Extracellular Proteinases of Miamiensis avidus Causing Scuticociliatosis are Potential Virulence Factors
Miamiensis avidus is the causative agent of scuticociliatosis in various marine fish species. The virulence factors of the parasite have not been identified, so far. In this study, we examined M. avidus extracellular proteinases (ECPs) as potential virulence factors, using culture supernatants as an...
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| Published in | Fish Pathology Vol. 53; no. 1; pp. 1 - 9 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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Tokyo
The Japanese Society of Fish Pathology
2018
Japan Science and Technology Agency |
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| Abstract | Miamiensis avidus is the causative agent of scuticociliatosis in various marine fish species. The virulence factors of the parasite have not been identified, so far. In this study, we examined M. avidus extracellular proteinases (ECPs) as potential virulence factors, using culture supernatants as an ECPs source. We investigated the substrate specificity of ECPs using artificial peptides, and the cytotoxicity of the ECPs was examined using CHSE-214 cells. To elucidate the role of ECPs in ciliate growth, M. avidus was cultured on CHSE-214 cells in the presence of proteinase inhibitors. We detected proteinase activities from the supernatant of M. avidus. Viable CHSE-214 cells decreased significantly in number, when incubated in a medium supplemented with the culture supernatant of M. avidus. The growth of ciliates on CHSE-214 cells was delayed in the presence of PMSF (serine proteinase inhibitor) and E-64 (cysteine proteinase inhibitor). These results suggested that the culture supernatant contained ECPs showing cytotoxicity, and the proteinases facilitated nutrient uptake by the ciliates. Thus, ECPs may be responsible for virulence factors of M. avidus. |
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| AbstractList | Miamiensis avidus is the causative agent of scuticociliatosis in various marine fish species. The virulence factors of the parasite have not been identified, so far. In this study, we examined M. avidus extracellular proteinases (ECPs) as potential virulence factors, using culture supernatants as an ECPs source. We investigated the substrate specificity of ECPs using artificial peptides, and the cytotoxicity of the ECPs was examined using CHSE-214 cells. To elucidate the role of ECPs in ciliate growth, M. avidus was cultured on CHSE-214 cells in the presence of proteinase inhibitors. We detected proteinase activities from the supernatant of M. avidus. Viable CHSE-214 cells decreased significantly in number, when incubated in a medium supplemented with the culture supernatant of M. avidus. The growth of ciliates on CHSE-214 cells was delayed in the presence of PMSF (serine proteinase inhibitor) and E-64 (cysteine proteinase inhibitor). These results suggested that the culture supernatant contained ECPs showing cytotoxicity, and the proteinases facilitated nutrient uptake by the ciliates. Thus, ECPs may be responsible for virulence factors of M. avidus. |
| Author | Song, Jun-Young Narasaki, Yukie Obayashi, Yumiko Murakami, Shoko Ito, Sayami Nakayama, Kei Kitamura, Shin-Ichi |
| Author_xml | – sequence: 1 fullname: Narasaki, Yukie organization: Graduate School of Science and Engineering, Ehime University – sequence: 2 fullname: Obayashi, Yumiko organization: Center for Marine Environmental Studies (CMES), Ehime University – sequence: 3 fullname: Ito, Sayami organization: Center for Marine Environmental Studies (CMES), Ehime University – sequence: 4 fullname: Murakami, Shoko organization: Center for Marine Environmental Studies (CMES), Ehime University – sequence: 5 fullname: Song, Jun-Young organization: Pathology Division, National Fisheries Research and Development Institute – sequence: 6 fullname: Nakayama, Kei organization: Graduate School of Science and Engineering, Ehime University – sequence: 7 fullname: Kitamura, Shin-Ichi organization: Graduate School of Science and Engineering, Ehime University |
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| CitedBy_id | crossref_primary_10_1016_j_aquaculture_2021_736657 crossref_primary_10_1016_j_aquaculture_2019_734311 crossref_primary_10_1016_j_parint_2020_102093 |
| Cites_doi | 10.1016/j.ijpara.2006.08.013 10.3354/dao02017 10.1292/jvms.10-0214 10.1016/j.exppara.2010.11.005 10.3354/dao030017 10.3354/dao02116 10.3147/jsfp.35.67 10.3354/dao046047 10.1016/j.parint.2013.12.007 10.3354/dao062239 10.1354/vp.45-4-546 10.3354/dao047049 10.1007/s00436-010-2157-7 10.1111/j.1365-2761.1980.tb00400.x 10.1016/j.dci.2008.05.002 10.3147/jsfp.28.131 10.3354/ame01169 10.1016/S0166-6851(01)00438-8 10.3354/dao064159 10.3354/dao073227 10.1016/j.vetpar.2009.08.005 10.1128/IAI.01671-14 10.1016/j.fsi.2007.02.001 10.1016/j.marenvres.2015.06.011 10.1017/S0031182004004883 10.1046/j.1432-1327.1998.2540006.x 10.3354/dao062233 10.1016/j.micinf.2012.05.014 10.4319/lo.2005.50.2.0722 10.1016/j.parint.2017.10.007 10.1111/j.1365-3024.2011.01310.x 10.1111/j.1365-2761.2009.01089.x |
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| Title | Extracellular Proteinases of Miamiensis avidus Causing Scuticociliatosis are Potential Virulence Factors |
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