Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry

• Published in: Virology (New York, N.Y.) Vol. 517; pp. 9 - 15
• Main Authors: Shirato, Kazuya, Kawase, Miyuki, Matsuyama, Shutoku
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2018
• Subjects: Air-liquid interface culture; Amino Acid Sequence; animal viruses; Betacoronavirus 1; Brief Communication; cathepsin L; Cathepsin L - genetics; Cathepsins; Cell Line; Coronavirus - physiology; cultured cells; Endosomes; Entry; epithelial cells; host-pathogen relationships; Human bronchial tracheal epithelial cells; Human coronavirus; Human coronavirus 229E; Human coronavirus HKU1; Humans; pathogenesis; RNA, Messenger - genetics; RNA, Messenger - metabolism; serine; Serine Endopeptidases - physiology; serine proteinases; Spike Glycoprotein, Coronavirus; Virus Internalization; Virus Replication - physiology; DMEM; Entry; HCoV; S; Air-liquid interface culture; CatL; VHCR; Human bronchial tracheal epithelial cells; ATCC; Cam; Human coronavirus

Human coronaviruses (HCoVs) enter cells via two distinct pathways: the endosomal pathway using cathepsins to activate spike protein and the cell-surface or early endosome pathway using extracellular proteases such as transmembrane protease serine 2 (TMPRSS2). We previously reported that clinical iso...