基于二元混合自组装包裹纳米颗粒的微传感器抗体固定方法
采用二元混合自组装膜修饰纳米金颗粒,在经自组装单分子层修饰的金电极上阵列式排布,并通过共价键固定抗体形成生物敏感膜。采用原子力显微镜、扫描电镜和阻抗谱分别对电极表面的修饰过程进行了表征。纳米粒子在微电极表面均匀分布,没有明显的团聚,并且可实现抗体有效固定。基于标准互补金属氧化物半导体（Complementary metal oxide semiconductor,CMOS）工艺和微加工技术,利用该抗体固定化方法,制备了糖化血红蛋白免疫微传感器,可同时检测血液中的糖化血红蛋白和血红蛋白含量,其对糖化血红蛋白和血红蛋白的检测范围分别为14～170μg/L和167～570μg/L。...
Saved in:
| Published in | 分析化学 Vol. 39; no. 6; pp. 804 - 808 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国科学院研究生院,北京,100080%中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190
2011
中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2011.00804 |
Cover
| Abstract | 采用二元混合自组装膜修饰纳米金颗粒,在经自组装单分子层修饰的金电极上阵列式排布,并通过共价键固定抗体形成生物敏感膜。采用原子力显微镜、扫描电镜和阻抗谱分别对电极表面的修饰过程进行了表征。纳米粒子在微电极表面均匀分布,没有明显的团聚,并且可实现抗体有效固定。基于标准互补金属氧化物半导体（Complementary metal oxide semiconductor,CMOS）工艺和微加工技术,利用该抗体固定化方法,制备了糖化血红蛋白免疫微传感器,可同时检测血液中的糖化血红蛋白和血红蛋白含量,其对糖化血红蛋白和血红蛋白的检测范围分别为14～170μg/L和167～570μg/L。 |
|---|---|
| AbstractList | 采用二元混合自组装膜修饰纳米金颗粒,在经自组装单分子层修饰的金电极上阵列式排布,并通过共价键固定抗体形成生物敏感膜。采用原子力显微镜、扫描电镜和阻抗谱分别对电极表面的修饰过程进行了表征。纳米粒子在微电极表面均匀分布,没有明显的团聚,并且可实现抗体有效固定。基于标准互补金属氧化物半导体（Complementary metal oxide semiconductor,CMOS）工艺和微加工技术,利用该抗体固定化方法,制备了糖化血红蛋白免疫微传感器,可同时检测血液中的糖化血红蛋白和血红蛋白含量,其对糖化血红蛋白和血红蛋白的检测范围分别为14～170μg/L和167～570μg/L。 O646.54; 采用二元混合自组装膜修饰纳米金颗粒,在经自组装单分子层修饰的金电极上阵列式排布,并通过共价键固定抗体形成生物敏感膜.采用原子力显微镜、扫描电镜和阻抗谱分别对电极表面的修饰过程进行了表征.纳米粒子在微电极表面均匀分布,没有明显的团聚,并且可实现抗体有效固定.基于标准互补金属氧化物半导体(Complementary metal oxide semiconductor,CMOS)工艺和微加工技术,利用该抗体固定化方法,制备了糖化血红蛋白免疫微传感器,可同时检测血液中的糖化血红蛋白和血红蛋白含量,其对糖化血红蛋白和血红蛋白的检测范围分别为14～170μg/L和167～570μg/L. |
| Author | 薛茜男 边超 佟建华 孙楫舟 张虹 夏善红 |
| AuthorAffiliation | 中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京100190 中国科学院研究生院,北京100080 |
| AuthorAffiliation_xml | – name: 中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190;中国科学院研究生院,北京,100080%中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190 |
| Author_FL | SUN Ji-Zhou XIA Shan-Hong BIAN Chao XUE Qian-Nan ZHANG Hong TONG Jian-Hua |
| Author_FL_xml | – sequence: 1 fullname: XUE Qian-Nan – sequence: 2 fullname: BIAN Chao – sequence: 3 fullname: TONG Jian-Hua – sequence: 4 fullname: SUN Ji-Zhou – sequence: 5 fullname: ZHANG Hong – sequence: 6 fullname: XIA Shan-Hong |
| Author_xml | – sequence: 1 fullname: 薛茜男 边超 佟建华 孙楫舟 张虹 夏善红 |
| BookMark | eNotj0tLAmEYhb-FQVb-gzYtWs70fvfPZUhXpILcy8w4o0aNpUS2TilCIoqSbGHkJloI5UKcLr9mLvYvmrDVWZyH53BmUMKtuDZC8xh0Kglb2t3RN3UMaaETwFgHUMASKAmEU40qAtMoVauVTYhLYIqrJNoKup7vXfleK2iehcNhcH0xPn-NPhrjXjNoNce9UeQNorfBz3M7er-JOo3gu-9_PoWNbvDwEl62_a_b4NEL-p3wfhQO7ubQlGPs1-zUf86i3OpKLrOuZbfXNjLLWc3iQmgUF6QjqTBNTkBxYIyALRxaUBYBDpaNiSI0bWLOuWQ4zaUA4QjTkWlDUm7TWbQ40Z4YrmO4xfxe5bjqxoN5p16q_30HASBibmHCWaWKWzwqx-RhtXxgVE_zVGHJKDD6C4Opdsc |
| ClassificationCodes | O646.54 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3724/SP.J.1096.2011.00804 |
| DatabaseName | 中文科技期刊数据库 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| DocumentTitleAlternate | Micro Biosensor Antibody Immobilization Method Based on Mixed Self-assembly-monolayers Wrapped Nano-particle |
