Detection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells

• Published in: Cancer letters Vol. 403; pp. 216 - 223
• Main Authors: Li, Heming, Meng, Qing H., Noh, Hyangsoon, Batth, Izhar Singh, Somaiah, Neeta, Torres, Keila E., Xia, Xueqing, Wang, Ruoyu, Li, Shulin
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 10.09.2017; Elsevier Limited
• Subjects: Biomarkers, Tumor - blood; Bone Neoplasms - blood; Bone Neoplasms - pathology; Breast cancer; Cell Line, Tumor; Cell surface; Cell surface vimentin; Cells; Circulating tumor cells; Cryopreservation; Cryotherapy; Disease; Fluorescent Antibody Technique; Humans; Immunomagnetic Separation - methods; Laboratories; Leukocytes (mononuclear); Leukocytes, Mononuclear - chemistry; Leukocytes, Mononuclear - pathology; Lymphatic system; Metastases; Metastasis; Methods; Neoplastic Cells, Circulating - chemistry; Neoplastic Cells, Circulating - pathology; Nuclear medicine; Osteosarcoma - blood; Osteosarcoma - pathology; Peripheral blood mononuclear cells; Predictive Value of Tests; Prognosis; Reproducibility; Reproducibility of Results; Sarcoma; Therapeutic applications; Time Factors; Tumor cells; Tumor markers; Tumors; Vimentin; Vimentin - blood; Cell surface vimentin; Sarcoma; EpCAM; Cryopreservation; CSV; Peripheral blood mononuclear cells; Circulating tumor cells; PBMCs; EMT; CTCs

Circulating tumor cells (CTCs) enter the vasculature or lymphatic system after shedding from the primary tumor. CTCs may serve as “seed” cells for tumor metastasis. The utility of CTCs in clinical applications for sarcoma is not fully investigated, partly owing to the necessity for fresh blood sampl...