Responses of Fibroblasts to Anchorage of Dorsal Extracellular Matrix Receptors

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 101; no. 52; pp. 18024 - 18029
• Main Authors: Beningo, Karen A, Dembo, Micah, Wang, Yu-li
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28.12.2004; National Acad Sciences
• Subjects: 3T3 cells; Acrylic Resins - chemistry; Actins - metabolism; Animals; Biological Sciences; Calcium; Calcium - metabolism; Cell Adhesion; Cell Differentiation; Cell Line; Cell Membrane - metabolism; Cell Movement; Cell Proliferation; Cells, Cultured; Cellular biology; Collagen - chemistry; Cultured cells; Cytoskeleton - ultrastructure; Digital rights management; Epithelial cells; Extracellular Matrix - metabolism; Fibrinogen - chemistry; Fibroblasts; Fibroblasts - cytology; Fibronectins - chemistry; Focal adhesions; Gene expression; Gene Expression Regulation; Image Processing, Computer-Assisted; Mice; Microscopy, Fluorescence; Models, Biological; NIH 3T3 Cells; Pseudopodia; Pseudopodia - metabolism; Receptors; Signal Transduction; Stress, Mechanical; Transfection

Fibroblasts in 2D cultures differ dramatically in behavior from those in the 3D environment of a multicellular organism. However, the basis of this disparity is unknown. A key difference is the spatial arrangement of anchored extracellular matrix (ECM) receptors to the ventral surface in 2D cultures...