集成免疫磁珠富集和免疫层析的黄曲霉毒素M1快速检测法
以喷涂了检测抗原黄曲霉毒素M1-BSA和驴抗鼠二抗形成检测线和质控线的硝酸纤维膜制备免疫层析试纸条,采用EDC/NHS法制备偶联了抗黄曲霉毒素M1单克隆抗体的免疫磁珠。免疫磁珠与待检样本混合,经捕获、磁分离后,浓缩重悬液直接用免疫层析试纸条检测,首次建立了集浓缩样本与免疫层析于一体的黄曲霉毒素M1快速检测法。该方法用于检测原料乳中黄曲霉毒素M1,检出限为0.1μg/L,低于我国制定的黄曲霉毒素M1限量标准（0.5μg/L）,与其它真菌毒素和原料乳中常检违法添加物无交叉反应,分析结果与酶联免疫吸附法（ ELISA）结果一致。本方法适合现场快速检测原料乳中黄曲霉毒素M1。...
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| Published in | 分析化学 Vol. 42; no. 5; pp. 654 - 659 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
南昌大学食品科学与技术国家重点实验室,南昌,330047%江西中德生物工程有限公司,南昌,330047
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2014.30902 |
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| Abstract | 以喷涂了检测抗原黄曲霉毒素M1-BSA和驴抗鼠二抗形成检测线和质控线的硝酸纤维膜制备免疫层析试纸条,采用EDC/NHS法制备偶联了抗黄曲霉毒素M1单克隆抗体的免疫磁珠。免疫磁珠与待检样本混合,经捕获、磁分离后,浓缩重悬液直接用免疫层析试纸条检测,首次建立了集浓缩样本与免疫层析于一体的黄曲霉毒素M1快速检测法。该方法用于检测原料乳中黄曲霉毒素M1,检出限为0.1μg/L,低于我国制定的黄曲霉毒素M1限量标准（0.5μg/L）,与其它真菌毒素和原料乳中常检违法添加物无交叉反应,分析结果与酶联免疫吸附法（ ELISA）结果一致。本方法适合现场快速检测原料乳中黄曲霉毒素M1。 |
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| AbstractList | 以喷涂了检测抗原黄曲霉毒素M1-BSA和驴抗鼠二抗形成检测线和质控线的硝酸纤维膜制备免疫层析试纸条,采用EDC/NHS法制备偶联了抗黄曲霉毒素M1单克隆抗体的免疫磁珠。免疫磁珠与待检样本混合,经捕获、磁分离后,浓缩重悬液直接用免疫层析试纸条检测,首次建立了集浓缩样本与免疫层析于一体的黄曲霉毒素M1快速检测法。该方法用于检测原料乳中黄曲霉毒素M1,检出限为0.1μg/L,低于我国制定的黄曲霉毒素M1限量标准(0.5μg/L),与其它真菌毒素和原料乳中常检违法添加物无交叉反应,分析结果与酶联免疫吸附法( ELISA)结果一致。本方法适合现场快速检测原料乳中黄曲霉毒素M1。 以喷涂了检测抗原黄曲霉毒素M1-BSA和驴抗鼠二抗形成检测线和质控线的硝酸纤维膜制备免疫层析试纸条,采用EDC/NHS法制备偶联了抗黄曲霉毒素M1单克隆抗体的免疫磁珠。免疫磁珠与待检样本混合,经捕获、磁分离后,浓缩重悬液直接用免疫层析试纸条检测,首次建立了集浓缩样本与免疫层析于一体的黄曲霉毒素M1快速检测法。该方法用于检测原料乳中黄曲霉毒素M1,检出限为0.1μg/L,低于我国制定的黄曲霉毒素M1限量标准（0.5μg/L）,与其它真菌毒素和原料乳中常检违法添加物无交叉反应,分析结果与酶联免疫吸附法（ ELISA）结果一致。本方法适合现场快速检测原料乳中黄曲霉毒素M1。 |
| Abstract_FL | Immunochromatographic strip was assembled with conjugate pad, sample pad, absorbent pad, and the nitrocellulose membrane on which aflatoxin M1-BSA and donkey anti-mouse antibody was sprayed and served as test line and control line, respectively. Anti-aflatoxin M1 coated immunomagnetic nanobeads was synthesized by an EDC/NHS method. Immunomagnetic nanobeads were mixed with sample for capturing and separation. The enrichment sample was detected by immunochromatographic strip. A rapid detection method of aflatoxin M1 containing sample enrichment and immunochromatographic assay was firstly set up. The limit of detection of this assay for aflatoxin M1 in raw milk was 0. 1 μg/L, which was lower than the legal limit of 0. 5 μg/L set by China. No cross-reactions were found with other mycotoxins and common illegal additives. The result of the method was in good agreement with that of ELISA. The assay can be applied for fast detection of aflatoxin M1 on-site in raw milk. |
| Author | 黄艳梅 刘道峰 赖卫华 熊勇华 杨万春 刘坤 王树颖 |
| AuthorAffiliation | 南昌大学食品科学与技术国家重点实验室,南昌330047 江西中德生物工程有限公司,南昌330047 |
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| Author_FL | YANG Wan-Chun WANG Shu-Ying LIU Kun XIONG Yong-Hua HUANG Yan-Mei LIU Dao-Feng LAI Wei-Hua |
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| Keywords | 免疫磁珠 Enrichment Raw milk 富集 Aflatoxin M1 Immunochromatography 免疫层析 Immunomagnetic nanobeads 黄曲霉毒素M1 原料乳 |
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| Notes | HUANG Yan-Mei, LIU Dao-Feng, LAI Wei-Hua, XIONG Yong-Hua, YANG Wan-Chun, LIU Kun, WANG Shu-Ying 1（ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China） 2（ Jiangxi Zodolabs Biotech Co. I_zd, Nanchang 330047, China） Ailatoxin M1; Immunomagnetic nanobeads; Enrichment; Immunochromatography; Raw milk Immunochromatographic strip was assembled with conjugate pad, sample pad, absorbent pad, and the nitrocellulose membrane on which aflatoxin M1-BSA and donkey anti-mouse antibody was sprayed and served as test line and control line, respectively. Anti-aflatoxin M1 coated immunomagnetic nanobeads was synthesized by an EDC/NHS method. Immunomagnetic nanobeads were mixed with sample for capturing and separation. The enrichment sample was detected by immunochromatographic strip. A rapid detection method of aflatoxin M1 containing sample enrichment and immunochromatographic assay was firstly set up. The limit of detection of this assay for aflatoxin M1 in raw milk was 0. 1 μg/L, wh |
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| Publisher | 南昌大学食品科学与技术国家重点实验室,南昌,330047%江西中德生物工程有限公司,南昌,330047 |
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| SubjectTerms | 免疫层析 免疫磁珠 原料乳 富集 黄曲霉毒素M1 |
| Title | 集成免疫磁珠富集和免疫层析的黄曲霉毒素M1快速检测法 |
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