The use of PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) to detect bacterial and fungal colonization in healthy military service members

• Published in: BMC infectious diseases Vol. 16; no. 1; p. 338
• Main Authors: Vetor, Ryan, Murray, Clinton K., Mende, Katrin, Melton-Kreft, Rachel, Akers, Kevin S., Wenke, Joseph, Spirk, Tracy, Guymon, Charles, Zera, Wendy, Beckius, Miriam L., Schnaubelt, Elizabeth R., Ehrlich, Garth, Vento, Todd J.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 22.07.2016; BioMed Central
• Subjects: Adult; Analysis; Bacteria - genetics; Bacteria - growth & development; Bacteria - isolation & purification; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - isolation & purification; Female; Fungi - genetics; Fungi - growth & development; Fungi - isolation & purification; Gram-Negative Bacteria - genetics; Gram-Negative Bacteria - growth & development; Gram-Negative Bacteria - isolation & purification; Health; Humans; Infectious diseases; Male; Microbial colonies; Microbiological Techniques - methods; Microbiota; Military Personnel; Molecular Diagnostic Techniques - methods; Pilot Projects; Polymerase Chain Reaction - methods; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staphylococcus aureus - genetics; Staphylococcus aureus - growth & development; Staphylococcus aureus - isolation & purification; Time-of-flight mass spectrometry; Virulence (Microbiology); Young Adult; Bacterial; Molecular diagnostics; Electrospray ionization time-of-flight mass spectrometry; Fungal; Colonization; PCR; Military

The role of microbial colonization in disease is complex. Novel molecular tools to detect colonization offer theoretical improvements over traditional methods. We evaluated PCR/Electrospray Ionization-Time-of-Flight-Mass Spectrometry (PCR/ESI-TOF-MS) as a screening tool to study colonization of heal...