On the Utility of Pooling Biological Samples in Microarray Experiments

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 102; no. 12; pp. 4252 - 4257
• Main Authors: Kendziorski, C., Irizarry, R. A., K.-S. Chen, Haag, J. D., Gould, M. N., Wahba, Grace
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 22.03.2005; National Acad Sciences
• Subjects: Analysis of Variance; Animals; Arithmetic mean; Biological Sciences; Biostatistics; Datasets; Diet; Experiment design; Female; Gene expression; Gene Expression Profiling - methods; Gene Expression Profiling - statistics & numerical data; Genes; Nicotinic Acids - pharmacology; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Array Sequence Analysis - statistics & numerical data; Physical Sciences; Rats; Rats, Inbred WF; Retinoid X Receptors - agonists; RNA; RNA - genetics; Statistical variance; Statistics; Tetrahydronaphthalenes - pharmacology

Over 15% of the data sets catalogued in the Gene Expression Omnibus Database involve RNA samples that have been pooled before hybridization. Pooling affects data quality and inference, but the exact effects are not yet known because pooling has not been systematically studied in the context of micro...