毛细管电泳-电致化学发光联用技术检测烟草中尼古丁含量
建立了毛细管电泳-电致化学发光联用技术（CE-ECL）检测烟草浸出液中尼古丁含量的方法。考察了检测电位、检测池中Ru（bpy）32＋浓度和缓冲液pH值、CE运行缓冲液浓度和pH值、进样时间和电压,以及CE分离电压等实验条件对尼古丁测定的影响。在优化的条件下,尼古丁检测的线性范围为低浓度段：1～100μg/L（r〉0.999）、高浓度段：100～1500μg/L（r〉0.996）,检出限为0.5μg/L（S/N=3）。将本方法用于3种品牌的烟草浸出液中尼古丁含量的测量,测定值均与烟盒上尼古丁的标注值相吻合,样品的加标回收率为95%～106%。...
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| Published in | 分析化学 Vol. 41; no. 8; pp. 1274 - 1278 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
集美大学生物工程学院,厦门,361021%厦门大学近海海洋环境科学国家重点实验室,厦门,361005
2013
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2013.30024 |
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| Abstract | 建立了毛细管电泳-电致化学发光联用技术（CE-ECL）检测烟草浸出液中尼古丁含量的方法。考察了检测电位、检测池中Ru（bpy）32＋浓度和缓冲液pH值、CE运行缓冲液浓度和pH值、进样时间和电压,以及CE分离电压等实验条件对尼古丁测定的影响。在优化的条件下,尼古丁检测的线性范围为低浓度段：1～100μg/L（r〉0.999）、高浓度段：100～1500μg/L（r〉0.996）,检出限为0.5μg/L（S/N=3）。将本方法用于3种品牌的烟草浸出液中尼古丁含量的测量,测定值均与烟盒上尼古丁的标注值相吻合,样品的加标回收率为95%～106%。 |
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| AbstractList | 建立了毛细管电泳-电致化学发光联用技术(CE-ECL)检测烟草浸出液中尼古丁含量的方法.考察了检测电位、检测池中Ru(bpy)32+浓度和缓冲液pH值、CE运行缓冲液浓度和pH值、进样时间和电压,以及CE分离电压等实验条件对尼古丁测定的影响.在优化的条件下,尼古丁检测的线性范围为低浓度段:1 ～ 100 μg/L(r＞0.999)、高浓度段:100 ～ 1500 μg/L(r＞0.996),检出限为0.5 μg/L(S/N=3).将本方法用于3种品牌的烟草浸出液中尼古丁含量的测量,测定值均与烟盒上尼古丁的标注值相吻合,样品的加标回收率为95％～106％. 建立了毛细管电泳-电致化学发光联用技术（CE-ECL）检测烟草浸出液中尼古丁含量的方法。考察了检测电位、检测池中Ru（bpy）32＋浓度和缓冲液pH值、CE运行缓冲液浓度和pH值、进样时间和电压,以及CE分离电压等实验条件对尼古丁测定的影响。在优化的条件下,尼古丁检测的线性范围为低浓度段：1～100μg/L（r〉0.999）、高浓度段：100～1500μg/L（r〉0.996）,检出限为0.5μg/L（S/N=3）。将本方法用于3种品牌的烟草浸出液中尼古丁含量的测量,测定值均与烟盒上尼古丁的标注值相吻合,样品的加标回收率为95%～106%。 |
| Author | 赵利敏 陈晓梅 田晓田 黄志勇 陈曦 |
| AuthorAffiliation | 集美大学生物工程学院,厦门361021 厦门大学近海海洋环境科学国家重点实验室,厦门361005 |
| AuthorAffiliation_xml | – name: 集美大学生物工程学院,厦门,361021%厦门大学近海海洋环境科学国家重点实验室,厦门,361005 |
| Author_FL | CHEN Xiao-Mei CHEN Xi ZHAO Li-Min TIAN Xiao-Tian HUANG Zhi-Yong |
| Author_FL_xml | – sequence: 1 fullname: ZHAO Li-Min – sequence: 2 fullname: CHEN Xiao-Mei – sequence: 3 fullname: TIAN Xiao-Tian – sequence: 4 fullname: HUANG Zhi-Yong – sequence: 5 fullname: CHEN Xi |
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| DocumentTitleAlternate | Sensitive Determination of Nicotine in Tobacco by Capillary Electrophoresis Coupled with Electrochemiluminescence |
| DocumentTitle_FL | Sensitive Determination of Nicotine in Tobacco by Capillary Electrophoresis Coupled with Electrochemiluminescence |
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| Keywords | Tobacco 毛细管电泳 烟草 Capillary electrophoresis 电致化学发光 Tris (2,2'-bipyridine) ruthenium Electrochemiluminescence 尼古丁 联吡啶钌 Nicotine |
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| Notes | Capillary electrophoresis; Electrochemiluminescence; Tris（2; 2′-bipyridine）ruthenium; Nicotine; Tobacco ZHAO Li-Min ,CHEN Xiao-Mei ,TIAN Xiao-Tian ,HUANG Zhi-Yong,CHEN Xi （College of Biological Engineering,Jimei University,Xiamen 361021,China） 2（State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361005,China） 22-1125/O6 A method of capillary electrophoresis coupled with electrochemiluminescence（CE-ECL） for the determination of nicotine in tobacco samples was developed.The experimental parameters,including detection potential,concentrations of Ru（bpy）32＋ and pH values of phosphate buffer solutions（PBS） in reaction cell,concentrations and pH values of PBS for capillary electrophoresis（CE）,time and voltage of sampling,and separation voltage of CE,were optimized.Under the optimum conditions,the linear responses of ECL intensities versus nicotine concentrations were valid in the ranges of 1-100 μg/L（r0.999） and 100-1500 μg/L（r0.996）,respectively.The detection limit（S/N=3） of 0.5 μg/L was ac |
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| PublicationTitle | 分析化学 |
| PublicationTitleAlternate | Chinese Journal of Analytical Chemistry |
| PublicationTitle_FL | Chinese Journal of Analytical Chemistry |
| PublicationYear | 2013 |
| Publisher | 集美大学生物工程学院,厦门,361021%厦门大学近海海洋环境科学国家重点实验室,厦门,361005 |
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| Snippet | ... 建立了毛细管电泳-电致化学发光联用技术(CE-ECL)检测烟草浸出液中尼古丁含量的方法.考察了检测电位、检测池中Ru(bpy)32+浓度和缓冲液pH值、CE运行缓冲液浓度和pH值、进样时间和电压,以及CE分离电压等实验条件对尼古丁测定的影响.在优化的条件下,尼古丁检测的线性范围为低浓度段:1 ～ 100... |
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| SubjectTerms | 尼古丁 毛细管电泳 烟草 电致化学发光 联吡啶钌 |
| Title | 毛细管电泳-电致化学发光联用技术检测烟草中尼古丁含量 |
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