Quantitative detection of T315I mutations of BCR::ABL1 using digital droplet polymerase chain reaction

• Published in: Hematology, Transfusion and Cell Therapy Vol. 46; no. Suppl 6; pp. S79 - S85
• Main Authors: Mu, Huijun, Zou, Jian, Zhang, Haiping
• Format: Journal Article
• Language: English
• Published: Brazil Elsevier España, S.L.U 01.12.2024; Sociedade Brasileira de Hematologia e Hemoterapia; Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular (ABHH); Elsevier
• Subjects: BCR::ABL1 gene; Digital droplet polymerase chain reaction; MEDICINE, GENERAL & INTERNAL; Original; Philadelphia chromosome; T315I mutation; T315I mutation; Digital droplet polymerase chain reaction; BCR::ABL1 gene; Philadelphia chromosome

T315I mutations of the BCR::ABL1 gene lead to resistance to tyrosine kinase inhibitors (TKIs). This study evaluated the performance of digital droplet polymerase chain reaction (ddPCR) in quantifying T315I mutations and their frequency in Philadelphia chromosome (Ph) positive hematological patients....