Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysates of Tilapia (Oreochromis niloticus) Frame Protein

• Published in: Molecules (Basel, Switzerland) Vol. 17; no. 11; pp. 12836 - 12850
• Main Authors: Fan, Jian, He, Jintang, Zhuang, Yongliang, Sun, Liping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.11.2012; MDPI
• Subjects: Amino Acid Sequence; Animals; antioxidant peptide; Antioxidants; Biphenyl Compounds - chemistry; Chromatography; Chromatography, Gel; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Chromatography, Reverse-Phase; Cichlids; enzymatic hydrolysis; Fish Proteins - chemistry; Fish Proteins - isolation & purification; Free Radical Scavengers - chemistry; Free Radical Scavengers - isolation & purification; Free radicals; Free Radicals - chemistry; Hydrogen Peroxide - chemistry; Hydrolysis; Peptide Fragments - chemistry; Peptide Fragments - isolation & purification; Peptide Hydrolases - chemistry; Peptides; Picrates - chemistry; Proteins; Proteolysis; purification; Skin; Superoxides - chemistry; Tilapia; tilapia frame protein; Ultrafiltration

Tilapia frame protein was hydrolyzed by different proteases, including properase E, pepsin, trypsin, flavourzyme, neutrase, gc106 and papain, to obtain antioxidant peptides. The tilapia frame protein hydrolysate (TFPH) obtained by trypsin exhibited the highest degree of hydrolysis and antioxidant ac...