Determination of 1-Hydroxypyrene in Human Urine by Acid Hydrolysis Coupled to Solid-Phase Microextraction and Semi-microcolumn Liquid Chromatography

• Published in: Analytical Sciences Vol. 23; no. 10; pp. 1221 - 1225
• Main Author: CHEN, Hong-Wen
• Format: Journal Article
• Language: English
• Published: Japan The Japan Society for Analytical Chemistry 01.10.2007; Japan Science and Technology Agency
• Subjects: Acids - chemistry; Chromatography, Liquid - methods; Humans; Hydrogen-Ion Concentration; Hydrolysis; Osmolar Concentration; Pyrenes - analysis; Solid Phase Extraction - methods; Time Factors; Urine - chemistry
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.23.1221

This study developed an acid hydrolysis coupled to a solid-phase microextraction method employing a semi-microcolumn liquid chromatography system, instead of enzyme hydrolysis with solid-phase extraction for the pretreatment of human urine samples, to detect urinary 1-hydroxypyrene (1-OHP). The complete separation and detection of urinary 1-hydroxyprene was performed using a high-performance liquid-chromatography fluorescence detection system with an analytical C18 semi-microcolumn, 60% (v/v) aqueous acetonitrile elution, and a λex/em = 348/388 nm pair detection wavelength. Calibration graphs were linear with very good correlation coefficients (r = 0.9997), and the detection limit was 1.0 ng/L. These important parameters for acid hydrolysis and solid-phase microextraction were investigated. The total recovery was above 83% in acid hydrolysis with solid-phase microextraction. The proposed method provided a relatively simple, convenient, and practical procedure to determine the level of urinary 1-hydroxypyrene in biological samples, and was successfully applied to detect the urine of students.