In vivo imaging of embryonic stem cells reveals patterns of survival and immune rejection following transplantation

• Published in: Stem cells and development Vol. 17; no. 6; p. 1023
• Main Authors: Swijnenburg, Rutger-Jan, Schrepfer, Sonja, Cao, Feng, Pearl, Jeremy I, Xie, Xiaoyan, Connolly, Andrew J, Robbins, Robert C, Wu, Joseph C
• Format: Journal Article
• Language: English
• Published: United States 01.12.2008
• Subjects: Animals; Cell Survival - immunology; Diagnostic Imaging; Embryonic Stem Cells - immunology; Embryonic Stem Cells - metabolism; Embryonic Stem Cells - pathology; Genes, Reporter - immunology; Graft Rejection - genetics; Graft Rejection - immunology; Graft Rejection - metabolism; Graft Rejection - pathology; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - immunology; Luciferases, Firefly - biosynthesis; Luciferases, Firefly - genetics; Luciferases, Firefly - immunology; Luminescent Measurements; Mice; Mice, Inbred BALB C; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - immunology; Stem Cell Transplantation; Teratoma - genetics; Teratoma - immunology; Teratoma - metabolism; Teratoma - pathology; Transplantation, Homologous; Transplantation, Isogeneic

Embryonic stem cell (ESC)-based transplantation is considered a promising novel therapy for a variety of diseases. This is bolstered by the suggested immune-privileged properties of ESCs. In this study, we used in vivo bioluminescent imaging (BLI) to non-invasively track the fate of transplanted mur...