A mechanistic kinetic description of lactate dehydrogenase elucidating cancer diagnosis and inhibitor evaluation

• Published in: Journal of enzyme inhibition and medicinal chemistry Vol. 32; no. 1; pp. 564 - 571
• Main Authors: Tang, Peifeng, Xu, Jianlin, Oliveira, Christopher L., Li, Zheng Jian, Liu, Shijie
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2017; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Cancer; Cancer diagnosis; Dehydrogenases; Diagnosis; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Enzymes; Glycolysis; Humans; inhibitor evaluation; kinetic model; Kinetics; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - antagonists & inhibitors; L-Lactate Dehydrogenase - metabolism; lactate dehydrogenase; Lactic acid; Medical diagnosis; Molecular Dynamics Simulation; Neoplasms - diagnosis; Neoplasms - enzymology; Neoplasms - metabolism; oligomeric enzyme; Temperature; Temperature effects; lactate dehydrogenase; inhibitor evaluation; oligomeric enzyme; Cancer diagnosis; kinetic model

As a key enzyme for glycolysis, lactate dehydrogenase (LDH) remains as a topic of great interest in cancer study. Though a number of kinetic models have been applied to describe the dynamic behavior of LDH, few can reflect its actual mechanism, making it difficult to explain the observed substrate a...