Cytotoxic Activity of Coagulase-Negative Staphylococci in Bovine Mastitis

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Published inInfection and Immunity Vol. 68; no. 3; pp. 1102 - 1108
Main Authors SONGLIN ZHANG, MADDOX, C. W
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.03.2000
Subjects
34-36-kDa protein
Animals
Bacterial Toxins - toxicity
Bacteriology
Biological and medical sciences
Cattle
CHO Cells
Coagulase - analysis
Cricetinae
Female
Fundamental and applied biological sciences. Psychology
Humans
Mastitis, Bovine - microbiology
Metalloendopeptidases - toxicity
Metalloproteinase
Microbiology
Molecular and Cellular Pathogenesis
Molecular Weight
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Staphylococcus
Staphylococcus - pathogenicity
Staphylococcus chromogenes
Virulence
Mastitis
Bovine
Enzyme
Host agent relation
Cytotoxicity
Metalloendopeptidases
Biological activity
Protein
Staphylococcus
Characterization
Mammary gland diseases
Peptidases
Toxin
Vertebrata
Mammalia
Bacteria
Hydrolases
Micrococcales
Micrococcaceae
Artiodactyla
Clinical isolate
Ungulata
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Abstract Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue IAI About IAI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy Connect to IAI IAI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0019-9567 Online ISSN: 1098-5522 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to IAI .asm.org, visit: IAI       
AbstractList Secreted toxins play important roles in the pathogenesis of bacterial infections. In this study, we examined the presence of secreted cytotoxic factors of coagulase-negative Staphylococci (CoNS) from bovine clinical and subclinical mastitis. A 34- to 36-kDa protein with cell-rounding cytotoxic activity was found in many CoNS strains, especially in Staphylococcus chromogenes strains. The protein caused cell detachment and cell rounding in several cell lines, including HEp-2, Int 407, CHO-K1, and Y-1 cells. Native protein recovered from nondenatured polyacrylamide gel electrophoresis showed both cytotoxic activity and casein hydrolysis activity. The purified protein had a pH optimal at 7.2 to 7.5 and a pI of 5.1 and was heat labile. The proteolytic activity could be inhibited by zinc and metal specific inhibitors such as 1,10-phenanthroline and EDTA, indicating that it is a metalloprotease. Protein mass analysis and peptide sequencing indicated that the protein is a novel metalloprotease. Different bacterial strains expressed variable levels of 34- to 36-kDa protease, which may provide an indication of strain virulence.
Secreted toxins play important roles in the pathogenesis of bacterial infections. In this study, we examined the presence of secreted cytotoxic factors of coagulase-negative staphylococci (CoNS) from bovine clinical and subclinical mastitis. A 34- to 36-kDa protein with cell-rounding cytotoxic activity was found in many CoNS strains, especially in Staphylococcus chromogenes strains. The protein caused cell detachment and cell rounding in several cell lines, including HEp-2, Int 407, CHO-K1, and Y-1 cells. Native protein recovered from nondenatured polyacrylamide gel electrophoresis showed both cytotoxic activity and casein hydrolysis activity. The purified protein had a pH optimal at 7.2 to 7.5 and a pI of 5.1 and was heat labile. The proteolytic activity could be inhibited by zinc and metal specific inhibitors such as 1,10-phenanthroline and EDTA, indicating that it is a metalloprotease. Protein mass analysis and peptide sequencing indicated that the protein is a novel metalloprotease. Different bacterial strains expressed variable levels of 34- to 36-kDa protease, which may provide an indication of strain virulence.
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ABSTRACT Secreted toxins play important roles in the pathogenesis of bacterial infections. In this study, we examined the presence of secreted cytotoxic factors of coagulase-negative staphylococci (CoNS) from bovine clinical and subclinical mastitis. A 34- to 36-kDa protein with cell-rounding cytotoxic activity was found in many CoNS strains, especially in Staphylococcus chromogenes strains. The protein caused cell detachment and cell rounding in several cell lines, including HEp-2, Int 407, CHO-K1, and Y-1 cells. Native protein recovered from nondenatured polyacrylamide gel electrophoresis showed both cytotoxic activity and casein hydrolysis activity. The purified protein had a pH optimal at 7.2 to 7.5 and a pI of 5.1 and was heat labile. The proteolytic activity could be inhibited by zinc and metal specific inhibitors such as 1,10-phenanthroline and EDTA, indicating that it is a metalloprotease. Protein mass analysis and peptide sequencing indicated that the protein is a novel metalloprotease. Different bacterial strains expressed variable levels of 34- to 36-kDa protease, which may provide an indication of strain virulence.
Author Carol W. Maddox
Songlin Zhang
AuthorAffiliation Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802
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Issue 3
Keywords Mastitis
Bovine
Enzyme
Host agent relation
Cytotoxicity
Metalloendopeptidases
Biological activity
Protein
Staphylococcus
Characterization
Mammary gland diseases
Peptidases
Toxin
Vertebrata
Mammalia
Bacteria
Hydrolases
Micrococcales
Micrococcaceae
Artiodactyla
Clinical isolate
Ungulata
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Corresponding author. Mailing address: Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-0838. Fax: (814) 865-3907. E-mail: cwm5@psu.edu.
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Snippet Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit...
Secreted toxins play important roles in the pathogenesis of bacterial infections. In this study, we examined the presence of secreted cytotoxic factors of...
ABSTRACT Secreted toxins play important roles in the pathogenesis of bacterial infections. In this study, we examined the presence of secreted cytotoxic...
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StartPage 1102
SubjectTerms 34-36-kDa protein
Animals
Bacterial Toxins - toxicity
Bacteriology
Biological and medical sciences
Cattle
CHO Cells
Coagulase - analysis
Cricetinae
Female
Fundamental and applied biological sciences. Psychology
Humans
Mastitis, Bovine - microbiology
Metalloendopeptidases - toxicity
Metalloproteinase
Microbiology
Molecular and Cellular Pathogenesis
Molecular Weight
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Staphylococcus
Staphylococcus - pathogenicity
Staphylococcus chromogenes
Virulence
Title Cytotoxic Activity of Coagulase-Negative Staphylococci in Bovine Mastitis
URI http://iai.asm.org/content/68/3/1102.abstract
https://www.ncbi.nlm.nih.gov/pubmed/10678913
https://search.proquest.com/docview/17493428
https://pubmed.ncbi.nlm.nih.gov/PMC97254
Volume 68
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