An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
The structural diversity of glycans on cells—the glycome—is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates...
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| Published in | Molecular cell Vol. 75; no. 2; pp. 394 - 407.e5 |
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| Main Authors | , , , , , , , , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
25.07.2019
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The structural diversity of glycans on cells—the glycome—is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.
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•Human glycosyltransferases (170 GTf genes) organized in glycosylation pathway maps•The human glycome displayed in a natural context on the cell surface•Sustainable cell-based array resource to dissect biological functions of glycans•Microbial adhesins may bind to clustered patches of O-glycans
Narimatsu et al. display the diversity of human sugars on the surface of a library of cells by genetically engineering the cellular glycosylation machinery. Sugars on the cell surface play important roles in interactions with the environment, and the cell library developed opens for studies of biological interactions with sugars. |
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| AbstractList | The structural diversity of glycans on cells—the glycome—is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.
[Display omitted]
•Human glycosyltransferases (170 GTf genes) organized in glycosylation pathway maps•The human glycome displayed in a natural context on the cell surface•Sustainable cell-based array resource to dissect biological functions of glycans•Microbial adhesins may bind to clustered patches of O-glycans
Narimatsu et al. display the diversity of human sugars on the surface of a library of cells by genetically engineering the cellular glycosylation machinery. Sugars on the cell surface play important roles in interactions with the environment, and the cell library developed opens for studies of biological interactions with sugars. The structural diversity of glycans on cells—the glycome—is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins. The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins. The structural diversity of glycans on cells – the glycome – is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logic sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins. Narimatsu and colleagues display the diversity of human sugars on the surface of a library of cells by genetically engineering the cellular glycosylation machinery. Sugars on the cell surface play important roles in interactions with the environment, and the cell library developed opens for studies of biological interactions with sugars. |
| Author | Furukawa, Sanae Thompson, Andrew J. Schjoldager, Katrine T. Bennett, Eric Paul Vakhrushev, Sergey Y. Van Coillie, Julie Sun, Lingbo Steentoft, Catharina Mandel, Ulla Varki, Ajit Joshi, Hiren J. Nason, Rebecca Büll, Christian Narimatsu, Yoshiki Ye, Zilu Bensing, Barbara A. Paulson, James C. Hansen, Lars Clausen, Henrik Adema, Gosse J. Karlsson, Richard Chen, Yen-Hsi Sullam, Paul M. Yang, Zhang |
| AuthorAffiliation | 5 Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA 4 Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA 6 Radiotherapy and OncoImmunology Laboratory, Department of Radiotherapy, Radboud University Medical Center, Nijmegen, The Netherlands 7 The Glycobiology Research and Training Center, and the Department of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USA 8 Lead Contact 3 Department of Medicine, The San Francisco Veterans Affairs Medical Center, and the University of California, San Francisco, CA 94121, USA 2 GlycoDisplay ApS, Copenhagen, Denmark 1 Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark |
| AuthorAffiliation_xml | – name: 1 Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – name: 5 Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA – name: 6 Radiotherapy and OncoImmunology Laboratory, Department of Radiotherapy, Radboud University Medical Center, Nijmegen, The Netherlands – name: 3 Department of Medicine, The San Francisco Veterans Affairs Medical Center, and the University of California, San Francisco, CA 94121, USA – name: 4 Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA – name: 2 GlycoDisplay ApS, Copenhagen, Denmark – name: 7 The Glycobiology Research and Training Center, and the Department of Cellular and Molecular Medicine, University of California, San Diego, CA 92093, USA – name: 8 Lead Contact |
