High V-PPase activity is beneficial under high salt loads, but detrimental without salinity

• Published in: The New phytologist Vol. 219; no. 4; pp. 1421 - 1432
• Main Authors: Graus, Dorothea, Konrad, Kai R., Bemm, Felix, Patir Nebioglu, Meliha Görkem, Lorey, Christian, Duscha, Kerstin, Güthoff, Tilman, Herrmann, Johannes, Ferjani, Ali, Cuin, Tracey Ann, Roelfsema, M. Rob G., Schumacher, Karin, Neuhaus, H. Ekkehard, Marten, Irene, Hedrich, Rainer
• Format: Journal Article
• Language: English
• Published: England New Phytologist Trust 01.09.2018; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Subjects: Acidification; Adenosine triphosphatase; Capacity; Cell death; Cell Death - drug effects; Cell Membrane - drug effects; Cell Membrane - metabolism; cell viability; Depolarization; Diphosphates - metabolism; electrodes; enzyme activity; functional properties; gene overexpression; Growth conditions; H-transporting ATP synthase; Hydrogen-Ion Concentration; hydrolysis; Hyperactivity; image analysis; Imaging techniques; Inorganic Pyrophosphatase - metabolism; Isoenzymes - metabolism; Leaves; Membrane potential; Membrane Potentials - drug effects; Mesophyll Cells - drug effects; Mesophyll Cells - enzymology; Necrosis; Negative feedback; Nicotiana - drug effects; Nicotiana - enzymology; Nicotiana benthamiana; pH effects; Photosynthesis; Plant Epidermis - cytology; Plant Epidermis - drug effects; plasma membrane; plasma membrane voltage; proton pump; proton pump currents; Proton Pumps - metabolism; proton-motive force; Protonmotive force; Protons; Pumping; Pyrophosphatase; Salinity; Salinity effects; Salinity tolerance; salt; salt stress; Salt tolerance; sodium; Sodium Chloride - pharmacology; Solute transport; Solutes; Stress, Physiological - drug effects; Transgenic plants; vacuolar pH; Vacuolar Proton-Translocating ATPases - metabolism; vacuolar proton‐ATPase (V‐ATPase); vacuolar proton‐pyrophosphatase (V‐PPase); vacuoles; Vacuoles - enzymology; salt; vacuolar proton-pyrophosphatase (V-PPase); cell death; vacuolar pH; vacuolar proton-ATPase (V-ATPase); proton pump currents; plasma membrane voltage

The membrane-bound proton-pumping pyrophosphatase (V-PPase), together with the V-type H+-ATPase, generates the proton motive force that drives vacuolar membrane solute transport. Transgenic plants constitutively overexpressing V-PPases were shown to have improved salinity tolerance, but the relative...