Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use

• Published in: Journal of biomedical science Vol. 29; no. 1; pp. 9 - 17
• Main Authors: Tu, I-Fan, Lin, Tzu-Lung, Yang, Feng-Ling, Lee, I-Ming, Tu, Wei-Lin, Liao, Jiahn-Haur, Ko, Tzu-Ping, Wu, Wen-Jin, Jan, Jia-Tsrong, Ho, Meng-Ru, Chou, Ching-Yi, Wang, Andrew H.-J., Wu, Chung-Yi, Wang, Jin-Town, Huang, Kai-Fa, Wu, Shih-Hsiung
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.02.2022; BioMed Central; BMC
• Subjects: Abscesses; Absorption; Acetates; Acetylation; Animals; Antigenic determinants; Antigens; Bacteria; Bacterial Capsules - genetics; Bacterial pneumonia; Bacteriophages - genetics; Binding sites; Biodegradation; Capsular polysaccharides; Carbohydrates; Chemical compounds; Crystal structure; Crystals; Depolymerization; Enzymes; Experiments; Fragments; Health aspects; Humans; Immune response; Infections; Infectivity; K1 capsular polysaccharide; Kinetics; Klebsiella; Klebsiella Infections; Klebsiella pneumoniae; Klebsiella pnuemoniae; Liver; Lyases; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; Meningitis; Mice; Mortality; Mutagenesis; NMR; NMR spectroscopy; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Oligosaccharides; Phages; Pharmacology; Phospholipase A; Pneumonia; Polysaccharide lyase; Polysaccharides; Proteins; Pyruvic acid; Pyruvylation; Scientific imaging; Site-directed mutagenesis; Structure; Tailspike protein; Taiwan; Vaccines; Taiwan; Pyruvylation; β-Helix; K1 capsular polysaccharide; Tailspike protein; Acetylation; Polysaccharide lyase; Klebsiella pnuemoniae
• ISSN: 1423-0127; 1021-7770; 1423-0127
• DOI: 10.1186/s12929-022-00792-4

K1 capsular polysaccharide (CPS)-associated Klebsiella pneumoniae is the primary cause of pyogenic liver abscesses (PLA) in Asia. Patients with PLA often have serious complications, ultimately leading to a mortality of ~ 5%. This K1 CPS has been reported as a promising target for development of glyc...