Characterization of constitutive and acid-induced outwardly rectifying chloride currents in immortalized mouse distal tubular cells

• Published in: Biochimica et biophysica acta Vol. 1861; no. 8; pp. 2007 - 2019
• Main Authors: Valinsky, William C., Touyz, Rhian M., Shrier, Alvin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2017; Elsevier Pub. Co
• Subjects: absorption; Acid-induced chloride current; Acid-sensitive outwardly rectifying (ASOR) anion channel; Animals; anions; benzoic acid; bromides; calcium; Cells, Cultured; Chloride Channels - physiology; Chloride current; chlorides; Chlorides - metabolism; Distal convoluted tubule; Hydrogen-Ion Concentration; Kidney Tubules, Distal - metabolism; kidneys; MDCT cells; Mice; mIMCD-3 cells; plasma membrane; sodium; FFA; Chloride current; MDCT cells; Acid-sensitive outwardly rectifying (ASOR) anion channel; HBE; Acid-induced chloride current; MDCT; mIMCD-3; Distal convoluted tubule; 2-APB; HEK; NCC; NPPB; I-V; DCT; DIDS; VLJP; VRAC; mIMCD-3 cells; TRPM

Thiazides block Na+ reabsorption while enhancing Ca2+ reabsorption in the kidney. As previously demonstrated in immortalized mouse distal convoluted tubule (MDCT) cells, chlorothiazide application induced a robust plasma membrane hyperpolarization, which increased Ca2+ uptake. This essential thiazid...