Super-resolved live-cell imaging using random illumination microscopy

• Published in: Cell reports methods Vol. 1; no. 1; p. 100009
• Main Authors: Mangeat, Thomas, Labouesse, Simon, Allain, Marc, Negash, Awoke, Martin, Emmanuel, Guénolé, Aude, Poincloux, Renaud, Estibal, Claire, Bouissou, Anaïs, Cantaloube, Sylvain, Vega, Elodie, Li, Tong, Rouvière, Christian, Allart, Sophie, Keller, Debora, Debarnot, Valentin, Wang, Xia Bo, Michaux, Grégoire, Pinot, Mathieu, Le Borgne, Roland, Tournier, Sylvie, Suzanne, Magali, Idier, Jérome, Sentenac, Anne
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.05.2021; Cell Press Elsevier; Elsevier
• Subjects: aberration; Animals; Biochemistry, Molecular Biology; computational imaging; Drosophila; Engineering Sciences; fluorescence; Image Processing, Computer-Assisted; Life Sciences; Lighting; live imaging; microscopy; Microscopy, Fluorescence; Optics; Photonic; Signal and Image processing; speckle; super-resolution; variance; speckle; live imaging; fluorescence; variance; super-resolution; aberration; microscopy; computational imaging

Current super-resolution microscopy (SRM) methods suffer from an intrinsic complexity that might curtail their routine use in cell biology. We describe here random illumination microscopy (RIM) for live-cell imaging at super-resolutions matching that of 3D structured illumination microscopy, in a ro...