Mapping DNA damage‐dependent genetic interactions in yeast via party mating and barcode fusion genetics

• Published in: Molecular systems biology Vol. 14; no. 5; pp. e7985 - n/a
• Main Authors: Díaz‐Mejía, J Javier, Celaj, Albi, Mellor, Joseph C, Coté, Atina, Balint, Attila, Ho, Brandon, Bansal, Pritpal, Shaeri, Fatemeh, Gebbia, Marinella, Weile, Jochen, Verby, Marta, Karkhanina, Anna, Zhang, YiFan, Wong, Cassandra, Rich, Justin, Prendergast, D'Arcy, Gupta, Gaurav, Öztürk, Sedide, Durocher, Daniel, Brown, Grant W, Roth, Frederick P
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.05.2018; EMBO Press; John Wiley and Sons Inc; Springer Nature
• Subjects: Bar codes; Bleomycin; Chromosome Mapping; condition‐dependent; Damage; Deoxyribonucleic acid; DNA; DNA barcode; DNA Barcoding, Taxonomic; DNA Damage; DNA Repair; DNA sequencing; EMBO17; EMBO22; EMBO26; en masse; Epistasis, Genetic; Gene Deletion; Gene mapping; Genes; Genetic engineering; genetic interaction; Genetic Loci; Genetics; Growth conditions; High-Throughput Nucleotide Sequencing; Kinases; Mapping; Mating; Method; Methods; Methyl Methanesulfonate; Models, Theoretical; Mutants; Promoter Regions, Genetic; Protein kinase; Proteins; Recombination; Repair; Reproducibility of Results; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; sequencing; Yeast; condition‐dependent; DNA barcode; genetic interaction; sequencing; en masse

Condition‐dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State‐of‐the‐art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double‐mutant strains, does not scale readily to mu...