| Author_xml | – sequence: 1 givenname: Yoshiki surname: Narimatsu fullname: Narimatsu, Yoshiki email: yoshiki@sund.ku.dk organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 2 givenname: Hiren J. surname: Joshi fullname: Joshi, Hiren J. organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 3 givenname: Rebecca surname: Nason fullname: Nason, Rebecca organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 4 givenname: Julie surname: Van Coillie fullname: Van Coillie, Julie organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 5 givenname: Richard surname: Karlsson fullname: Karlsson, Richard organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 6 givenname: Lingbo surname: Sun fullname: Sun, Lingbo organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 7 givenname: Zilu surname: Ye fullname: Ye, Zilu organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 8 givenname: Yen-Hsi surname: Chen fullname: Chen, Yen-Hsi organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 9 givenname: Katrine T. surname: Schjoldager fullname: Schjoldager, Katrine T. organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 10 givenname: Catharina surname: Steentoft fullname: Steentoft, Catharina organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 11 givenname: Sanae surname: Furukawa fullname: Furukawa, Sanae organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 12 givenname: Barbara A. surname: Bensing fullname: Bensing, Barbara A. organization: Department of Medicine, The San Francisco Veterans Affairs Medical Center, and the University of California, San Francisco, San Francisco, CA 94121, USA – sequence: 13 givenname: Paul M. surname: Sullam fullname: Sullam, Paul M. organization: Department of Medicine, The San Francisco Veterans Affairs Medical Center, and the University of California, San Francisco, San Francisco, CA 94121, USA – sequence: 14 givenname: Andrew J. surname: Thompson fullname: Thompson, Andrew J. organization: Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA – sequence: 15 givenname: James C. surname: Paulson fullname: Paulson, James C. organization: Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA – sequence: 16 givenname: Christian surname: Büll fullname: Büll, Christian organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 17 givenname: Gosse J. surname: Adema fullname: Adema, Gosse J. organization: Radiotherapy and OncoImmunology Laboratory, Department of Radiotherapy, Radboud University Medical Center, Nijmegen, the Netherlands – sequence: 18 givenname: Ulla surname: Mandel fullname: Mandel, Ulla organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 19 givenname: Lars surname: Hansen fullname: Hansen, Lars organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 20 givenname: Eric Paul surname: Bennett fullname: Bennett, Eric Paul organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 21 givenname: Ajit surname: Varki fullname: Varki, Ajit organization: The Glycobiology Research and Training Center and the Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, CA 92093, USA – sequence: 22 givenname: Sergey Y. surname: Vakhrushev fullname: Vakhrushev, Sergey Y. organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 23 givenname: Zhang surname: Yang fullname: Yang, Zhang organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark – sequence: 24 givenname: Henrik surname: Clausen fullname: Clausen, Henrik email: hclau@sund.ku.dk organization: Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, Copenhagen, Denmark |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31227230$$D View this record in MEDLINE/PubMed |
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| ContentType | Journal Article |
| Copyright | 2019 Elsevier Inc. Copyright © 2019 Elsevier Inc. All rights reserved. |
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| Keywords | microarray adhesin glycoengineering glycosyltransferase lectin galectin glycosylation glycan array siglec carbohydrate |
| Language | English |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 AUTHOR CONTRIBUTIONS Y.N. and H.C.; conceived and designed the study; Y-H.C., H.J.J., R.N., R.K., J.V.C., L.S., Z.Y., Y-H.C., K.T.S., S.F., U.M., L.H., E.P.B., S.Y.V. and Z.Y. contributed with experimental data and interpretation; B.A.B., P.M.S., and A.V. contributed to the streptococcal adhesin studies; A.J.T. and J.C.P. contributed to the influenza HA studies; C.B. and G.J.A. contributed to the Siglec studies; Y.N. and H.C wrote the manuscript, and all authors edited and approved the final version. |
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| SubjectTerms | adhesin adhesins biosynthesis carbohydrate Epitopes - genetics Epitopes - immunology galectin genes Genetic Engineering glycan array glycoconjugates glycoengineering Glycosylation glycosyltransferase Glycosyltransferases - genetics haptens HEK293 Cells human cell lines Humans lectin Metabolic Networks and Pathways - genetics microarray oligosaccharides Oligosaccharides - genetics polysaccharides Polysaccharides - chemistry Polysaccharides - classification Polysaccharides - genetics Polysaccharides - immunology Proteins - genetics Proteins - immunology siglec transferases |
| Title | An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells |
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