Specific detection and differentiation of classic goose parvovirus and novel goose parvovirus by TaqMan real-time PCR assay, coupled with host specificity

• Published in: BMC veterinary research Vol. 15; no. 1; p. 389
• Main Authors: Wan, Chunhe, Chen, Cuiteng, Cheng, Longfei, Liu, Rongchang, Shi, Shaohua, Fu, Guanghua, Chen, Hongmei, Fu, Qiuling, Huang, Yu
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01.11.2019; BioMed Central; BMC
• Subjects: Analysis; Animals; Anseriform dependoparvovirus 1; beak; breeding; Cairina moschata; China; Classic GPV; cross reaction; detection limit; Differentiation; DNA; DNA, Bacterial - genetics; DNA, Complementary - genetics; DNA, Viral - genetics; ducklings; Ducks; dwarfing; Dwarfism; epidemiological studies; Epidemiology; Escherichia coli; Fluorescence; Geese; Genes; goslings; Health aspects; Host Specificity; Morbidity; Mortality; mules; Muscovy; N-GPV; Novels; NS gene; Parvoviridae Infections - diagnosis; Parvoviridae Infections - veterinary; Parvoviridae Infections - virology; Parvovirinae - isolation & purification; pathogenesis; pathogenicity; Pekin; Polymerase Chain Reaction - methods; Poultry Diseases - diagnosis; Poultry Diseases - virology; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Specific detection; TaqMan real-time PCR assay; China; Classic GPV; Specific detection; NS gene; Differentiation; TaqMan real-time PCR assay; N-GPV

Classic goose parvovirus (cGPV) causes high mortality and morbidity in goslings and Muscovy ducklings. Novel GPV (N-GPV) causes short beak and dwarfism syndrome (SBDS) in Cherry Valley ducks, Pekin ducks and Mule ducks. Both cGPV and N-GPV have relatively strict host specificity, with obvious differ...