TRiC/CCT Complex, a Binding Partner of NS1 Protein, Supports the Replication of Zika Virus in Both Mammalians and Mosquitoes

• Published in: Viruses Vol. 12; no. 5; p. 519
• Main Authors: Wang, Yuchen, Uraki, Ryuta, Hwang, Jesse, Fikrig, Erol
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.05.2020; MDPI
• Subjects: Aedes - genetics; Aedes - metabolism; Aedes - virology; Aedes aegypti mosquito; Animals; Arthropods; Binding proteins; Chaperonin Containing TCP-1 - genetics; Chaperonin Containing TCP-1 - metabolism; Cloning; Cytotoxicity; Female; Host-Pathogen Interactions; Humans; Immunoglobulins; Infections; Insect Proteins - genetics; Insect Proteins - metabolism; Laboratory animals; Mammalian cells; Mammals; Mass spectrometry; Mass spectroscopy; Medical research; Microencephaly; Microscopy; Mosquito Vectors - genetics; Mosquito Vectors - metabolism; Mosquito Vectors - virology; Mosquitoes; NS1 protein; Observations; Pathogenesis; Physiological aspects; Plasmids; Protein Binding; Proteins; Replication; Scientific imaging; Therapeutic applications; TRiC/CCT complex; Vaccines; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; viral replication; Virus Replication; Zika virus; Zika Virus - genetics; Zika Virus - physiology; Zika Virus Infection - genetics; Zika Virus Infection - metabolism; Zika Virus Infection - virology; Japan; United States > US; viral replication; Aedes aegypti mosquito; Zika virus; TRiC/CCT complex; NS1 protein
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v12050519

Mosquito-borne Zika virus (ZIKV) can cause congenital microcephaly and Guillain–Barré syndrome, among other symptoms. Specific treatments and vaccines for ZIKV are not currently available. To further understand the host factors that support ZIKV replication, we used mass spectrometry to characterize mammalian proteins that associate with the ZIKV NS1 protein and identified the TRiC/CCT complex as an interacting partner. Furthermore, the suppression of CCT2, one of the critical components of the TRiC/CCT complex, inhibited ZIKV replication in both mammalian cells and mosquitoes. These results highlight an important role for the TRiC/CCT complex in ZIKV infection, suggesting that the TRiC/CCT complex may be a promising therapeutic target.