Fungal Light-Oxygen-Voltage Domains for Optogenetic Control of Gene Expression and Flocculation in Yeast

• Published in: mBio Vol. 9; no. 4
• Main Authors: Salinas, Francisco, Rojas, Vicente, Delgado, Verónica, López, Javiera, Agosin, Eduardo, Larrondo, Luis F
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 31.07.2018
• Subjects: Cell Adhesion; filamentous fungi; flocculation; Gene Expression Regulation, Fungal - radiation effects; heterologous gene expression; LOV domain; optogenetics; Optogenetics - methods; Photic Stimulation; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae - radiation effects; yeasts; filamentous fungi; yeasts; LOV domain; flocculation; heterologous gene expression; optogenetics

Optogenetic switches permit accurate control of gene expression upon light stimulation. These synthetic switches have become a powerful tool for gene regulation, allowing modulation of customized phenotypes, overcoming the obstacles of chemical inducers, and replacing their use by an inexpensive res...