Enhanced bioethanol production by evolved Escherichia coli LGE2-H in a microbial electrolysis cell system

Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effect...

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Published inBioresources and bioprocessing Vol. 11; no. 1; p. 4
Main Authors Wang, Cong, Chang, Dongdong, Zhang, Qi, Yu, Zhisheng
Format Journal Article
LanguageEnglish
Published Singapore Springer Nature Singapore 03.01.2024
Springer Nature B.V
SpringerOpen
Subjects
Adaptive evolution
Aldehydes
Biochemical Engineering
Biofuels
Biomanufacturing Driven by Synthetic Biology
Chemistry
Chemistry and Materials Science
Clean energy
Detoxification
E coli
Electrolysis
Environmental Engineering/Biotechnology
Environmental protection
Escherichia coli
Escherichia coli LGE2-H
Ethanol
Fermentation
Industrial and Production Engineering
Levoglucosan
Lignocellulose
Lignocellulosic pyrolysate
Microbial electrolysis cell
Microorganisms
Oils & fats
Phenols
Pyrolysis
Substrates
Adaptive evolution
Microbial electrolysis cell
Lignocellulosic pyrolysate
LGE2-H
Escherichia coli LGE2-H
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Summary:Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effects on microorganisms compromise the downstream utilization and conversion of lignocellulosic pyrolysates. In this study, we constructed a microbial electrolysis cell system for bio-oil detoxification and efficient ethanol production using evolved Escherichia coli to overcome the bioethanol production and utilization challenges highlighted in previous studies. In electrically treated bio-oil media, the E. coli -H strain exhibited significantly higher levoglucosan consumption and ethanol production capacities compared with the control. In undetoxified bio-oil media containing 1.0% (w/v) levoglucosan, E. coli -H produced 0.54 g ethanol/g levoglucosan, reaching 94% of the theoretical yield. Our findings will contribute to developing a practical method for bioethanol production from lignocellulosic substrates, and provide a scientific basis and technical demonstration for its industrialized application. Graphical abstract
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ISSN:2197-4365
2197-4365
DOI:10.1186/s40643-023-00717-5