Surface Plasmon Resonance kinetic analysis of the interaction between G-quadruplex nucleic acids and an anti-G-quadruplex monoclonal antibody

• Published in: Biochimica et biophysica acta Vol. 1862; no. 6; pp. 1276 - 1282
• Main Authors: Lago, Sara, Nadai, Matteo, Rossetto, Monica, Richter, Sara N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2018; Elsevier Pub. Co
• Subjects: Animals; Anti-G4 antibody; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - metabolism; Antibody Affinity; Antibody Specificity; antigens; enzyme-linked immunosorbent assay; G-quadruplex; G-Quadruplexes; Immobilization; in vitro studies; Kinetics; ligands; Mice; monitoring; monoclonal antibodies; nucleic acids; oligonucleotides; Oriented capturing; Specificity; Surface Plasmon Resonance; Surface Plasmon Resonance - methods; Surface Plasmon Resonance; Ab; RT; Specificity; RU; RII; SPR; G-quadruplex; Immobilization; Oriented capturing; Anti-G4 antibody; G4

G-quadruplexes (G4s) are nucleic acids secondary structures formed in guanine-rich sequences. Anti-G4 antibodies represent a tool for the direct investigation of G4s in cells. Surface Plasmon Resonance (SPR) is a highly sensitive technology, suitable for assessing the affinity between biomolecules....