Cross-protection against influenza virus infection by intranasal administration of M1-based vaccine with chitosan as an adjuvant

• Published in: Vaccine Vol. 28; no. 48; pp. 7690 - 7698
• Main Authors: Sui, Zhiwei, Chen, Quanjiao, Fang, Fang, Zheng, Mei, Chen, Ze
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 10.11.2010; Elsevier; Elsevier Limited
• Subjects: Adjuvants, Immunologic - administration & dosage; Administration, Intranasal; Allergy and Immunology; Animals; Antibodies, Viral - blood; Antibody Formation; Applied microbiology; Avian flu; Biological and medical sciences; Chickens; Chitosan; Chitosan - immunology; Cross Protection; Deoxyribonucleic acid; DNA; E coli; Escherichia coli; Escherichia coli - metabolism; Female; Fundamental and applied biological sciences. Psychology; Immunity, Cellular; Immunization; Immunization, Passive; Infections; Influenza; Influenza A virus; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H5N1 Subtype - immunology; Influenza A Virus, H9N2 Subtype - immunology; Influenza Vaccines - administration & dosage; Influenza Vaccines - immunology; Influenza virus; Interferon-gamma - immunology; Intranasal administration; M1 protein; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Mucosal adjuvant; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - prevention & control; Plasmids; Proteins; Studies; Survival; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Subunit - immunology; Viral Matrix Proteins - immunology; Virology; Viruses; Mucosal adjuvant; Intranasal administration; Chitosan; M1 protein; Influenza; Mucosa; Cross protection; Orthomyxoviridae; Polymer; Vaccine; Influenzavirus; Protein; Infection; Virus; Immunological adjuvant; Viral disease

The antigenic variation of influenza virus represents a major health problem, thus continuous efforts have been made to develop broad-spectrum vaccines against influenza virus. Matrix protein 1 (M1) protein is highly conserved in all influenza A strains. In this study, M1 protein was efficiently exp...