My various thoughts on actin
An enormous amount of research has been performed to characterize actin dynamics. Structural biology investigations have determined the localization of main chains and their changes coupled with G (Globular)-F (Filamentous) transformation of actin, whereas local thermal fluctuations that may be caus...
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| Published in | Biophysics and Physicobiology Vol. 15; pp. 151 - 158 |
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| Format | Journal Article |
| Language | English |
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Japan
The Biophysical Society of Japan
01.01.2018
The Biophysical Society of Japan (BSJ) |
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| Abstract | An enormous amount of research has been performed to characterize actin dynamics. Structural biology investigations have determined the localization of main chains and their changes coupled with G (Globular)-F (Filamentous) transformation of actin, whereas local thermal fluctuations that may be caused by free rotations of the tips of side chains are not yet fully investigated. This paper argues if the entropy change of actin accompanied by the G-F transformation is simply attributable to the changes in hydration. It took almost 10 years to understand that the actin filament is semi-flexible. This flexibility was visually confirmed as the development of optical microscope techniques, and the direct observation of actin severing events in the presence of actin binding proteins became possible. Finally, I expect the deep understanding of actin dynamics will lead to the elucidation of self-assembly mechanisms of the living creature. |
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| AbstractList | An enormous amount of research has been performed to characterize actin dynamics. Structural biology investigations have determined the localization of main chains and their changes coupled with G (Globular)-F (Filamentous) transformation of actin, whereas local thermal fluctuations that may be caused by free rotations of the tips of side chains are not yet fully investigated. This paper argues if the entropy change of actin accompanied by the G-F transformation is simply attributable to the changes in hydration. It took almost 10 years to understand that the actin filament is semi-flexible. This flexibility was visually confirmed as the development of optical microscope techniques, and the direct observation of actin severing events in the presence of actin binding proteins became possible. Finally, I expect the deep understanding of actin dynamics will lead to the elucidation of self-assembly mechanisms of the living creature. |
| Author | Oosawa, Fumio |
| AuthorAffiliation | 3 Department of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan 1 Department of Physics, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan 2 Institute of Molecular Biology, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30105175$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_bpj_2020_06_019 crossref_primary_10_2142_biophysico_bppb_v19_0025 |
| Cites_doi | 10.1038/ncb3142 10.1021/bi00553a021 10.1093/oxfordjournals.jbchem.a128158 10.1126/science.152.3730.1756 10.1038/nature07685 10.1016/0022-2836(80)90311-3 10.1002/cm.21384 10.1016/S0006-3495(98)74001-9 10.1002/pol.1960.1204414304 10.1002/cm.21421 10.1093/oxfordjournals.jbchem.a128167 10.1002/cm.21416 10.1021/bi00369a034 10.1038/173973a0 10.1016/0005-2728(69)90209-6 10.1083/jcb.87.3.569 10.1143/JPSJ.29.751 10.1002/cm.21391 10.1021/acs.jpcb.6b02584 10.1002/cm.21417 10.1016/0304-4165(66)90402-8 |
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| Keywords | actin binding proteins flexibility of actin filament actin thermodynamics entropy changes in G-F transformation hydration of actin |
| Language | English |
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| Title | My various thoughts on actin |
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