Regulation of alternative splicing at the single‐cell level

• Published in: Molecular systems biology Vol. 11; no. 12; pp. 845 - n/a
• Main Authors: Faigenbloom, Lior, Rubinstein, Nimrod D, Kloog, Yoel, Mayrose, Itay, Pupko, Tal, Stein, Reuven
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2015; EMBO Press; John Wiley and Sons Inc; Springer Nature
• Subjects: Alternative Splicing; Cell Differentiation; Cell fate; Conserved sequence; Differentiation (biology); EMBO17; EMBO36; Evolution, Molecular; Evolutionary conservation; Exons; Experiments; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation; Genome, Human; HEK293 Cells; Humans; inclusion level; Isoforms; Life sciences; MCF-7 Cells; RNA, Messenger - metabolism; Sample variance; single cell; Single-Cell Analysis; Splicing; splicing regulation; Stem cells; Stem Cells - cytology; single cell; inclusion level; alternative splicing; splicing regulation; evolutionary conservation

Alternative splicing is a key cellular mechanism for generating distinct isoforms, whose relative abundances regulate critical cellular processes. It is therefore essential that inclusion levels of alternative exons be tightly regulated. However, how the precision of inclusion levels among individua...