| DocumentTitle_FL | Micro Biosensor Antibody Immobilization Method Based on Mixed Self-assembly-monolayers Wrapped Nano-particle |
| EndPage | 808 |
| ExternalDocumentID | fxhx201106006 38174304 |
| GrantInformation_xml | – fundername: 国家973计划项目; 国家863计划项目 funderid: (2009CB320300); (2006AA04Z366) |
| GroupedDBID | --K --M .~1 0R~ 1B1 1~. 1~5 29B 2B. 2C. 2RA 4.4 457 4G. 5GY 5VS 7-5 71M 8P~ 92E 92I 92L AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AARLI AAXUO ABFNM ABMAC ABXDB ABYKQ ACDAQ ACGFS ACNNM ACRLP ADBBV ADECG ADEZE ADMUD AEBSH AEKER AENEX AFKWA AFTJW AFZHZ AGHFR AGUBO AGYEJ AIEXJ AIKHN AITUG AJBFU AJOXV AJSZI ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ AXJTR BKOJK BLXMC CQIGP CS3 CW9 DU5 EBS EFJIC EFLBG EJD EO9 EP2 EP3 FDB FEDTE FIRID FLBIZ FNPLU FYGXN GBLVA HVGLF HZ~ J1W KOM M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 ROL SDF SDG SES SPC SPCBC SSK SSZ T5K TCJ TGP U1G U5L ~G- ~WA -02 -0B -SB -S~ .5D 188 4A8 5VR 5XA 5XC 8RM 92M 93N 9D9 9DB AAITT AATTM AAXKI AAYWO ABJNI ABWVN ACRPL ADNMO ADVLN AEIPS AFJKZ AFUIB AFXIZ AGCQF AGRNS AIIUN AKRWK ANKPU APXCP BBR BNPGV CAJEB CCEZO CDRFL CDYEO CJPJV CW7 FA0 J.9 JUIAU M~T PB1 PB6 PSX Q-- R-B RT2 S.. SSH T8R U1F U5B UY8 UZ4 W94 ~LN |
| ID | FETCH-LOGICAL-c566-31d7f736bb5208504420e6f3d8c2050ce128239b1555741957606f6bf79a735e3 |
| ISSN | 0253-3820 |
| IngestDate | Thu May 29 04:00:21 EDT 2025 Wed Feb 14 09:53:33 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Keywords | 集成芯片 微加工 纳米金 互补金属氧化物半导体 糖化血红蛋白 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c566-31d7f736bb5208504420e6f3d8c2050ce128239b1555741957606f6bf79a735e3 |
| Notes | Nano-gold particles that wrapped by mixed self-assembly monolayers（SAM） were immobilized on the SAM-modified Au micro electrode surface by amide linkage to form nano-particles array.Subsequently,a sensitive bio-film on the micro immunosensor surface was formed with covalent immobilization of antibodies on the nano-particles.Micro electrode surface modification process was characterized by atomic force microscopy, scanning electron microscope,and electrochemical impe-dance spectroscopy.The results indicated that the nano-particles distributed uniformly without appa-rent aggregation and the antibodies can be effectively immobilized on the microelectrode surface.Using this antibody immobilization method,hemoglobin-A1c（HbAIc） micro-sensors were prepared.Based on complementary metal-oxide-semiconductor（CMOS） standard process and micro-electronic-mechanical system（MEMS） technology,the immunosensor can detect hemoglobin-A1c and hemoglobin simultaneously with the range of 166.7-570 μg/L hemoglobin and 13.75-170.5 μg/ |
| PageCount | 5 |
| ParticipantIDs | wanfang_journals_fxhx201106006 chongqing_primary_38174304 |
| PublicationCentury | 2000 |
| PublicationDate | 2011 |
| PublicationDateYYYYMMDD | 2011-01-01 |
| PublicationDate_xml | – year: 2011 text: 2011 |
| PublicationDecade | 2010 |
| PublicationTitle | 分析化学 |
