Application of electrospray ionization ion trap/time-of-flight mass spectrometry for chemically-synthesized small RNAs

• Published in: Journal of bioscience and bioengineering Vol. 113; no. 3; pp. 412 - 419
• Main Authors: Izumi, Yoshihiro, Takimura, Shin, Yamaguchi, Shinichi, Iida, Junko, Bamba, Takeshi, Fukusaki, Eiichiro
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.03.2012; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Chemically-synthesized small RNA; Data processing; dissociation; Electrospray ionization ion trap/time-of-flight mass spectrometry (ESI-IT/TOFMS); Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; ionization; ions; mass spectrometry; MicroRNA; MicroRNAs - analysis; MicroRNAs - chemistry; Multistep MSn analysis; RNA; RNA - analysis; RNA - chemistry; RNA modification; Sensitivity and Specificity; Sequence Analysis; Sequence Analysis, RNA - methods; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; RNA modification; MicroRNA; Multistep MSn analysis; Chemically-synthesized small RNA; Electrospray ionization ion trap/time-of-flight mass spectrometry (ESI-IT/TOFMS); Gene silencing; RNA; Micro RNA; analysis; Application; Mass spectrometry; Electrospray; Multistep MS

In this study, we have demonstrated an accurate and rapid small RNA analytical method with both sequence determination and detailed modification analysis by electrospray ionization-ion trap/time-of-flight mass spectrometry (ESI-IT/TOFMS). To develop this ideal method, we have examined the performanc...