Determination of bixin and norbixin in meat using liquid chromatography and photodiode array detection

• Published in: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Vol. 26; no. 1; pp. 17 - 24
• Main Authors: Noppe, H, Abuin Martinez, S, Verheyden, K, Loco, J. Van, Companyo Beltran, R, Brabander, H.F. De
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Group 01.01.2009; Taylor & Francis
• Subjects: Animals; annatto; annatto dye; Biological and medical sciences; Bixaceae; bixin; Carotenoids - analysis; chemical structure; Chromatography, Liquid - methods; dyes; Food Analysis - methods; food coloring; Food Coloring Agents - analysis; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; liquid chromatography; mass spectrometry; Mass Spectrometry - methods; meat; Meat - analysis; Meat and meat product industries; meat composition; meat products; Methods of analysis, processing and quality control, regulation, standards; norbixin; photodiode array; Plant Extracts - analysis; quality control; quantitative analysis; Reproducibility of Results; reversed-phase high performance liquid chromatography; validity; annatto; Analysis method; Bixin; norbixin; Meat product; Liquid chromatography; Control method; photodiode array; Photodiode; Detection; Meat
• ISSN: 0265-203X; 1944-0049; 1464-5122; 1944-0057
• DOI: 10.1080/02652030802322564

The development of an analytical method that enables routine analysis of annatto dye, specifically bixin and norbixin, in meat tissue is described. Liquid-solid extraction was carried out using acetonitrile. Analysis was by HPLC with photodiode array detection using two fixed wavelengths (458 and 486 nm). The possibilities of ion trap mass spectrometry (MS) were also assessed. Method performance characteristics, according to Commission Decision 2002/657/EC, were determined, with recoveries between 99 and 102% and calibration curves being linear in the 0.5-10 mg kg-1 range. The limit of quantification was 0.5 mg kg-1.