DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH
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Published in | Journal of Bacteriology Vol. 170; no. 7; pp. 3150 - 3157 |
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01.07.1988
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AbstractList | Nucleotide sequences of the cysK regions of Salmonella typhimurium and Escherichia coli have been determined. A total of 3,812 and 2,595 nucleotides were sequenced from S. typhimurium and E. coli, respectively. Open reading frames of 323 codons were found in both species and were identified as those of cysK by comparison of deduced amino acid sequences with amino- and carboxyl-terminal amino acid analyses of the S. typhimurium cysK gene product O-acetylserine (thiol)-lyase A. The two cysK DNA sequences were 85% identical, and the deduced amino acid sequences were 96% identical. The major transcription initiation sites for cysK were found to be virtually identical in the two organisms, by using primer extension and S1 nuclease protection techniques. The -35 region corresponding to the major transcription start site was TTCCCC in S. typhimurium and TTCCGC in E. coli. The deviation of these sequences from the consensus sequence TTGACA may reflect the fact that cysK is subject to positive control and requires the cysB regulatory protein for expression. Sequences downstream of cysK were found to include ptsH and a portion of ptsI, thus establishing the exact relationship of cysK with these two genes. A 290-codon open reading frame, which may represent the cysZ gene, was identified upstream of cysK. Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JB About JB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0021-9193 Online ISSN: 1098-5530 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JB .asm.org, visit: JB Nucleotide sequences of the cysK regions of Salmonella typhimurium and Escherichia coli have been determined. A total of 3,812 and 2,595 nucleotides were sequenced from S. typhimurium and E. coli, respectively. Open reading frames of 323 codons were found in both species and were identified as those of cysK by comparison of deduced amino acid sequences with amino- and carboxyl-terminal amino acid analyses of the S. typhimurium cysK gene product O-acetylserine (thiol)-lyase A. The two cysK DNA sequences were 85% identical, and the deduced amino acid sequences were 96% identical. The major transcription initiation sites for cysK were found to be virtually identical in the two organisms, by using primer extension and S1 nuclease protection techniques. The -35 region corresponding to the major transcription start site was TTCCCC in S. typhimurium and TTCCGC in E. coli. The deviation of these sequences from the consensus sequence TTGACA may reflect the fact that cysK is subject to positive control and requires the cysB regulatory protein for expression. Sequences downstream of cysK were found to include ptsH and a portion of ptsI, thus establishing the exact relationship of cysK with these two genes. A 290-codon open reading frame, which may represent the cysZ gene, was identified upstream of cysK.Nucleotide sequences of the cysK regions of Salmonella typhimurium and Escherichia coli have been determined. A total of 3,812 and 2,595 nucleotides were sequenced from S. typhimurium and E. coli, respectively. Open reading frames of 323 codons were found in both species and were identified as those of cysK by comparison of deduced amino acid sequences with amino- and carboxyl-terminal amino acid analyses of the S. typhimurium cysK gene product O-acetylserine (thiol)-lyase A. The two cysK DNA sequences were 85% identical, and the deduced amino acid sequences were 96% identical. The major transcription initiation sites for cysK were found to be virtually identical in the two organisms, by using primer extension and S1 nuclease protection techniques. The -35 region corresponding to the major transcription start site was TTCCCC in S. typhimurium and TTCCGC in E. coli. The deviation of these sequences from the consensus sequence TTGACA may reflect the fact that cysK is subject to positive control and requires the cysB regulatory protein for expression. Sequences downstream of cysK were found to include ptsH and a portion of ptsI, thus establishing the exact relationship of cysK with these two genes. A 290-codon open reading frame, which may represent the cysZ gene, was identified upstream of cysK. Nucleotide sequences of the cysK regions of Salmonella typhimurium and Escherichia coli have been determined. A total of 3,812 and 2,595 nucleotides were sequenced from S. typhimurium and E. coli , respectively. Open reading frames of 323 codons were found in both species and were identified as those of cysK by comparison of deduced amino acid sequences with amino- and carboxyl-terminal amino acid analyses of the S. typhimurium cysK gene product O-acetylserine (thiol)-lyase A. |
Author | N M Kredich C R Byrne R S Monroe K A Ward |
AuthorAffiliation | Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia |
AuthorAffiliation_xml | – name: Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia |
Author_xml | – sequence: 1 givenname: C R surname: Byrne fullname: Byrne, C R organization: Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia – sequence: 2 givenname: R S surname: Monroe fullname: Monroe, R S organization: Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia – sequence: 3 givenname: K A surname: Ward fullname: Ward, K A organization: Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia – sequence: 4 givenname: N M surname: Kredich fullname: Kredich, N M organization: Division of Animal Production, Commonwealth Scientific and Industrial Research Organisation, Blacktown, New South Wales, Australia |
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Keywords | Cysteine Nucleotide sequence Enzyme Escherichia coli Gene organization Biosynthesis Gene expression Salmonella typhimurium Regulation(control) Gene Bacteria Escherichieae Enterobacteriaceae D-Acetylserine (thiol)-lyase |
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Mendeley... Nucleotide sequences of the cysK regions of Salmonella typhimurium and Escherichia coli have been determined. A total of 3,812 and 2,595 nucleotides were... |
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SubjectTerms | Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Codon - genetics Cysteine Synthase - genetics Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic Linkage Genetics Lyases - genetics Microbiology Molecular Sequence Data Plasmids Promoter Regions, Genetic Salmonella typhimurium Salmonella typhimurium - enzymology Salmonella typhimurium - genetics Transcription, Genetic |
Title | DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH |
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