Detection of chicken chapparvovirus 2 in chickens with hemorrhagic hepatitis in Japan

• Published in: Journal of Veterinary Medical Science Vol. 86; no. 4; pp. 396 - 399
• Main Authors: FUJINO, Kan, HORIE, Masayuki, AIHARA, Naoyuki, KAMIIE, Junichi, TAHARAGUCHI, Satoshi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2024; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Anemia; Animals; Chicken anemia virus; chicken chapparvovirus 2; Chickens; Diarrhea; Feces; Hemorrhage; Hemorrhage - veterinary; hemorrhagic hepatitis; Hepatitis; Hepatitis A - veterinary; Hepatocytes; Inclusion bodies; Japan - epidemiology; Necropsy; Next-generation sequencing; Poultry Diseases; Sequence analysis; Virology; Japan; chicken chapparvovirus 2; chicken anemia virus; hemorrhagic hepatitis
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.23-0415

Chicken chaphamaparvovirus causes diarrheal symptoms and can be detected in fecal samples. This study reports the detection of chicken chapparvovirus 2 in debilitated chickens with hemorrhagic hepatitis at a broiler farm in Japan. After euthanasia and necropsy, liver hemorrhage was observed. Nuclear inclusion bodies in the hepatocytes were identified using histological analysis. High-throughput sequencing analysis using RNA from livers of three affected chickens revealed infection by chicken chapparvovirus 2 and chicken anemia virus. Polymerase chain reaction analysis showed that all three chickens were positive for chicken chapparvovirus 2, and only one was positive for both chicken chapparvovirus 2 and chicken anemia virus. In conclusion, chicken chapparvovirus 2 causes infection in chickens in Japan and might be involved in hemorrhagic hepatitis.