Comparison of sampling methods in assessing the microbiome from patients with ulcerative colitis

• Published in: BMC gastroenterology Vol. 21; no. 1; pp. 396 - 13
• Main Authors: Kim, Dan, Jung, Jun-Young, Oh, Hyun-Seok, Jee, Sam-Ryong, Park, Sung Jae, Lee, Sang-Heon, Yoon, Jun-Sik, Yu, Seung Jung, Yoon, In-Cheol, Lee, Hong Sub
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 22.10.2021; BioMed Central; BMC
• Subjects: Adult; Age; Aged; Biopsy; Blood tests; Colitis, Ulcerative; Colon; Colon lavage; Colonoscopy; Diagnosis; Discriminant analysis; Disease; Dysbacteriosis; Dysbiosis; Endoscopy; Female; Gastroenterology; Gastrointestinal Microbiome; Health aspects; Humans; Inflammatory bowel disease; Lavage; Male; Medical screening; Microbiome; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Middle Aged; Mucosa; Next-generation sequencing; Polyethylene glycol; RNA, Ribosomal, 16S - genetics; rRNA 16S; Teaching hospitals; Ulcerative colitis; Variance analysis; Veganism; South Korea; Japan; Inflammatory bowel disease; Colonoscopy; Colon lavage; Microbiome; Ulcerative colitis

Dysbiosis of ulcerative colitis (UC) has been frequently investigated using readily accessible stool samples. However, stool samples might insufficiently represent the mucosa-associated microbiome status. We hypothesized that luminal contents including loosely adherent luminal bacteria after bowel p...