| PublicationTitleAlternate | Chinese Journal of Analytical Chemistry |
| PublicationTitle_FL | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY |
| PublicationYear | 2011 |
| Publisher | 中国科学院研究生院,北京,100080%中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190 中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190 |
| Publisher_xml | – name: 中国科学院研究生院,北京,100080%中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190 – name: 中国科学院电子学研究所传感技术联合国家重点实验室北方基地,北京,100190 |
| SSID | ssib001104858 ssib048394981 ssj0037577 ssib002258198 ssib007686865 ssib051370160 |
| Score | 1.8753319 |
| Snippet | 采用二元混合自组装膜修饰纳米金颗粒,在经自组装单分子层修饰的金电极上阵列式排布,并通过共价键固定抗体形成生物敏感膜。采用原子力显微镜、扫描电镜和阻抗谱分别对电极表面的修饰过程进行了表征。纳米粒子在微电极表面均匀分布,没有明显的团聚,并且可实现抗体有效固定。基于标准互补金属氧化物半导体（Complementary... O646.54;... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 804 |
| SubjectTerms | 互补金属氧化物半导体 微加工 糖化血红蛋白 纳米金 集成芯片 |
| Title | 基于二元混合自组装包裹纳米颗粒的微传感器抗体固定方法 |
| URI | http://lib.cqvip.com/qk/93919X/201106/38174304.html https://d.wanfangdata.com.cn/periodical/fxhx201106006 |
| Volume | 39 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NT9RAFJ8gHvRi_IygEg5OPJBi6bTzcZwuJYQD8YAJt822tHBaUCEhHLwI0RhijEaJeMDIxXggUQ6E9eOv2e7if-F7M-1SIzFq0jRvX997897MduY3becNITdTnmRczEkHoHLm-KmrHJBsOAmXSRa7DZwj4NcW03zyrj81G8z2nbpV-WppZTkeTdZOXFfyP60KPGhXXCX7Dy3bMwoMoKF94QwtDOe_amMaBVRN0FDTyMezjEqihpckHIxGnIYCDxR2qZQ0klQKqkEL-CGVPnI0Q3nUqhnCcEJlZDQNmSHGDKGo9qgShgOEh4TSxk5Aw4hq60aNahdLBz44iaWDojQcbdRBZpwqZi6FJooAdcEUyChuSudYot0gs8TQxkkIgRuxCIOyboMKWhinuve4EeMANpjHoEGkZpyFksUIssBbLAUI8CoYKX0y7oalT3IcanaksK2MUxpoaxPsT5hrRbwSJUJlxQGo28hNLdiq1F71SUsxBtiu2AuYw6TnVscNm4SpuD-qg4C0GyqXeMLkrfhtqGLC8_Fl-Z3RKUzpxYtssm5P-Zck4JhH0WeY9_a0J8QYfrs6-rCatw864srrbeik4SY7_g1zSi6PX2f7gIp9dbzgOBhjAjMNlhCGicBsWdoL2645RZdvn-Qw5iVZWGzO3wO0ZRa_NbNGc76C02bOk3PFBGtY27vlAulbW7hIztTKfQ0vkel8p9VuPWu3NvONR52Dg_z5k6PHH7tf1o92N_LNjaPdw25rv_tp_8f7re7nF93t9fz7Xvvru876Tv7mQ-fpVvvby_xtK9_b7rw-7Oy_ukxmJqKZ2qRT7CriJDB1AcwxJzLBeBwHuD2t6_uem_KMzcnEcwM3SQGweUzFgLMDQNsK5uMuz3icCdUQLEjZFdLfXGymV8kwYF0mEi8GuMD9OBUNmcF0S7rcbQjup_4AGexVS33JJo-ply05QIaKeqoXPcqDera6sGoAOcxC-OCftK-Rs_blBh7XSf_y_ZX0BqDj5XjI_DN-ArhUkBk |
| linkProvider | Elsevier |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%9F%BA%E4%BA%8E%E4%BA%8C%E5%85%83%E6%B7%B7%E5%90%88%E8%87%AA%E7%BB%84%E8%A3%85%E5%8C%85%E8%A3%B9%E7%BA%B3%E7%B1%B3%E9%A2%97%E7%B2%92%E7%9A%84%E5%BE%AE%E4%BC%A0%E6%84%9F%E5%99%A8%E6%8A%97%E4%BD%93%E5%9B%BA%E5%AE%9A%E6%96%B9%E6%B3%95&rft.jtitle=%E5%88%86%E6%9E%90%E5%8C%96%E5%AD%A6&rft.au=%E8%96%9B%E8%8C%9C%E7%94%B7+%E8%BE%B9%E8%B6%85+%E4%BD%9F%E5%BB%BA%E5%8D%8E+%E5%AD%99%E6%A5%AB%E8%88%9F+%E5%BC%A0%E8%99%B9+%E5%A4%8F%E5%96%84%E7%BA%A2&rft.date=2011&rft.issn=0253-3820&rft.volume=39&rft.issue=6&rft.spage=804&rft.epage=808&rft_id=info:doi/10.3724%2FSP.J.1096.2011.00804&rft.externalDocID=38174304 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F93919X%2F93919X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Ffxhx%2Ffxhx.jpg |