Biosynthetic study of conidiation-inducing factor conidiogenone: heterologous production and cyclization mechanism of a key bifunctional diterpene synthase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 83; no. 2; pp. 192 - 201
• Main Authors: Shiina, Tetsuya, Nakagawa, Kazuya, Fujisaki, Yukiko, Ozaki, Taro, Liu, Chengwei, Toyomasu, Tomonobu, Hashimoto, Masaru, Koshino, Hiroyuki, Minami, Atsushi, Kawaide, Hiroshi, Oikawa, Hideaki
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.02.2019; Oxford University Press
• Subjects: Alkyl and Aryl Transferases - genetics; Alkyl and Aryl Transferases - metabolism; Aspergillus oryzae - genetics; Biosynthesis; Carbon-13 Magnetic Resonance Spectroscopy; Cloning, Molecular; conidiogenone; Cyclization; Density Functional Theory; diterpene; Diterpenes - chemistry; Diterpenes - metabolism; DNA, Complementary - genetics; Genes, Fungal; Mass Spectrometry; Models, Molecular; Molecular Structure; NMR analysis; Penicillium - enzymology; Penicillium - genetics; Proton Magnetic Resonance Spectroscopy; Biosynthesis; diterpene; NMR analysis; conidiogenone

Conidiogenone, a diterpene with a unique structure, is known to induce the conidiation of Penicillium cyclopium. The biosynthetic pathway of (−)-conidiogenone has been fully elucidated by the heterologous expression of biosynthetic genes in Aspergillus oryzae and by in vitro enzyme assay with 13 C-l...