSkStEwS8JQ50lOgogowiOuqQmZyuk9tFmUhdlBmEY8Y4zmwNcpS00C0OSpXVBLSaC1CTz0rEaCGFeOH6LydibCVr9YVL-HdgAoQn6E7lj0T4mdBAb6FQOp9dCeQ8_iCXJybiV0SSTed48Fpa03Qe-kKz54aLeGl4BXY2droEbL2YWIoYxxnDAP3a_AtsxNCkEiiyCnyRoMFzdYy_D1K8Xgk7MOB7qd8IB4iDiU_aWAonnYaS5-PfiXRLvoWu-oJV51uicP0Y1KbJP4JHyENqeTmXkMjG6qnrhG-xP9S0Ma
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Glycotripod+amphiphiles+for+solubilization+and+stabilization+of+a+membrane-protein+superassembly%3A+importance+of+branching+in+the+hydrophilic+portion&rft.jtitle=Chembiochem+%3A+a+European+journal+of+chemical+biology&rft.au=Chae%2C+Pil+S&rft.au=Wander%2C+Marc+J&rft.au=Bowling%2C+Aaron+P&rft.au=Laible%2C+Philip+D&rft.date=2008-07-21&rft.eissn=1439-7633&rft.volume=9&rft.issue=11&rft.spage=1706&rft_id=info:doi/10.1002%2Fcbic.200800169&rft_id=info%3Apmid%2F18576450&rft.externalDocID=18576450
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1439-4227&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1439-4227&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1439-4227&client=summon