Occludin deficiency promotes ethanol-induced disruption of colonic epithelial junctions, gut barrier dysfunction and liver damage in mice

• Published in: Biochimica et biophysica acta Vol. 1860; no. 4; pp. 765 - 774
• Main Authors: Mir, Hina, Meena, Avtar S., Chaudhry, Kamaljit K., Shukla, Pradeep K., Gangwar, Ruchika, Manda, Bhargavi, Padala, Mythili K., Shen, Le, Turner, Jerrold R., Dietrich, Paula, Dragatsis, Ioannis, Rao, RadhaKrishna
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2016
• Subjects: Actin; Adherens junction; Alcohol; Animals; body weight changes; Caco-2 Cells; cadherins; Claudin; colon; Colon - metabolism; Colon - pathology; confocal microscopy; diet; digestive system diseases; endotoxemia; ethanol; Ethanol - adverse effects; Ethanol - pharmacology; Fatty liver; histopathology; human cell lines; Humans; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; inulin; Inulin - pharmacokinetics; Inulin - pharmacology; liver; Liver - metabolism; Liver - pathology; Liver Diseases - genetics; Liver Diseases - metabolism; Liver Diseases - pathology; Mice; Mice, Knockout; microfilaments; mucosa; Occludin - deficiency; Occludin - metabolism; occludins; pathogenesis; permeability; Permeability - drug effects; Tight junction; tight junctions; Tight Junctions - genetics; Tight Junctions - metabolism; Toll-like receptor 4; transmembrane proteins; triacylglycerols; Triglycerides - genetics; Triglycerides - metabolism; TER; EF; HRP; ALT; Alcohol; LPS; ECL; Adherens junction; FITC; Cldn; Ocln; ZO-1; Fatty liver; shRNA; Actin; PF; Claudin; Tight junction; WT

Disruption of epithelial tight junctions (TJ), gut barrier dysfunction and endotoxemia play crucial role in the pathogenesis of alcoholic tissue injury. Occludin, a transmembrane protein of TJ, is depleted in colon by alcohol. However, it is unknown whether occludin depletion influences alcoholic gu...